FastQCFastQC Report
Thu 26 May 2016
SRR1548048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548048_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3756070
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117160.3119217692960994No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC83430.2221204610137724No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT207.035332E-445.00000416
GGCGATA12100.041.2809948
CGTTAGG6800.041.029412
CGGTCTA8550.040.52631831
TACGGGA15750.040.04
TATACGG4050.040.02
CGTTTTT70750.039.911661
TCGATAG2050.039.5121961
CGAACTA403.4610093E-739.37500434
CGACGGT8700.039.31034528
TAACGGG11400.039.0789453
GTTTACG1850.038.918921
AGGGCGA26200.038.902676
GGGCGAT47100.038.31217
CGTAAGG5350.038.2710272
TAGTACG2650.038.2075421
ACGGTAC659.094947E-1238.07692726
TAAGGGA34900.037.8438384
GTAGGGA46050.037.768734
GGCGATT14250.037.736848
Produced by FastQC (version 0.11.5)