##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548047_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	120892
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56810210766635	31.0	31.0	33.0	30.0	34.0
2	31.979849783277636	31.0	31.0	34.0	30.0	34.0
3	32.077060516824936	33.0	31.0	34.0	30.0	34.0
4	35.769471925354864	37.0	35.0	37.0	35.0	37.0
5	35.673452337623665	37.0	35.0	37.0	33.0	37.0
6	35.7408926976144	37.0	35.0	37.0	33.0	37.0
7	36.06981437977699	37.0	35.0	37.0	35.0	37.0
8	36.09581279158257	37.0	35.0	37.0	35.0	37.0
9	37.96012970254442	39.0	38.0	39.0	35.0	39.0
10	37.419150977732194	39.0	37.0	39.0	35.0	39.0
11	37.03128412136452	39.0	37.0	39.0	33.0	39.0
12	35.66018429672766	37.0	35.0	39.0	32.0	39.0
13	35.06812692320418	35.0	35.0	39.0	30.0	39.0
14	35.92073090030771	37.0	35.0	40.0	31.0	41.0
15	36.33095655626509	37.0	35.0	40.0	31.0	41.0
16	36.60472984151143	37.0	35.0	40.0	32.0	41.0
17	36.584207391721534	37.0	35.0	40.0	32.0	41.0
18	36.55367600833802	36.0	35.0	40.0	32.0	41.0
19	36.471553121794656	36.0	35.0	40.0	32.0	41.0
20	36.333413294510805	36.0	35.0	40.0	32.0	41.0
21	36.14536942063991	36.0	35.0	40.0	31.0	41.0
22	36.03408000529398	35.0	35.0	40.0	31.0	41.0
23	36.02646990702445	35.0	35.0	40.0	31.0	41.0
24	36.035420044337094	35.0	35.0	40.0	31.0	41.0
25	35.98710419217152	35.0	35.0	40.0	31.0	41.0
26	35.82862389570857	35.0	35.0	40.0	31.0	41.0
27	35.76564206068226	35.0	35.0	40.0	31.0	41.0
28	35.76453363332561	36.0	35.0	40.0	31.0	41.0
29	35.77512986798134	36.0	35.0	40.0	31.0	41.0
30	35.766113555901136	36.0	35.0	40.0	31.0	41.0
31	35.62300234920425	36.0	34.0	40.0	30.0	41.0
32	35.48752605631473	35.0	34.0	40.0	30.0	41.0
33	35.39767726565861	35.0	34.0	40.0	30.0	41.0
34	35.322941137544255	35.0	34.0	40.0	30.0	41.0
35	35.22495285047811	35.0	34.0	40.0	30.0	41.0
36	34.99972702908381	35.0	34.0	40.0	29.0	41.0
37	34.856136055322104	35.0	34.0	40.0	27.0	41.0
38	34.83369453727294	35.0	34.0	40.0	28.0	41.0
39	34.872911358898854	35.0	34.0	40.0	28.0	41.0
40	34.70143599245608	35.0	34.0	40.0	27.0	41.0
41	34.725283724315915	35.0	34.0	40.0	27.0	41.0
42	34.66227707375178	35.0	34.0	40.0	27.0	41.0
43	34.5314081990537	35.0	34.0	40.0	27.0	41.0
44	34.41239287959501	35.0	34.0	40.0	27.0	41.0
45	34.3600569103001	35.0	34.0	40.0	27.0	41.0
46	34.3044783773947	35.0	34.0	39.0	27.0	41.0
47	34.232389239982794	35.0	33.0	39.0	27.0	41.0
48	34.174511133904645	35.0	34.0	39.0	26.0	41.0
49	34.17682725076928	35.0	34.0	39.0	26.0	41.0
50	34.00080236905668	35.0	34.0	39.0	26.0	41.0
51	33.55179002746253	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	0.0
13	2.0
14	2.0
15	4.0
16	8.0
17	24.0
18	53.0
19	98.0
20	193.0
21	282.0
22	433.0
23	614.0
24	862.0
25	1223.0
26	1586.0
27	1989.0
28	2181.0
29	2563.0
30	3092.0
31	3813.0
32	4739.0
33	6756.0
34	13882.0
35	25013.0
36	6426.0
37	8373.0
38	12838.0
39	23837.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.473546636667436	25.179499057009565	30.87218343645568	12.47477086986732
2	29.10945306554611	26.674221619296564	31.135228137511167	13.081097177646164
3	28.756245243688582	25.896668100453297	31.184859213182015	14.162227442676107
4	25.45908744995533	28.40386460642557	30.643880488369785	15.493167455249313
5	23.948648380372564	32.512490487377164	29.515600701452538	14.023260430797738
6	21.276842140091983	41.536247228931614	26.06789531151772	11.11901531945869
7	90.03408000529399	3.925818085563974	4.525526916586705	1.5145749925553387
8	91.9639016642954	2.712338285411773	3.9994375144757304	1.324322535817093
9	88.88429341891937	4.170664725540152	5.030936703834827	1.9141051517056547
10	58.22469642325381	25.006617476756116	8.77725573238924	7.991430367600834
11	55.060715349237334	17.539622142077228	16.19461999139728	11.205042517288158
12	49.89329318730768	19.367700095953413	18.110379512291964	12.628627204446943
13	21.875723786520197	46.33474506170797	19.08314859544056	12.706382556331272
14	13.204347682228768	49.40939019951692	24.913145617576017	12.473116500678291
15	11.737749396155246	23.20997253747146	52.847996558912094	12.204281507461205
16	13.737054561095855	17.644674585580518	52.05803527115111	16.560235582172517
17	15.503920854977995	16.750488038910763	27.51298679813387	40.23260430797737
18	22.055222843529762	22.07838401217616	34.496906329616515	21.369486814677565
19	29.539589054693444	23.774112430930085	25.01240776891771	21.673890745458756
20	33.65896833537372	22.22562286999967	22.671475366442774	21.443933428183833
21	22.54574330807663	28.393111206696886	27.77189557621679	21.289249909009694
22	24.70055917678589	22.57965787645171	24.761770836779938	27.958012109982466
23	18.351917413890085	30.525593091354263	23.73688912417695	27.3856003705787
24	19.915296297521756	22.946927836415977	39.45008768156702	17.68768818449525
25	16.15408794626609	23.37706382556331	38.228336035469674	22.240512192700923
26	15.369089766072197	37.86189326009992	25.71965059722728	21.049366376600602
27	17.22446481156735	35.96433180028455	29.213678324454882	17.597525063693215
28	13.696522515964663	29.75713860305066	41.183039407074084	15.363299473910597
29	14.17463521159382	24.433378552757834	40.83893061575621	20.553055619892135
30	18.030142606624093	30.32458723488734	32.72921285113986	18.91605730734871
31	31.008668894550507	25.7502564272243	23.66575124904874	19.575323429176457
32	31.562882572874962	28.236773318333718	24.502034874102506	15.698309234688812
33	29.422128842272443	28.74052873639281	23.018892896138702	18.818449525196044
34	19.487641862157957	29.6893094663005	27.06630711709625	23.75674155444529
35	19.45538166297191	26.73377891010158	31.29239321046885	22.518446216457665
36	32.70605168249347	21.487774211693083	27.790920821890612	18.015253283922842
37	21.592826655196372	30.88293683618436	29.643814313602224	17.88042219501704
38	20.92197994904543	32.55054097872481	22.252919961618634	24.274559110611126
39	20.096449723720344	31.668762200972765	27.57668001191146	20.658108063395428
40	23.865929920921154	26.39215167256725	23.479634715283062	26.262283691228532
41	16.92915991132581	23.123945339641992	28.39228402210237	31.554610726929823
42	23.73027164742084	24.782450451642788	23.937894980643883	27.54938292029249
43	24.038811501174603	26.833041061443268	24.443304767892002	24.684842669490124
44	19.981471065082886	32.26350792442842	26.88101776792509	20.87400324256361
45	16.639645303245874	39.92902756179069	21.058465407140257	22.37286172782318
46	23.896535750918176	32.74989246600271	25.080236905667867	18.273334877411244
47	23.12725407802005	28.720676306124478	25.274625285378683	22.87744433047679
48	24.281176587367238	23.013102603977103	29.926711444926053	22.77900936372961
49	21.587036363034777	21.638321807894652	32.41488270522449	24.35975912384608
50	19.64315256592661	33.496840154848954	25.941336068557057	20.918671210667373
51	17.8018396585382	36.98259603613142	22.12718790325249	23.088376402077888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	110.0
2	168.0
3	361.0
4	554.0
5	455.0
6	356.0
7	343.0
8	330.0
9	347.5
10	365.0
11	362.0
12	359.0
13	337.5
14	316.0
15	310.5
16	305.0
17	267.0
18	229.0
19	246.0
20	263.0
21	239.0
22	215.0
23	261.0
24	307.0
25	428.5
26	565.0
27	580.0
28	757.5
29	935.0
30	1169.5
31	1404.0
32	1660.0
33	1916.0
34	2283.5
35	2651.0
36	2745.0
37	2839.0
38	3851.0
39	4863.0
40	7072.5
41	9282.0
42	11615.5
43	13949.0
44	14402.5
45	14856.0
46	13690.0
47	12524.0
48	11684.0
49	10844.0
50	10067.0
51	9290.0
52	8768.0
53	8246.0
54	6928.5
55	5611.0
56	5004.5
57	4398.0
58	3972.0
59	3546.0
60	3196.5
61	2847.0
62	2501.5
63	2156.0
64	1796.5
65	1437.0
66	1185.5
67	934.0
68	739.5
69	545.0
70	419.5
71	294.0
72	259.0
73	224.0
74	187.5
75	122.5
76	94.0
77	66.5
78	39.0
79	41.0
80	43.0
81	24.5
82	6.0
83	7.0
84	8.0
85	6.5
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	120892.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.394070740826525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.9578706727629	20.35122257883069
2	5.641737426518615	3.429507328855507
3	3.3774221641628563	3.079608245376038
4	2.8630524711517524	3.4807927737153825
5	2.465708687132593	3.747146213148926
6	2.3405181798388854	4.268272507692817
7	2.0955802307859783	4.458524964431063
8	1.9567820596559982	4.757965787645171
9	1.7989331591552364	4.920921152764451
>10	10.486065752231658	46.688780068160014
>50	0.008164598301763554	0.13979419647288488
>100	0.008164598301763554	0.6774641829070576
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	409	0.3383184991562717	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	298	0.24650100916520531	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027297091618965687	0.0
2	0.0	0.0	0.0	0.11166992025940509	0.0
3	0.0	0.0	0.0	0.155510703768653	0.0
4	0.0	0.0	0.0	0.23243887105846542	0.0
5	0.0	0.0	0.0	0.39787578996128775	0.0
6	0.0	0.0	0.0	0.5633127088641101	0.0
7	0.0	0.0	0.0	0.658438937233233	0.0
8	0.0	0.0	0.0	0.9338914072064322	0.0
9	0.0	0.0	0.0	1.0306720047645832	0.0
10	0.0	0.0	0.0	1.2523574760943652	0.0
11	0.0	0.0	0.0	1.458326440128379	0.0
12	0.0	0.0	0.0	1.6700856963239916	0.0
13	0.0	0.0	0.0	1.7445323098302616	0.0
14	0.0	0.0	0.0	1.7726565860437415	0.0
15	0.0	0.0	0.0	1.8173245541475036	0.0
16	0.0	0.0	0.0	1.9414022433246203	0.0
17	0.0	0.0	0.0	2.0671343016907655	0.0
18	0.0	0.0	0.0	2.2540780200509545	0.0
19	0.0	0.0	0.0	2.330179002746253	0.0
20	0.0	0.0	0.0	2.4253052311153755	0.0
21	0.0	0.0	0.0	2.556000397048605	0.0
22	0.0	0.0	0.0	2.6469907024451578	0.0
23	0.0	0.0	0.0	2.7876120835125566	0.0
24	8.271845945141118E-4	0.0	0.0	2.864540250802369	0.0
25	8.271845945141118E-4	0.0	0.0	2.930715018363498	0.0
26	8.271845945141118E-4	0.0	0.0	3.0241868775435927	0.0
27	8.271845945141118E-4	0.0	0.0	3.0969791218608345	0.0
28	8.271845945141118E-4	0.0	0.0	3.1846606888793305	0.0
29	8.271845945141118E-4	0.0	0.0	3.2599344869801143	0.0
30	8.271845945141118E-4	0.0	0.0	3.360851007510836	0.0
31	8.271845945141118E-4	0.0	0.0	3.513052972901433	0.0
32	8.271845945141118E-4	0.0	0.0	3.6106607550540977	0.0
33	8.271845945141118E-4	0.0	0.0	3.7041326142341924	0.0
34	8.271845945141118E-4	0.0	0.0	3.797604473414287	0.0
35	8.271845945141118E-4	0.0	0.0	3.945670515832313	0.0
36	8.271845945141118E-4	0.0	0.0	4.054031697713662	0.0
37	8.271845945141118E-4	0.0	0.0	4.184726863646891	0.0
38	8.271845945141118E-4	0.0	0.0	4.294742414717268	0.0
39	8.271845945141118E-4	0.0	0.0	4.4055851503821595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAA	20	7.0082274E-4	45.000004	29
GTTGATC	20	7.0082274E-4	45.000004	16
TTTAGAG	30	2.149358E-6	45.000004	1
TGTCAAC	20	7.0082274E-4	45.000004	13
CGAACTA	20	7.0082274E-4	45.000004	36
CATATGC	20	7.0082274E-4	45.000004	33
CTCAAAG	20	7.0082274E-4	45.000004	44
GGCACCG	35	1.1998964E-7	45.000004	8
TGAACGG	20	7.0082274E-4	45.000004	2
GCACGGG	30	2.149358E-6	45.000004	3
ATAGAAC	40	6.728442E-9	45.000004	37
GCACCGA	35	1.1998964E-7	45.000004	9
ATCCGGA	20	7.0082274E-4	45.000004	28
AGCGGGT	20	7.0082274E-4	45.000004	4
GTAGCAT	20	7.0082274E-4	45.000004	29
TAGGGTG	20	7.0082274E-4	45.000004	5
TGTTAAG	20	7.0082274E-4	45.000004	1
GAACGGG	40	6.728442E-9	45.000004	3
GAACGAA	20	7.0082274E-4	45.000004	33
ACTAACC	40	6.728442E-9	45.000004	14
>>END_MODULE