##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548048_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3756070
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51972433953574	31.0	31.0	33.0	30.0	34.0
2	31.92187153061578	31.0	31.0	34.0	30.0	34.0
3	32.008968682692284	33.0	31.0	34.0	30.0	34.0
4	35.724226918028684	37.0	35.0	37.0	35.0	37.0
5	35.62278338795603	37.0	35.0	37.0	33.0	37.0
6	35.6840500842636	37.0	35.0	37.0	33.0	37.0
7	36.04949002547876	37.0	35.0	37.0	35.0	37.0
8	36.06851070400712	37.0	35.0	37.0	35.0	37.0
9	37.87528374071836	39.0	38.0	39.0	35.0	39.0
10	37.32115801888676	39.0	37.0	39.0	34.0	39.0
11	36.98415125383712	39.0	37.0	39.0	33.0	39.0
12	36.18927096672852	38.0	35.0	39.0	32.0	39.0
13	35.85557564156153	38.0	35.0	39.0	31.0	39.0
14	36.80853232234756	39.0	35.0	41.0	31.0	41.0
15	37.0770392990546	39.0	35.0	41.0	32.0	41.0
16	37.2391771186373	39.0	35.0	41.0	32.0	41.0
17	37.187437401326385	39.0	35.0	41.0	32.0	41.0
18	37.12871245743556	38.0	35.0	41.0	32.0	41.0
19	37.080977457821604	38.0	35.0	41.0	32.0	41.0
20	36.9633316205502	38.0	35.0	41.0	32.0	41.0
21	36.8118195347797	38.0	35.0	41.0	32.0	41.0
22	36.724674726509356	38.0	35.0	41.0	31.0	41.0
23	36.64422308423432	38.0	35.0	41.0	31.0	41.0
24	36.65881093802831	38.0	35.0	41.0	31.0	41.0
25	36.63048505485787	38.0	35.0	41.0	31.0	41.0
26	36.50284233254439	38.0	35.0	40.0	31.0	41.0
27	36.429925693610606	38.0	35.0	40.0	31.0	41.0
28	36.38047214242546	38.0	35.0	40.0	31.0	41.0
29	36.34172925424713	38.0	35.0	40.0	31.0	41.0
30	36.253933233406194	38.0	35.0	40.0	30.0	41.0
31	36.15513076167377	38.0	35.0	40.0	30.0	41.0
32	36.03396768430833	38.0	35.0	40.0	30.0	41.0
33	35.923103137055485	38.0	35.0	40.0	30.0	41.0
34	35.80653076220624	38.0	35.0	40.0	30.0	41.0
35	35.687893729350094	38.0	35.0	40.0	29.0	41.0
36	35.50042757456597	38.0	34.0	40.0	28.0	41.0
37	35.396133991113054	38.0	34.0	40.0	27.0	41.0
38	35.27482927634469	37.0	34.0	40.0	27.0	41.0
39	35.251434078704605	37.0	34.0	40.0	27.0	41.0
40	35.095350459389735	37.0	34.0	40.0	26.0	41.0
41	35.04543791782368	37.0	34.0	40.0	26.0	41.0
42	34.995061327398055	37.0	34.0	40.0	26.0	41.0
43	34.868172850878715	37.0	34.0	40.0	26.0	41.0
44	34.721080544292306	36.0	34.0	40.0	26.0	41.0
45	34.66503978892832	36.0	34.0	40.0	25.0	41.0
46	34.609651843549244	36.0	34.0	40.0	25.0	41.0
47	34.52173495169153	36.0	33.0	40.0	25.0	41.0
48	34.39289443487475	36.0	33.0	40.0	24.0	41.0
49	34.31271195691241	36.0	33.0	40.0	24.0	41.0
50	34.13706214207935	35.0	33.0	40.0	24.0	41.0
51	33.73660421664133	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	16.0
11	14.0
12	19.0
13	30.0
14	65.0
15	166.0
16	282.0
17	754.0
18	1539.0
19	3250.0
20	5617.0
21	9243.0
22	13427.0
23	19978.0
24	28404.0
25	38585.0
26	51125.0
27	60741.0
28	68468.0
29	78248.0
30	92336.0
31	113722.0
32	141109.0
33	184674.0
34	327396.0
35	520309.0
36	249769.0
37	322819.0
38	503102.0
39	920716.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.1398057011717	23.390645009278316	30.063710207743732	12.40583908180625
2	30.50054977676135	25.784024259398784	30.333806345462143	13.38161961837772
3	29.838394918092582	25.45487703903282	30.219750963107717	14.486977079766882
4	26.64542993075209	27.84554068481152	30.01549491889129	15.493534465545103
5	23.68148090956771	32.8066835815094	28.449097061556362	15.062738447366531
6	21.86178638843259	41.67201356737228	25.75449871807501	10.711701326120119
7	89.0847082189629	3.9088195906892045	5.268618529473625	1.737853660874265
8	89.9327222336112	2.9453391443716437	5.033053164610883	2.0888854574062785
9	85.34404310888775	5.002249691832154	6.674955472075866	2.9787517272042323
10	48.276256832274164	28.899780888002617	12.267849108243457	10.556113171479765
11	41.42814164805235	20.329759562521467	23.06349455681071	15.178604232615472
12	37.410271906540615	21.03954399145916	25.26039184573238	16.289792256267855
13	24.416451237596743	33.70009078638044	25.93969228475508	15.943765691267734
14	17.63779695266595	36.8620126887944	28.654311554364003	16.84587880417564
15	16.34490305026264	25.256664545655433	42.509697636093044	15.888734767988883
16	19.861450931425665	21.960160486891883	40.259366838211214	17.919021743471234
17	20.72442739352568	21.149179860865214	27.742134731248353	30.384258014360753
18	23.013229252915945	23.616386276081116	32.78056585739883	20.589818613604113
19	27.212538637458835	25.414808563205693	26.692660147441345	20.679992651894132
20	29.17645304799964	25.160367085810435	25.397210382128126	20.2659694840618
21	24.22776998298754	26.397058627767855	29.345885460068637	20.029285929175973
22	24.2462201183684	23.220680125769753	27.484525048787695	25.04857470707415
23	20.474032699071103	28.322235741080437	27.171964313764118	24.03176724608434
24	20.821177454094304	25.165132705194527	34.489133589097115	19.524556251614055
25	19.379111677897377	25.920123959351134	32.580915691134614	22.119848671616875
26	18.630723069591355	32.30320521183045	26.93623920746951	22.129832511108685
27	19.449531025779603	31.04856938235975	29.70426536246662	19.79763422939402
28	17.19829502645052	27.891998817913404	35.80242114763569	19.10728500800038
29	18.51357935288746	24.79818001261957	34.8397127849055	21.84852784958747
30	20.29198071388446	28.611953451346757	31.241616902773377	19.85444893199541
31	26.92117026572987	26.257950464181977	26.050739203475974	20.77014006661218
32	28.39172326394343	26.93437555743104	26.546922714432903	18.12697846419263
33	26.31069176027071	27.537106603444556	26.398443053510718	19.75375858277402
34	20.428506390988453	28.02013806984428	29.128397500579062	22.422958038588206
35	21.27862366782302	27.03889970101729	29.89811159003959	21.784365041120108
36	27.971629921700075	25.15927551935933	28.02114976557945	18.847944793361147
37	21.904943198609182	29.56347991384612	29.04573130958688	19.48584557795781
38	21.415096097783053	30.60374274174869	25.633947184157908	22.347213976310346
39	21.01214833589363	29.538666744762477	27.9486804026549	21.500504516688988
40	23.967178460465327	26.003934963938374	26.83347221963382	23.19541435596248
41	19.96272699923058	24.253435106374482	28.85428120349195	26.929556690902988
42	23.212985913468067	25.0949529694601	26.51319064873658	25.17887046833526
43	22.827130484788622	26.276187610987016	27.22180364050723	23.67487826371713
44	21.068324072767545	29.043734541688522	27.896498201577714	21.99144318396622
45	19.340267886381245	32.98857582526417	25.02687649591195	22.644279792442635
46	22.622794569856257	30.35225648084301	26.869547159664226	20.15540178963651
47	22.05723003032425	28.01997832841241	27.856589467182452	22.06620217408089
48	22.682617736091178	25.989824470789948	29.868133447992186	21.45942434512669
49	22.39500328801114	24.335355837351276	30.465113802458422	22.804527072179166
50	20.761114675711582	29.403578740545306	28.031027110783345	21.80427947295977
51	19.64010255399926	31.315097961433093	25.938920201167708	23.105879283399936
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1665.0
1	3252.0
2	4839.0
3	13066.0
4	21293.0
5	16134.5
6	10976.0
7	10808.0
8	10640.0
9	11142.0
10	11644.0
11	11844.0
12	12044.0
13	11561.5
14	11079.0
15	10755.0
16	10431.0
17	10227.0
18	10023.0
19	9945.5
20	9868.0
21	11909.5
22	13951.0
23	14403.0
24	14855.0
25	17407.0
26	24741.0
27	29523.0
28	37554.0
29	45585.0
30	53242.5
31	60900.0
32	68157.0
33	75414.0
34	85926.0
35	96438.0
36	110827.5
37	125217.0
38	140867.5
39	156518.0
40	201579.5
41	246641.0
42	291063.5
43	335486.0
44	354289.0
45	373092.0
46	366172.0
47	359252.0
48	340970.0
49	322688.0
50	299534.5
51	276381.0
52	254715.0
53	233049.0
54	212378.5
55	191708.0
56	177719.0
57	163730.0
58	148738.0
59	133746.0
60	123539.5
61	113333.0
62	97453.0
63	81573.0
64	70535.5
65	59498.0
66	51278.5
67	43059.0
68	33845.0
69	24631.0
70	22210.5
71	19790.0
72	15636.5
73	11483.0
74	8820.0
75	4740.0
76	3323.0
77	2670.5
78	2018.0
79	1726.0
80	1434.0
81	990.5
82	547.0
83	393.0
84	239.0
85	198.0
86	157.0
87	118.0
88	79.0
89	71.5
90	64.0
91	42.0
92	20.0
93	19.0
94	18.0
95	13.5
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3756070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.944671969977815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07449711826582	15.359297530364577
2	8.358505103552988	3.166982746923936
3	2.76141348265747	1.5694221780725937
4	1.2840889070177242	0.9730657249495251
5	0.7214112688141667	0.6833449921564937
6	0.4678387585885817	0.5317831089781394
7	0.3470569392827685	0.460241590873159
8	0.2426461127737886	0.3677480809031735
9	0.19737258949149625	0.3365243067403338
>10	1.9503362641701187	8.63578344782157
>50	0.9606726196186626	13.496583894503845
>100	1.6243959467244578	52.57803643148875
>500	0.008208167600537629	0.9963863361497254
>1k	0.0012736811793937698	0.30700731716851004
>5k	1.415201310437522E-4	0.22368020671879127
>10k+	1.415201310437522E-4	0.3141121061869062
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11716	0.3119217692960994	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8343	0.2221204610137724	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6623571978158023E-5	0.0	0.0	0.042943821600768886	0.0
2	2.6623571978158023E-5	0.0	0.0	0.15300566815847416	0.0
3	2.6623571978158023E-5	0.0	0.0	0.23665693131384666	0.0
4	2.6623571978158023E-5	0.0	0.0	0.366952692574952	0.0
5	2.6623571978158023E-5	0.0	0.0	0.6436248525719702	0.0
6	2.6623571978158023E-5	0.0	0.0	0.9388536422377645	0.0
7	2.6623571978158023E-5	0.0	0.0	1.1179770345068116	0.0
8	2.6623571978158023E-5	0.0	0.0	1.609820903231303	0.0
9	2.6623571978158023E-5	0.0	0.0	1.796478766370169	0.0
10	2.6623571978158023E-5	0.0	0.0	2.1488683650730684	0.0
11	2.6623571978158023E-5	0.0	0.0	2.512865841158445	0.0
12	2.6623571978158023E-5	0.0	0.0	2.81256739091657	0.0
13	2.6623571978158023E-5	0.0	0.0	2.9410793728551385	0.0
14	2.6623571978158023E-5	0.0	0.0	2.9936609275120007	0.0
15	2.6623571978158023E-5	0.0	0.0	3.077099202091548	0.0
16	2.6623571978158023E-5	0.0	0.0	3.27422012901783	0.0
17	2.6623571978158023E-5	0.0	0.0	3.5016120572832774	0.0
18	2.6623571978158023E-5	0.0	0.0	3.8068513100128594	0.0
19	2.6623571978158023E-5	0.0	0.0	3.956076430950435	0.0
20	2.6623571978158023E-5	0.0	0.0	4.129635496676047	0.0
21	2.6623571978158023E-5	0.0	0.0	4.344540969683739	0.0
22	2.6623571978158023E-5	0.0	0.0	4.568604951451917	0.0
23	2.6623571978158023E-5	0.0	0.0	4.8099210078619405	0.0
24	2.6623571978158023E-5	0.0	0.0	4.9876067272441675	0.0
25	2.6623571978158023E-5	0.0	0.0	5.1439137183279335	0.0
26	2.6623571978158023E-5	0.0	0.0	5.297371987210036	0.0
27	2.6623571978158023E-5	0.0	0.0	5.4655797149680385	0.0
28	2.6623571978158023E-5	0.0	0.0	5.632056910547461	0.0
29	2.6623571978158023E-5	0.0	0.0	5.814215390022017	0.0
30	2.6623571978158023E-5	0.0	0.0	6.0352975317286415	0.0
31	2.6623571978158023E-5	0.0	0.0	6.23247170579888	0.0
32	2.6623571978158023E-5	0.0	0.0	6.414177584549809	0.0
33	2.6623571978158023E-5	0.0	0.0	6.605601067072765	0.0
34	2.6623571978158023E-5	0.0	0.0	6.814702601389218	0.0
35	2.6623571978158023E-5	0.0	0.0	7.062754421509716	0.0
36	2.6623571978158023E-5	0.0	0.0	7.2762488452025655	0.0
37	2.6623571978158023E-5	0.0	0.0	7.486415322398145	0.0
38	2.6623571978158023E-5	0.0	0.0	7.725761234481785	0.0
39	2.6623571978158023E-5	0.0	0.0	8.058689002068652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTT	20	7.035332E-4	45.000004	16
GGCGATA	1210	0.0	41.280994	8
CGTTAGG	680	0.0	41.02941	2
CGGTCTA	855	0.0	40.526318	31
TACGGGA	1575	0.0	40.0	4
TATACGG	405	0.0	40.0	2
CGTTTTT	7075	0.0	39.91166	1
TCGATAG	205	0.0	39.512196	1
CGAACTA	40	3.4610093E-7	39.375004	34
CGACGGT	870	0.0	39.310345	28
TAACGGG	1140	0.0	39.078945	3
GTTTACG	185	0.0	38.91892	1
AGGGCGA	2620	0.0	38.90267	6
GGGCGAT	4710	0.0	38.3121	7
CGTAAGG	535	0.0	38.271027	2
TAGTACG	265	0.0	38.207542	1
ACGGTAC	65	9.094947E-12	38.076927	26
TAAGGGA	3490	0.0	37.843838	4
GTAGGGA	4605	0.0	37.76873	4
GGCGATT	1425	0.0	37.73684	8
>>END_MODULE