FastQCFastQC Report
Thu 26 May 2016
SRR1548046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548046_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences709014
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84451.191090725994127No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12800.18053240133481144No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.1086015226779725No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT7170.10112635293520297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG302.164321E-645.0000041
TTCGCGG302.164321E-645.0000042
TACACGG453.8380676E-1045.0000042
GATACGA207.031456E-445.09
TAGACGT207.031456E-445.023
TTTCGGT207.031456E-445.041
TTAGACG253.8892886E-545.01
TAGCGCG207.031456E-445.01
CGTCTTA207.031456E-445.018
ATATGCG207.031456E-445.01
CGACAGT207.031456E-445.04
AGACGTA207.031456E-445.024
CGTAATT207.031456E-445.027
ATCGGCC207.031456E-445.038
CGCGTAA351.2110468E-745.031
AGGGCGT406.8084773E-945.06
GCGTAAG551.8189894E-1245.01
CGTTTTT48800.044.077871
CTACGGG1400.043.3928573
CGGTTTT4350.041.8965531