FastQCFastQC Report
Thu 26 May 2016
SRR1548052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548052_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2427688
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.3373168216014578No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39340.16204718233974053No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26890.11076382138067165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG302.1665037E-645.0000041
TCATACG453.8562575E-1045.0000041
TAGTCCG253.8920916E-545.0000041
CTATCGT207.034839E-445.027
GCGATCG207.034839E-445.09
CGTAAGG3850.041.4935042
CGTTAGG4400.040.3977282
TGCGAAG3900.040.3846171
TACGGGA9800.040.178574
CACGACG3200.040.07812526
TCGGACG451.9297659E-840.0000041
AACGGGA9550.039.5811544
CGGTCTA3300.039.54545631
AATACGG1900.039.0789452
CGTTTTT49350.039.027361
CGACGGT3250.038.7692328
ACGGGAT13050.038.6206935
TTGCGAG2100.038.571431
CGAACGG1400.038.5714262
TAAGTCG700.038.5714261
Produced by FastQC (version 0.11.5)