##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548049_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2169055
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.541161473544932	31.0	31.0	33.0	30.0	34.0
2	31.94779201080655	31.0	31.0	34.0	30.0	34.0
3	32.03813273522341	33.0	31.0	34.0	30.0	34.0
4	35.74693726069648	37.0	35.0	37.0	35.0	37.0
5	35.64437508500245	37.0	35.0	37.0	33.0	37.0
6	35.704757140782505	37.0	35.0	37.0	33.0	37.0
7	36.0477028014504	37.0	35.0	37.0	35.0	37.0
8	36.070192318774765	37.0	35.0	37.0	35.0	37.0
9	37.88445936133478	39.0	38.0	39.0	35.0	39.0
10	37.35277113766133	39.0	37.0	39.0	34.0	39.0
11	36.98773428981745	39.0	37.0	39.0	33.0	39.0
12	35.99465066584296	37.0	35.0	39.0	32.0	39.0
13	35.56044268125981	37.0	35.0	39.0	31.0	39.0
14	36.5350145570306	38.0	35.0	41.0	31.0	41.0
15	36.84802782778676	38.0	35.0	41.0	32.0	41.0
16	37.033890334731026	38.0	35.0	41.0	32.0	41.0
17	36.970589496347486	38.0	35.0	41.0	32.0	41.0
18	36.924799970494064	38.0	35.0	41.0	32.0	41.0
19	36.85326789777115	38.0	35.0	41.0	32.0	41.0
20	36.724172969334575	38.0	35.0	41.0	32.0	41.0
21	36.564668023632414	38.0	35.0	40.0	32.0	41.0
22	36.43986344283571	38.0	35.0	40.0	31.0	41.0
23	36.38652316331306	38.0	35.0	40.0	31.0	41.0
24	36.400062700115946	38.0	35.0	40.0	31.0	41.0
25	36.359056363254965	37.0	35.0	40.0	31.0	41.0
26	36.21205778553333	37.0	35.0	40.0	31.0	41.0
27	36.14590086466226	37.0	35.0	40.0	31.0	41.0
28	36.100696847244535	37.0	35.0	40.0	31.0	41.0
29	36.08547454997683	37.0	35.0	40.0	31.0	41.0
30	36.04983230024135	37.0	35.0	40.0	31.0	41.0
31	35.938329364631144	37.0	35.0	40.0	30.0	41.0
32	35.7800249417373	37.0	35.0	40.0	30.0	41.0
33	35.70161982983373	37.0	35.0	40.0	30.0	41.0
34	35.606329945529275	37.0	35.0	40.0	30.0	41.0
35	35.496957891800804	37.0	34.0	40.0	29.0	41.0
36	35.31730454045656	36.0	34.0	40.0	29.0	41.0
37	35.200598878313365	36.0	34.0	40.0	28.0	41.0
38	35.135847638718246	36.0	34.0	40.0	28.0	41.0
39	35.134245558549694	36.0	34.0	40.0	27.0	41.0
40	34.93300907538075	36.0	34.0	40.0	27.0	41.0
41	34.95788811256515	36.0	34.0	40.0	27.0	41.0
42	34.90417762574024	36.0	34.0	40.0	27.0	41.0
43	34.79086606840306	36.0	34.0	40.0	27.0	41.0
44	34.61443808478808	35.0	34.0	40.0	26.0	41.0
45	34.591156056439324	35.0	34.0	40.0	26.0	41.0
46	34.51733865669612	35.0	34.0	40.0	26.0	41.0
47	34.46423949600172	35.0	33.0	40.0	26.0	41.0
48	34.365841345655134	35.0	34.0	40.0	26.0	41.0
49	34.28705081245058	35.0	34.0	40.0	25.0	41.0
50	34.113309713216125	35.0	33.0	39.0	24.0	41.0
51	33.71012445511985	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	8.0
12	19.0
13	18.0
14	20.0
15	64.0
16	181.0
17	414.0
18	913.0
19	1781.0
20	3088.0
21	5089.0
22	7498.0
23	11129.0
24	15698.0
25	21755.0
26	29011.0
27	34645.0
28	39211.0
29	45152.0
30	54890.0
31	67409.0
32	84153.0
33	112311.0
34	212808.0
35	358629.0
36	135416.0
37	171263.0
38	264103.0
39	492244.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.57432384148857	24.43829225169486	30.756804230413703	12.230579676402858
2	29.57735050517391	26.237785579434362	30.62660928376643	13.558254631625294
3	29.28574886298411	25.894225826454374	30.892347128127223	13.927678182434287
4	26.851462964286288	28.09343239336946	29.507781038286257	15.547323604057988
5	23.573906609099353	33.68646714813594	28.17365165936318	14.565974583401529
6	22.43631443186088	41.03035653775492	25.584597900929207	10.948731129454993
7	88.84076245185115	4.401916963839091	5.192307249009361	1.5650133353003959
8	90.21721440903988	2.9409581591983605	4.732291251259189	2.109536180502569
9	85.5508043825537	5.229005258050165	6.383194524804581	2.8369958345915616
10	51.84783235095468	28.0638803534258	10.803183875005475	9.285103420614046
11	45.802711318984535	18.661536936592203	21.30231829068419	14.23343345373907
12	43.01744308005099	19.256404286659397	23.161791655813246	14.564360977476367
13	22.775817118514745	38.34268840578039	23.14989707499349	15.731597400711369
14	15.63473494217528	41.830244046370424	25.652461555838833	16.882559455615464
15	14.054323196046203	24.314136801510337	47.12130397799964	14.510236024443824
16	16.75324046647042	19.464605554031593	46.01713649492521	17.765017484572777
17	17.315697388955098	19.672299688113025	27.51327190873445	35.498731014197425
18	21.729601139666812	24.28702822196763	32.90243908061345	21.08093155775211
19	27.849547383537992	24.980187224390345	25.95051762172928	21.219747770342384
20	31.23982563835403	23.09263711616349	25.055519569582145	20.612017675900336
21	23.24316349746779	27.480538759966898	27.718799200573525	21.55749854199179
22	23.008960123187286	23.428082736491238	25.924653823900268	27.638303316421208
23	19.767456334671092	29.535581163225462	25.171975814352333	25.524986687751117
24	20.612294294058934	23.723418723822125	37.11367392712495	18.550613054993995
25	17.40776513274214	25.623416649185938	34.15275315748102	22.816065060590905
26	17.735649856734845	34.32434862186528	26.712554545643147	21.227446975756724
27	19.35663226612511	33.40205757806971	27.454029519767825	19.78728063603735
28	15.747779562989411	28.635650087249974	37.735373238576244	17.881197111184363
29	16.529225861031644	24.236729820129042	36.93686882075374	22.297175498085572
30	18.842168594157364	29.641110990730983	31.150201354967948	20.366519060143702
31	28.67857200485926	25.424343781047508	24.789781725221353	21.10730248887188
32	28.25778968260371	27.66412101122378	26.393475499699175	17.684613806473326
33	27.154359847952218	28.01090797605409	24.941368476133615	19.89336369986008
34	19.353451157301222	28.853809608331737	28.19559669994537	23.59714253442167
35	20.028998803626465	26.26669217700796	31.02825885005221	22.676050169313363
36	29.773288367514883	24.09325720186902	26.895076427292068	19.238378003324026
37	20.55959853484582	31.226271348582678	28.275493244754053	19.93863687181745
38	20.796107060447984	31.580711415800888	24.294911839487703	23.32826968426342
39	21.06378123191897	29.720915329486804	27.727420466516524	21.487882972077703
40	23.425132142799516	26.764604862486195	25.71221107809622	24.098051916618065
41	17.301221038655083	24.99480188376966	27.836915154295305	29.86706192327995
42	22.985401476679936	25.94664496750889	24.746629292479906	26.321324263331263
43	23.8249836910544	26.34391474628352	24.62150567874028	25.2095958839218
44	20.172240906754325	30.914845405026615	27.533833858523643	21.37907982969542
45	18.371180076116097	35.45599350869388	23.16211437699828	23.01071203819175
46	22.347058972686263	31.546226352028878	26.224046877557278	19.88266779772758
47	22.06241888748787	27.14813593938374	27.345779613702742	23.443665559425646
48	23.682479236349472	23.966981012468562	29.65259064431285	22.69794910686912
49	20.795830442289382	23.48760174361646	31.435809603721438	24.280758210372717
50	19.506789823217947	30.682440048777003	27.804781344871383	22.005988783133667
51	18.934236337944405	32.87634476765227	24.116354818112036	24.07306407629129
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	842.0
1	2008.5
2	3175.0
3	7305.0
4	11435.0
5	8939.0
6	6443.0
7	6384.0
8	6325.0
9	6556.0
10	6787.0
11	6692.5
12	6598.0
13	6571.5
14	6545.0
15	6460.5
16	6376.0
17	5986.0
18	5596.0
19	5297.5
20	4999.0
21	5674.5
22	6350.0
23	7151.0
24	7952.0
25	9022.0
26	14022.0
27	17952.0
28	22401.0
29	26850.0
30	30736.5
31	34623.0
32	36406.0
33	38189.0
34	46255.0
35	54321.0
36	59728.5
37	65136.0
38	73586.0
39	82036.0
40	118812.5
41	155589.0
42	182944.0
43	210299.0
44	218149.5
45	226000.0
46	217548.0
47	209096.0
48	195398.5
49	181701.0
50	171078.0
51	160455.0
52	144347.5
53	128240.0
54	116147.0
55	104054.0
56	97554.5
57	91055.0
58	86193.5
59	81332.0
60	74505.5
61	67679.0
62	58782.0
63	49885.0
64	41731.0
65	33577.0
66	28285.0
67	22993.0
68	18987.0
69	14981.0
70	11944.0
71	8907.0
72	7771.5
73	6636.0
74	5190.0
75	2746.5
76	1749.0
77	1420.0
78	1091.0
79	929.5
80	768.0
81	480.5
82	193.0
83	151.0
84	109.0
85	97.0
86	85.0
87	76.5
88	68.0
89	67.5
90	67.0
91	63.0
92	59.0
93	33.5
94	8.0
95	10.5
96	13.0
97	6.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2169055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.518300520871904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6455863908344	15.119375049882805
2	7.995669221863016	2.9613241103189063
3	2.470228510042092	1.372333017125553
4	1.1052786354792359	0.8187152772441487
5	0.6316708988362144	0.5848735767469146
6	0.4131917321122466	0.4590965200796507
7	0.2745300700601571	0.35586812375730054
8	0.2376518297483512	0.35207264020920415
9	0.1699857729328948	0.2833062864699635
>10	2.048951385618924	9.591389033413371
>50	1.3184515537863362	18.17293664894178
>100	1.6810777702404938	48.46222051199634
>500	0.006729295743011407	0.7721522118653598
>1k	4.984663513341784E-4	0.14326123524387785
>5k	4.984663513341784E-4	0.5510757567048652
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6101	0.28127456426877145	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5839	0.2691955713432808	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.22060528663404E-5	0.0	0.0	0.0403862511554571	0.0
2	9.22060528663404E-5	0.0	0.0	0.14232004259919642	0.0
3	9.22060528663404E-5	0.0	0.0	0.20801685526646396	0.0
4	9.22060528663404E-5	0.0	0.0	0.3473402011475043	0.0
5	9.22060528663404E-5	0.0	0.0	0.6132624576140301	0.0
6	9.22060528663404E-5	0.0	0.0	0.9048640998038316	0.0
7	9.22060528663404E-5	0.0	0.0	1.089183999483646	0.0
8	9.22060528663404E-5	0.0	0.0	1.6282666875667053	0.0
9	9.22060528663404E-5	0.0	0.0	1.8313966220312532	0.0
10	9.22060528663404E-5	0.0	0.0	2.1883262526768568	0.0
11	9.22060528663404E-5	0.0	0.0	2.519161570361286	0.0
12	9.22060528663404E-5	0.0	0.0	2.8168949150666998	0.0
13	9.22060528663404E-5	0.0	0.0	2.937869256427338	0.0
14	9.22060528663404E-5	0.0	0.0	2.97890094995286	0.0
15	9.22060528663404E-5	0.0	0.0	3.0487470349991126	0.0
16	9.22060528663404E-5	0.0	0.0	3.2218177962292334	0.0
17	9.22060528663404E-5	0.0	0.0	3.4158193314600136	0.0
18	9.22060528663404E-5	0.0	0.0	3.707651488781981	0.0
19	9.22060528663404E-5	0.0	0.0	3.8302855390942137	0.0
20	9.22060528663404E-5	0.0	0.0	3.977446399468893	0.0
21	9.22060528663404E-5	0.0	0.0	4.156879378346791	0.0
22	9.22060528663404E-5	0.0	0.0	4.33456044222023	0.0
23	9.22060528663404E-5	0.0	0.0	4.538566334187007	0.0
24	9.22060528663404E-5	0.0	0.0	4.690752424442903	0.0
25	9.22060528663404E-5	0.0	0.0	4.812187796067873	0.0
26	9.22060528663404E-5	0.0	0.0	4.932332282952714	0.0
27	9.22060528663404E-5	0.0	0.0	5.045976243110479	0.0
28	9.22060528663404E-5	0.0	0.0	5.16713499657685	0.0
29	9.22060528663404E-5	0.0	0.0	5.299496785466482	0.0
30	9.22060528663404E-5	0.0	0.0	5.468648789449784	0.0
31	1.3830907929951062E-4	0.0	0.0	5.63992153264901	0.0
32	1.3830907929951062E-4	0.0	0.0	5.788234968684519	0.0
33	1.3830907929951062E-4	0.0	0.0	5.92479213297957	0.0
34	1.3830907929951062E-4	0.0	0.0	6.079513889689289	0.0
35	1.3830907929951062E-4	0.0	0.0	6.2796010244092475	0.0
36	1.3830907929951062E-4	0.0	0.0	6.454239288538096	0.0
37	1.3830907929951062E-4	0.0	0.0	6.634548224918225	0.0
38	1.844121057326808E-4	0.0	0.0	6.796369847698652	0.0
39	2.3051513216585103E-4	0.0	0.0	6.964968615364755	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGAC	30	2.1663964E-6	45.000004	9
ACGTACT	30	2.1663964E-6	45.000004	10
GCCCGTA	30	2.1663964E-6	45.000004	25
CGTTATG	25	3.891954E-5	45.0	29
TCGTCTA	50	2.1827873E-11	45.0	20
CGCGTAA	35	1.2125929E-7	45.0	31
TGGTCGA	50	2.1827873E-11	45.0	19
CCCGATA	20	7.034673E-4	45.0	17
GTTAGCG	130	0.0	43.26923	1
TTACGCG	105	0.0	42.857143	1
AAACGCG	80	0.0	42.1875	1
TATCGTG	75	0.0	42.000004	1
TACGGGA	710	0.0	41.197186	4
CGGTCTA	355	0.0	41.197186	31
CGTTCGA	55	6.184564E-11	40.909092	22
ACGGTCT	360	0.0	40.625	30
ATAGTAG	300	0.0	40.500004	1
CGCAAGG	240	0.0	40.312504	2
CGTTTTT	4130	0.0	40.151333	1
CGACGGC	45	1.929584E-8	40.0	32
>>END_MODULE