FastQCFastQC Report
Thu 26 May 2016
SRR1548053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548053_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3166843
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100660.31785598465095993No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC65360.20638850741890266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT302.1667129E-645.00000421
CGACGGT4500.042.50000428
GTTGACG1250.041.3999981
TATAGCG603.6379788E-1241.2500041
CTCACGA4900.040.86734824
GCGTAAG1900.040.2631571
CGGTCTA4700.040.21276531
ACGGTCT4700.040.21276530
TAGGACG1600.039.3751
ATCGATC403.460791E-739.37516
CGTTTTT60850.039.3426481
TAGTGCG1550.039.1935461
ACGGGAT13500.039.05
ACATACG5450.038.8073417
AACGGGA11950.038.5983284
CGCGTAG356.2517647E-638.57142642
CGTAAGG6600.038.181822
TAGGGAC26700.037.837085
GGGCGAT37750.037.6688777
CACGACC2450.037.6530627
Produced by FastQC (version 0.11.5)