##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548050_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2478113
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477431820098598	31.0	31.0	33.0	30.0	34.0
2	31.849307517453806	31.0	31.0	34.0	30.0	34.0
3	31.701951444506363	31.0	31.0	34.0	30.0	34.0
4	35.54149064227499	37.0	35.0	37.0	33.0	37.0
5	35.532024165161154	37.0	35.0	37.0	33.0	37.0
6	35.633427934884324	37.0	35.0	37.0	33.0	37.0
7	36.03751887020487	37.0	35.0	37.0	35.0	37.0
8	36.07439007018647	37.0	35.0	37.0	35.0	37.0
9	37.894172299648964	39.0	38.0	39.0	35.0	39.0
10	37.34731507400994	39.0	37.0	39.0	34.0	39.0
11	36.9693775061912	39.0	37.0	39.0	33.0	39.0
12	36.46954033169593	39.0	35.0	39.0	32.0	39.0
13	36.25937961666801	39.0	35.0	39.0	32.0	39.0
14	37.35296130563861	40.0	35.0	41.0	32.0	41.0
15	37.55377418221042	40.0	35.0	41.0	33.0	41.0
16	37.64960718094776	40.0	35.0	41.0	33.0	41.0
17	37.57860557609762	39.0	35.0	41.0	33.0	41.0
18	37.413778951968695	39.0	36.0	41.0	32.0	41.0
19	37.29807317099745	39.0	35.0	41.0	32.0	41.0
20	37.08687537654659	39.0	35.0	41.0	32.0	41.0
21	36.97362065410254	38.0	35.0	41.0	32.0	41.0
22	36.895294928035966	38.0	35.0	41.0	32.0	41.0
23	36.82829515845322	38.0	35.0	41.0	32.0	41.0
24	36.802689385028046	38.0	35.0	41.0	32.0	41.0
25	36.766751960059935	38.0	35.0	41.0	32.0	41.0
26	36.636161466406094	38.0	35.0	41.0	31.0	41.0
27	36.5687960960618	38.0	35.0	40.0	31.0	41.0
28	36.5009388191741	38.0	35.0	40.0	31.0	41.0
29	36.42173863742291	38.0	35.0	40.0	31.0	41.0
30	36.29826605969946	38.0	35.0	40.0	31.0	41.0
31	36.09908264877348	38.0	35.0	40.0	30.0	41.0
32	35.85377624022795	38.0	35.0	40.0	30.0	41.0
33	35.590815269521606	38.0	35.0	40.0	28.0	41.0
34	35.33009834499072	38.0	34.0	40.0	26.0	41.0
35	35.07034667103558	38.0	34.0	40.0	25.0	41.0
36	34.86077471043492	38.0	34.0	40.0	24.0	41.0
37	34.742478248570585	38.0	34.0	40.0	23.0	41.0
38	34.62345784877445	38.0	34.0	40.0	23.0	41.0
39	34.57002687125244	38.0	34.0	40.0	23.0	41.0
40	34.44283372065761	37.0	34.0	40.0	22.0	41.0
41	34.36230672289762	37.0	34.0	40.0	22.0	41.0
42	34.28860225502227	37.0	33.0	40.0	22.0	41.0
43	34.154689878952254	37.0	33.0	40.0	21.0	41.0
44	33.962583223606025	37.0	33.0	40.0	20.0	41.0
45	33.94894825215799	37.0	33.0	40.0	20.0	41.0
46	33.85709126258568	37.0	33.0	40.0	20.0	41.0
47	33.76641460659784	36.0	33.0	40.0	20.0	41.0
48	33.64183029587432	36.0	33.0	40.0	20.0	41.0
49	33.53000246558571	36.0	33.0	40.0	19.0	41.0
50	33.35851835650755	36.0	33.0	40.0	18.0	41.0
51	32.997050174870964	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	6.0
13	7.0
14	22.0
15	70.0
16	171.0
17	428.0
18	1049.0
19	2091.0
20	3742.0
21	6491.0
22	10449.0
23	15751.0
24	24214.0
25	37594.0
26	50545.0
27	55717.0
28	55703.0
29	57328.0
30	63742.0
31	73231.0
32	88743.0
33	112614.0
34	185222.0
35	288587.0
36	170623.0
37	211823.0
38	328648.0
39	633398.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.749768069494817	23.67478803428254	28.05816361077965	17.517280285442997
2	33.7722291114247	25.053821193787368	28.937058156750723	12.236891538037208
3	27.910389881333096	23.975097180798453	35.11732515829585	12.997187779572602
4	23.915495378943575	26.780417196471667	35.17531282875317	14.128774595831587
5	22.06909047327543	31.38521124742899	32.945228889885165	13.60046938941041
6	20.292133570987282	39.94866255090063	29.746181873062287	10.013022005049809
7	85.05399067758411	3.0559139151443055	10.383465160789681	1.5066302464818997
8	85.68475287446536	2.4326170759767614	10.10635915311368	1.7762708964441896
9	81.18318252638197	4.856073956272374	11.454764169349824	2.505979347995834
10	42.09243081328414	32.4331860572944	16.5210787401543	8.953304389267155
11	31.44566046826759	22.481904578201238	31.489040249577	14.583394703954177
12	31.01162053546388	21.420532477736085	31.273755474427517	16.294091512372518
13	21.72217328265499	30.649570862991315	31.454376777814407	16.173879076539286
14	15.851577389731622	34.51101705208762	32.60069254307612	17.036713015104638
15	14.794926623604333	27.104696194241345	42.829644975834434	15.27073220631989
16	18.554521121514636	24.4689003285968	41.108052780482566	15.868525769405995
17	18.626107848996394	23.817396543256905	32.215439731763645	25.341055875983052
18	19.943360129259645	26.25550166598537	35.30274850259048	18.498389702164513
19	23.187199292364795	27.820886295338425	30.4128181402543	18.579096272042477
20	24.800160444660918	27.35331278275042	29.908361725232062	17.9381650473566
21	21.360890322596266	27.646398691262263	32.7804664274793	18.212244558662174
22	20.883591668337964	24.55779861531738	32.78724577934904	21.77136393699561
23	17.941191543727022	29.13604020478485	31.914767405683275	21.00800084580485
24	18.197233136664874	26.925971495246586	37.25483866151382	17.62195670657472
25	18.14848636845858	28.042587242793203	34.60625080454362	19.202675584204595
26	17.529789803774083	32.182753570963065	30.849359976724227	19.438096648538625
27	17.677240706941127	31.074127773834366	33.079121089312714	18.169510429911792
28	16.0970867753004	28.54353292202575	37.624353691700094	17.735026610973755
29	16.964198162069284	26.550403472319463	36.67697962118757	19.80841874442368
30	18.858784889954574	28.051343905624964	34.39887527324218	18.690995931178282
31	23.516724217176538	27.22728947388598	30.021149156636522	19.234837152300965
32	23.561637423313623	28.262230172716095	30.277997815273157	17.898134588697125
33	21.64856889092628	29.36585216251236	30.34720369894351	18.638375247617844
34	18.429748764483296	29.112473886380485	31.82490064012416	20.632876709012056
35	18.064148002936108	28.920997549345007	32.51227849577481	20.50257595194408
36	22.635610240533826	27.985769817599117	30.1419265384589	19.236693403408157
37	18.827793567121436	30.295551494221613	31.83329412339147	19.043360815265487
38	18.625260430012673	31.21096576306246	28.785814044799412	21.377959762125457
39	18.23161413543289	30.080266719072135	30.489085848788978	21.199033296706002
40	20.786340251635014	28.6448196672226	29.088261915417096	21.48057816572529
41	17.450172772589468	27.63013631743185	30.735765479620987	24.183925430357693
42	20.432885828854456	28.306578432864036	28.613949404244277	22.64658633403723
43	20.439745887294084	28.935201905643527	28.911958413518672	21.713093793543717
44	19.458717177142447	31.299299103793892	29.138985994585397	20.10299772447826
45	17.99566847839465	33.23411805676335	27.25735267116552	21.512860793676477
46	21.03975888105183	31.23235300408012	28.221957594346993	19.50593052052106
47	19.97435145209278	28.97636225628129	30.246239780026173	20.803046511599753
48	20.986452191647434	27.083591426218256	30.485050520295083	21.44490586183923
49	19.41525668926316	26.73300208666836	31.281584011705682	22.570157212362794
50	18.429143465209215	29.97191814901096	30.65191135351778	20.947027032262046
51	17.781876774787914	31.912507621726693	28.500032080861526	21.805583522623866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1403.0
1	2184.5
2	2966.0
3	29057.5
4	55149.0
5	39144.5
6	23140.0
7	21445.0
8	19750.0
9	20782.5
10	21815.0
11	22643.5
12	23472.0
13	23440.0
14	23408.0
15	22298.0
16	21188.0
17	19527.5
18	17867.0
19	16749.5
20	15632.0
21	15717.0
22	15802.0
23	17302.5
24	18803.0
25	20320.5
26	25282.5
27	28727.0
28	35693.5
29	42660.0
30	47768.0
31	52876.0
32	58874.0
33	64872.0
34	72679.5
35	80487.0
36	87502.0
37	94517.0
38	105182.0
39	115847.0
40	138662.5
41	161478.0
42	180537.5
43	199597.0
44	206662.5
45	213728.0
46	219168.5
47	224609.0
48	211885.0
49	199161.0
50	191985.0
51	184809.0
52	163044.0
53	141279.0
54	125304.5
55	109330.0
56	96338.0
57	83346.0
58	74581.0
59	65816.0
60	57502.5
61	49189.0
62	41677.5
63	34166.0
64	27927.0
65	21688.0
66	16773.0
67	11858.0
68	9425.0
69	6992.0
70	5516.5
71	4041.0
72	2951.5
73	1862.0
74	1586.0
75	1012.5
76	715.0
77	543.5
78	372.0
79	314.5
80	257.0
81	172.5
82	88.0
83	95.5
84	103.0
85	69.5
86	36.0
87	29.0
88	22.0
89	20.5
90	19.0
91	18.5
92	18.0
93	11.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2478113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.40425335075074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91908755849322	20.302847478967927
2	7.190182914445549	3.6532245679362814
3	2.6456830585878013	2.0163480811846086
4	1.553237629616171	1.5783536902675497
5	0.957249945999932	1.2159110074087367
6	0.6928491591171486	1.0560789343239994
7	0.4933492496078227	0.8773218529207925
8	0.4035215872834469	0.8200931708676609
9	0.354672883927845	0.8109179819949948
>10	3.864914441646354	23.254129062502372
>50	1.4577780817645194	26.408461628574216
>100	0.46361678837410586	15.365720358464156
>500	0.0025711340906838776	0.44787559106876423
>1k	0.0011248711646741963	0.6704457963834032
>5k	0.0	0.0
>10k+	1.6069588066774235E-4	1.5222707971345502
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37289	1.5047336420897677	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3531	0.14248744911955186	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3301	0.13320619358358557	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	2844	0.11476474236646998	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.070656987796763E-5	0.0	0.0	0.04701157695391615	0.0
2	8.070656987796763E-5	0.0	0.0	0.16399574999203023	0.0
3	8.070656987796763E-5	0.0	0.0	0.3161679874969382	0.0
4	8.070656987796763E-5	0.0	0.0	0.461359106707402	0.0
5	8.070656987796763E-5	0.0	0.0	0.8838983533035015	0.0
6	8.070656987796763E-5	0.0	0.0	1.5049354085144624	0.0
7	8.070656987796763E-5	0.0	0.0	1.9665769882164372	0.0
8	8.070656987796763E-5	0.0	0.0	2.7734409205714186	0.0
9	8.070656987796763E-5	0.0	0.0	3.2406109003100343	0.0
10	8.070656987796763E-5	0.0	0.0	3.809995750799096	0.0
11	8.070656987796763E-5	0.0	0.0	4.202512153400591	0.0
12	8.070656987796763E-5	0.0	0.0	4.557822827288344	0.0
13	8.070656987796763E-5	0.0	0.0	4.728759342289879	0.0
14	8.070656987796763E-5	0.0	0.0	4.822742142912773	0.0
15	8.070656987796763E-5	0.0	0.0	4.915998584406764	0.0
16	8.070656987796763E-5	0.0	0.0	5.082415531495133	0.0
17	8.070656987796763E-5	0.0	0.0	5.278048256879327	0.0
18	8.070656987796763E-5	0.0	0.0	5.552531301034295	0.0
19	8.070656987796763E-5	0.0	0.0	5.691951900498484	0.0
20	8.070656987796763E-5	0.0	0.0	5.848361232921985	0.0
21	8.070656987796763E-5	0.0	0.0	6.029305362588389	0.0
22	8.070656987796763E-5	0.0	0.0	6.232363092401355	0.0
23	8.070656987796763E-5	0.0	0.0	6.459431026753017	0.0
24	8.070656987796763E-5	0.0	0.0	6.6261708001208985	0.0
25	8.070656987796763E-5	0.0	0.0	6.774307709131908	0.0
26	8.070656987796763E-5	0.0	0.0	6.9138897217358535	0.0
27	8.070656987796763E-5	0.0	0.0	7.066223372380517	0.0
28	8.070656987796763E-5	0.0	0.0	7.225820614314198	0.0
29	8.070656987796763E-5	0.0	0.0	7.40163987679335	0.0
30	8.070656987796763E-5	0.0	0.0	7.630483355682328	0.0
31	8.070656987796763E-5	0.0	0.0	7.8188524897775045	0.0
32	8.070656987796763E-5	0.0	0.0	7.993824333272938	0.0
33	8.070656987796763E-5	0.0	0.0	8.171782319853856	0.0
34	8.070656987796763E-5	0.0	0.0	8.359546154674948	0.0
35	8.070656987796763E-5	0.0	0.0	8.575274815958755	0.0
36	8.070656987796763E-5	0.0	0.0	8.77445863041758	0.0
37	8.070656987796763E-5	0.0	0.0	8.97816201278957	0.0
38	8.070656987796763E-5	0.0	0.0	9.183358466704302	0.0
39	8.070656987796763E-5	0.0	0.0	9.461715426213413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	100	0.0	45.0	6
TAATTCG	20	7.034868E-4	45.0	18
AGTCGTA	20	7.034868E-4	45.0	36
CGTTTTT	17185	0.0	43.481236	1
CGACGGT	225	0.0	43.0	28
TTCGTAG	185	0.0	42.567566	1
AGGGCGA	1550	0.0	41.80645	6
TACGGGA	1170	0.0	40.576923	4
CGGTCCG	50	1.0822987E-9	40.5	29
CGGTCTA	245	0.0	40.408165	31
CGGTTTT	1575	0.0	40.142857	1
ACGGGAT	1245	0.0	40.120483	5
TCTTGCG	45	1.9297659E-8	40.0	1
ACGCCGA	125	0.0	39.6	12
GGGCGAT	2950	0.0	39.584747	7
CGTTCGA	40	3.4603727E-7	39.375	14
TAGGGCG	650	0.0	38.76923	5
AATGCGG	360	0.0	38.75	2
TAGCGCG	105	0.0	38.57143	1
CGAAAGG	240	0.0	38.437504	2
>>END_MODULE