##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548054_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1564158
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55029287322636	31.0	31.0	33.0	30.0	34.0
2	31.941783374825306	31.0	31.0	34.0	30.0	34.0
3	31.989420506112555	33.0	31.0	34.0	30.0	34.0
4	35.702455250684395	37.0	35.0	37.0	33.0	37.0
5	35.5953803899606	37.0	35.0	37.0	33.0	37.0
6	35.668017553213936	37.0	35.0	37.0	33.0	37.0
7	36.07101840095438	37.0	35.0	37.0	35.0	37.0
8	36.11291570288935	37.0	35.0	37.0	35.0	37.0
9	37.98500343315701	39.0	39.0	39.0	35.0	39.0
10	37.37703352218894	39.0	37.0	39.0	35.0	39.0
11	36.963309972521955	39.0	37.0	39.0	33.0	39.0
12	35.575808837726115	35.0	35.0	39.0	32.0	39.0
13	34.92029513642483	35.0	35.0	39.0	30.0	39.0
14	35.75113511550624	36.0	35.0	40.0	30.0	41.0
15	36.19765969933984	36.0	35.0	40.0	31.0	41.0
16	36.490027222313856	36.0	35.0	40.0	32.0	41.0
17	36.46030388234437	36.0	35.0	40.0	32.0	41.0
18	36.418067739959774	36.0	35.0	40.0	32.0	41.0
19	36.34503483663415	36.0	35.0	40.0	32.0	41.0
20	36.1986755813671	36.0	35.0	40.0	32.0	41.0
21	35.999441232918926	35.0	35.0	40.0	31.0	41.0
22	35.88251059036236	35.0	35.0	40.0	31.0	41.0
23	35.891989811770934	35.0	35.0	40.0	31.0	41.0
24	35.86768024713616	35.0	35.0	40.0	31.0	41.0
25	35.852039883438884	35.0	35.0	40.0	31.0	41.0
26	35.69378413178208	35.0	35.0	40.0	31.0	41.0
27	35.62976502373801	35.0	34.0	40.0	31.0	41.0
28	35.649501520946096	36.0	35.0	40.0	31.0	41.0
29	35.693231118595435	36.0	35.0	40.0	31.0	41.0
30	35.658060119246265	36.0	35.0	40.0	31.0	41.0
31	35.46508984386488	35.0	34.0	40.0	30.0	41.0
32	35.2616570704494	35.0	34.0	40.0	30.0	41.0
33	35.128137310936616	35.0	34.0	40.0	30.0	41.0
34	35.042904233459794	35.0	34.0	40.0	29.0	41.0
35	34.901519539586154	35.0	34.0	40.0	29.0	41.0
36	34.64689948202164	35.0	34.0	40.0	27.0	41.0
37	34.51697846381248	35.0	34.0	40.0	27.0	41.0
38	34.46980292272264	35.0	33.0	40.0	27.0	41.0
39	34.4844165359254	35.0	34.0	40.0	27.0	41.0
40	34.29989169892044	35.0	34.0	40.0	25.0	41.0
41	34.31551607957764	35.0	34.0	40.0	25.0	41.0
42	34.2797242989519	35.0	34.0	40.0	25.0	41.0
43	34.11408885803097	35.0	33.0	40.0	25.0	41.0
44	33.96767909635727	35.0	33.0	39.0	24.0	41.0
45	33.90546095726902	35.0	33.0	39.0	24.0	41.0
46	33.8475934016896	35.0	33.0	39.0	24.0	41.0
47	33.773498585181294	35.0	33.0	39.0	23.0	41.0
48	33.71761420521456	35.0	33.0	39.0	23.0	41.0
49	33.68578366124138	35.0	33.0	39.0	24.0	41.0
50	33.45532228841332	35.0	33.0	39.0	24.0	41.0
51	33.01753850953676	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	3.0
14	8.0
15	27.0
16	77.0
17	269.0
18	596.0
19	1248.0
20	2303.0
21	3822.0
22	6097.0
23	9023.0
24	13035.0
25	18471.0
26	24160.0
27	28504.0
28	31867.0
29	36200.0
30	42684.0
31	52954.0
32	66828.0
33	89159.0
34	180287.0
35	328272.0
36	83628.0
37	101468.0
38	155963.0
39	287156.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.81270690045379	24.156958568124193	31.156571139232735	12.87376339218928
2	28.409342278721205	26.938582930880383	32.316939848787655	12.335134941610757
3	27.208056986570412	25.951662172235796	32.559434532828526	14.280846308365266
4	25.955881694815996	28.092110899282552	31.54502294525233	14.406984460649117
5	23.260757544953897	33.207003384568566	29.78970155188926	13.742537518588277
6	20.78351419741484	41.33118265546064	26.739562115847633	11.145741031276891
7	89.48085807188276	3.8962176455319733	4.955317813162098	1.6676064694231656
8	91.17589143807723	2.495975470508734	4.374110543819742	1.9540225475942967
9	88.3949063969241	3.938860396456113	5.773777329400227	1.892455877219565
10	58.93458333493163	21.669933600058304	10.380409140253095	9.015073924756962
11	54.950586833299454	17.84851658208442	16.28678177012808	10.91411481448805
12	49.03289821105029	19.831372533976747	18.59428523205456	12.541444022918402
13	21.224646103526627	47.21703306187738	19.477763755323952	12.080557079272044
14	13.233893251193294	49.80174637089092	24.703386742260054	12.260973635655734
15	10.654614175805769	23.25135951738891	54.26414722809333	11.829879078711997
16	12.631524436789634	16.868628361073498	52.634516461891955	17.86533074024491
17	13.680075797969259	17.1726897154891	27.089398897042372	42.05783558949927
18	21.526150171529988	21.80003554628113	35.19363133391895	21.48018294826993
19	27.746301844187094	24.66943876513754	26.059196065870584	21.525063324804783
20	32.30939585387154	22.022199803344673	23.301162670267324	22.367241672516457
21	20.71363634620032	27.860804343295243	28.145238524496886	23.280320786007554
22	22.300752225798163	22.680189597214603	26.03899350321387	28.980064673773363
23	17.877222122061838	30.57076075434835	23.540908271415038	28.011108852174782
24	20.20090042054575	22.412889234974983	39.34839063572861	18.03781970875065
25	14.517714962299205	23.857756057891848	37.63021382750336	23.994315152305585
26	14.67927153139261	37.191191682681676	27.206139021761228	20.923397764164488
27	16.50210528603888	35.20948650967485	30.189661146763946	18.09874705752232
28	12.003966351225388	29.02398606790363	42.799512581209825	16.17253499966116
29	13.537443148326448	23.412788222161698	41.59439135944067	21.455377270071182
30	17.24806573248994	29.809904114545972	33.62550330593201	19.31652684703208
31	30.816643842885437	25.35990609644294	23.592053999659882	20.23139606101174
32	30.929675902306546	27.966803865082685	25.383816724397406	15.719703508213364
33	27.491404321046854	28.61782505347925	25.22590428844145	18.66486633703245
34	18.8438124537291	29.358734859266132	28.083160396839705	23.71429229016506
35	19.211102714687392	26.72063819639704	31.358596765799874	22.709662323115694
36	32.19572447284737	20.451706285426408	29.822498750126265	17.530070491599954
37	20.250383912622638	29.83138532040881	31.398106840869016	18.520123926099537
38	20.63921931160407	31.23514376424888	23.229878311526072	24.895758612620973
39	19.25035706111531	30.443280026698073	29.880485219523862	20.425877692662763
40	23.544360608071564	26.186996454322394	25.67867184772894	24.58997108987711
41	16.73424296011017	23.089802948295503	28.857187061665122	31.3187670299292
42	25.042802581324903	24.06278649599337	24.069435440665202	26.824975482016523
43	24.524696354204625	25.16926039441028	25.575805001796493	24.730238249588595
44	19.94248662858867	32.1495654531064	27.01517365892704	20.892774259377887
45	16.5899480742994	39.29417616378908	21.68949684111196	22.42637892079956
46	24.862130296299988	30.861907812382128	26.304248036323695	17.97171385499419
47	22.874223703743485	27.584553478612772	26.738539201282734	22.80268361636101
48	25.483039437192406	21.82548054608294	29.9696066509905	22.72187336573415
49	21.51195723194204	20.178460232278326	33.06283636307841	25.246746172701222
50	19.474822875949872	31.400216602159116	27.54510733570394	21.579853186187073
51	17.89384448374141	34.97172280549663	22.8416822341477	24.292750476614255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	721.0
1	1022.5
2	1324.0
3	4172.5
4	7021.0
5	5659.0
6	4297.0
7	4576.5
8	4856.0
9	5293.0
10	5730.0
11	5718.0
12	5706.0
13	5451.5
14	5197.0
15	4873.0
16	4549.0
17	4430.5
18	4312.0
19	3844.0
20	3376.0
21	3289.5
22	3203.0
23	3003.5
24	2804.0
25	4016.5
26	6588.5
27	7948.0
28	8171.5
29	8395.0
30	11141.5
31	13888.0
32	18151.5
33	22415.0
34	24252.5
35	26090.0
36	30049.0
37	34008.0
38	45160.5
39	56313.0
40	89330.0
41	122347.0
42	152874.0
43	183401.0
44	192167.5
45	200934.0
46	191283.0
47	181632.0
48	171531.0
49	161430.0
50	150000.0
51	138570.0
52	122437.0
53	106304.0
54	91829.0
55	77354.0
56	63856.5
57	50359.0
58	43133.0
59	35907.0
60	32297.0
61	28687.0
62	24343.0
63	19999.0
64	16022.5
65	12046.0
66	9748.0
67	7450.0
68	5914.0
69	4378.0
70	3642.0
71	2906.0
72	2219.5
73	1533.0
74	1164.0
75	650.0
76	505.0
77	311.0
78	117.0
79	86.0
80	55.0
81	38.5
82	22.0
83	22.5
84	23.0
85	16.5
86	10.0
87	8.0
88	6.0
89	3.5
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1564158.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.23060154975819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92262774630086	15.561908105485186
2	8.654613728710162	3.3286685636778426
3	2.861278958659715	1.6507234673007607
4	1.3332329805313905	1.0255548888638275
5	0.6940696077579132	0.6673688037294693
6	0.45952672252489296	0.5302185181405505
7	0.3412007171874402	0.4593046528506376
8	0.24011826933960273	0.3694095009989935
9	0.18149298372683323	0.31411973287147293
>10	1.7669629398556346	8.032269011750506
>50	0.8832488729845425	12.655888892460322
>100	1.654635056688573	53.64059327338185
>500	0.005659717497727497	0.7218637824916084
>1k	9.987736760695583E-4	0.47671820172350476
>5k	3.3292455868985275E-4	0.5653906042734717
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8831	0.5645849076627809	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4316	0.2759312038809379	No Hit
AGCTGAGGGAAATGTATTTTGAAAACATTTCACACTTCAACTTTGTATCCA	1627	0.10401762481795317	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08860997418419367	0.0
2	0.0	0.0	0.0	0.3112217563698808	0.0
3	0.0	0.0	0.0	0.46159019740972457	0.0
4	0.0	0.0	0.0	0.7363706224051535	0.0
5	0.0	0.0	0.0	1.194572415318657	0.0
6	0.0	0.0	0.0	1.6550118338428728	0.0
7	0.0	0.0	0.0	1.92365477144892	0.0
8	0.0	0.0	0.0	2.7498500790840823	0.0
9	0.0	0.0	0.0	3.0231600643924716	0.0
10	0.0	0.0	0.0	3.56639163051303	0.0
11	0.0	0.0	0.0	4.085904365160041	0.0
12	0.0	0.0	0.0	4.5353474521116155	0.0
13	0.0	0.0	0.0	4.719344209472444	0.0
14	0.0	0.0	0.0	4.776947085908201	0.0
15	0.0	0.0	0.0	4.890618466932369	0.0
16	0.0	0.0	0.0	5.14040141724813	0.0
17	0.0	0.0	0.0	5.419848889945901	0.0
18	0.0	0.0	0.0	5.885850406416743	0.0
19	0.0	0.0	0.0	6.055078834746873	0.0
20	0.0	0.0	0.0	6.250966973924629	0.0
21	0.0	0.0	0.0	6.498256569988454	0.0
22	0.0	0.0	0.0	6.727517296845971	0.0
23	0.0	0.0	0.0	7.000763349994054	0.0
24	0.0	0.0	0.0	7.186486275683147	0.0
25	0.0	0.0	0.0	7.336598988081767	0.0
26	0.0	0.0	0.0	7.500840707908025	0.0
27	0.0	0.0	0.0	7.6368244128790055	0.0
28	6.393216030605604E-5	0.0	0.0	7.798764574934245	0.0
29	6.393216030605604E-5	0.0	0.0	7.969015917829273	0.0
30	6.393216030605604E-5	0.0	0.0	8.197445526602811	0.0
31	6.393216030605604E-5	0.0	0.0	8.414814871643403	0.0
32	6.393216030605604E-5	0.0	0.0	8.584682621576592	0.0
33	6.393216030605604E-5	0.0	0.0	8.767528600051913	0.0
34	6.393216030605604E-5	0.0	0.0	8.95868575936702	0.0
35	6.393216030605604E-5	0.0	0.0	9.207701523759109	0.0
36	6.393216030605604E-5	0.0	0.0	9.404037188059007	0.0
37	6.393216030605604E-5	0.0	0.0	9.60043678451921	0.0
38	6.393216030605604E-5	0.0	0.0	9.789739911185443	0.0
39	6.393216030605604E-5	0.0	0.0	9.978915173531062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.0340675E-4	45.000004	36
ACTATCG	40	6.8175723E-9	45.000004	16
ACACGGC	20	7.0340675E-4	45.000004	36
CATATTA	20	7.0340675E-4	45.000004	32
CGTATCA	20	7.0340675E-4	45.000004	40
TGGCGTA	20	7.0340675E-4	45.000004	1
CGTTCCC	20	7.0340675E-4	45.000004	25
CTAAACG	40	6.8175723E-9	45.000004	45
ACGGACG	20	7.0340675E-4	45.000004	39
ACAGCGT	20	7.0340675E-4	45.000004	30
CTCGAAG	20	7.0340675E-4	45.000004	42
CCGGTTG	20	7.0340675E-4	45.000004	1
AACTCGT	20	7.0340675E-4	45.000004	29
CACAATA	20	7.0340675E-4	45.000004	33
CGCGTGA	20	7.0340675E-4	45.000004	4
ATACGCG	20	7.0340675E-4	45.000004	1
TTCGCTA	20	7.0340675E-4	45.000004	14
TACGTCA	20	7.0340675E-4	45.000004	17
CGAAGTC	20	7.0340675E-4	45.000004	31
TATCCGA	20	7.0340675E-4	45.000004	24
>>END_MODULE