FastQCFastQC Report
Thu 26 May 2016
SRR1548051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548051_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1336622
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35890.26851271339241756No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30060.22489529575302514No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22230.16631478458382398No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16210.12127587305909975No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13680.10234755974389169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG502.1827873E-1145.0000041
CTCGTTA207.0336985E-445.00000437
ATTACGA650.045.00000412
AAACGCG253.8911472E-545.0000041
CGCTAAA302.1657688E-645.00000428
CCGTTAT253.8911472E-545.00000430
GCGTAAC406.8157533E-945.00000432
TACGTAG406.8157533E-945.0000041
ACGGTAC207.0336985E-445.00000437
GCGATAA1100.042.9545449
ATTACGG900.042.52
CGACGGT1700.041.0294128
TGTTCGG556.184564E-1140.9090922
CGAGGGA7200.040.6254
ACGTAAG501.0822987E-940.5000041
ACGCTAG451.9286745E-840.01
CGCGTAA451.9286745E-840.031
CGGTCTA1700.039.70588331
CATACGA5100.039.26470618
CGCATGG1800.038.752