FastQCFastQC Report
Thu 26 May 2016
SRR1548049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548049_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2169055
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.28127456426877145No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58390.2691955713432808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC302.1663964E-645.0000049
ACGTACT302.1663964E-645.00000410
GCCCGTA302.1663964E-645.00000425
CGTTATG253.891954E-545.029
TCGTCTA502.1827873E-1145.020
CGCGTAA351.2125929E-745.031
TGGTCGA502.1827873E-1145.019
CCCGATA207.034673E-445.017
GTTAGCG1300.043.269231
TTACGCG1050.042.8571431
AAACGCG800.042.18751
TATCGTG750.042.0000041
TACGGGA7100.041.1971864
CGGTCTA3550.041.19718631
CGTTCGA556.184564E-1140.90909222
ACGGTCT3600.040.62530
ATAGTAG3000.040.5000041
CGCAAGG2400.040.3125042
CGTTTTT41300.040.1513331
CGACGGC451.929584E-840.032