FastQCFastQC Report
Thu 26 May 2016
SRR1548050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548050_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2478113
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372891.5047336420897677No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35310.14248744911955186No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33010.13320619358358557No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT28440.11476474236646998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1000.045.06
TAATTCG207.034868E-445.018
AGTCGTA207.034868E-445.036
CGTTTTT171850.043.4812361
CGACGGT2250.043.028
TTCGTAG1850.042.5675661
AGGGCGA15500.041.806456
TACGGGA11700.040.5769234
CGGTCCG501.0822987E-940.529
CGGTCTA2450.040.40816531
CGGTTTT15750.040.1428571
ACGGGAT12450.040.1204835
TCTTGCG451.9297659E-840.01
ACGCCGA1250.039.612
GGGCGAT29500.039.5847477
CGTTCGA403.4603727E-739.37514
TAGGGCG6500.038.769235
AATGCGG3600.038.752
TAGCGCG1050.038.571431
CGAAAGG2400.038.4375042