FastQCFastQC Report
Thu 26 May 2016
SRR1548047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548047_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences120892
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4090.3383184991562717No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.24650100916520531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAA207.0082274E-445.00000429
GTTGATC207.0082274E-445.00000416
TTTAGAG302.149358E-645.0000041
TGTCAAC207.0082274E-445.00000413
CGAACTA207.0082274E-445.00000436
CATATGC207.0082274E-445.00000433
CTCAAAG207.0082274E-445.00000444
GGCACCG351.1998964E-745.0000048
TGAACGG207.0082274E-445.0000042
GCACGGG302.149358E-645.0000043
ATAGAAC406.728442E-945.00000437
GCACCGA351.1998964E-745.0000049
ATCCGGA207.0082274E-445.00000428
AGCGGGT207.0082274E-445.0000044
GTAGCAT207.0082274E-445.00000429
TAGGGTG207.0082274E-445.0000045
TGTTAAG207.0082274E-445.0000041
GAACGGG406.728442E-945.0000043
GAACGAA207.0082274E-445.00000433
ACTAACC406.728442E-945.00000414