##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548046_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	709014
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43523964265868	31.0	31.0	33.0	30.0	34.0
2	31.813493104508513	31.0	31.0	34.0	30.0	34.0
3	31.67567072018324	31.0	31.0	34.0	30.0	34.0
4	35.52826601449337	37.0	35.0	37.0	33.0	37.0
5	35.508800954565075	37.0	35.0	37.0	33.0	37.0
6	35.61522339474256	37.0	35.0	37.0	33.0	37.0
7	36.01263303686528	37.0	35.0	37.0	35.0	37.0
8	36.048384093967115	37.0	35.0	37.0	35.0	37.0
9	37.8645809532675	39.0	38.0	39.0	35.0	39.0
10	37.271027088322654	39.0	37.0	39.0	34.0	39.0
11	36.93905621045565	39.0	37.0	39.0	33.0	39.0
12	36.2189110511217	38.0	35.0	39.0	32.0	39.0
13	35.94352579779807	38.0	35.0	39.0	31.0	39.0
14	36.99371239495976	39.0	35.0	41.0	32.0	41.0
15	37.237828872208446	39.0	35.0	41.0	32.0	41.0
16	37.360002764402395	39.0	35.0	41.0	33.0	41.0
17	37.28865579523112	39.0	35.0	41.0	32.0	41.0
18	37.142837800099855	39.0	35.0	41.0	32.0	41.0
19	36.98602989503733	38.0	35.0	41.0	32.0	41.0
20	36.76045183875072	38.0	35.0	40.0	32.0	41.0
21	36.62308642706632	38.0	35.0	40.0	32.0	41.0
22	36.53484980550454	38.0	35.0	40.0	31.0	41.0
23	36.450964296896814	38.0	35.0	40.0	31.0	41.0
24	36.41222317189788	38.0	35.0	40.0	31.0	41.0
25	36.37587692203539	38.0	35.0	40.0	31.0	41.0
26	36.242728634413425	37.0	35.0	40.0	31.0	41.0
27	36.17058055271123	37.0	35.0	40.0	31.0	41.0
28	36.11815563585486	37.0	35.0	40.0	31.0	41.0
29	36.06225123904464	37.0	35.0	40.0	31.0	41.0
30	35.9638582594984	37.0	35.0	40.0	30.0	41.0
31	35.77238531256082	37.0	35.0	40.0	30.0	41.0
32	35.52047209222949	37.0	34.0	40.0	29.0	41.0
33	35.28688008981487	37.0	34.0	40.0	27.0	41.0
34	35.02467793301684	37.0	34.0	40.0	26.0	41.0
35	34.75138290640241	37.0	34.0	40.0	24.0	41.0
36	34.50807600414096	37.0	34.0	40.0	23.0	41.0
37	34.35295212788464	37.0	33.0	40.0	23.0	41.0
38	34.25496675665079	37.0	33.0	40.0	23.0	41.0
39	34.20350514940466	36.0	33.0	40.0	23.0	41.0
40	34.075004160707685	36.0	33.0	40.0	22.0	41.0
41	34.023173026202585	36.0	33.0	40.0	21.0	41.0
42	33.97332915852155	36.0	33.0	40.0	21.0	41.0
43	33.84666593325379	36.0	33.0	40.0	21.0	41.0
44	33.67332379896589	36.0	33.0	40.0	20.0	41.0
45	33.65850039632504	35.0	33.0	40.0	20.0	41.0
46	33.597721624678776	35.0	33.0	40.0	20.0	41.0
47	33.5111986505203	35.0	33.0	40.0	20.0	41.0
48	33.379009441280424	35.0	33.0	40.0	20.0	41.0
49	33.25411769020076	35.0	33.0	40.0	18.0	41.0
50	33.08148499183373	35.0	32.0	40.0	18.0	41.0
51	32.68392866713492	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	7.0
15	24.0
16	46.0
17	130.0
18	312.0
19	681.0
20	1188.0
21	2153.0
22	3162.0
23	4822.0
24	7381.0
25	11307.0
26	15199.0
27	16671.0
28	16472.0
29	16769.0
30	18563.0
31	21859.0
32	26849.0
33	34579.0
34	60998.0
35	97093.0
36	47877.0
37	59105.0
38	89391.0
39	156347.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.767697111763663	24.592321167141975	29.157957388711647	17.482024332382718
2	33.80765400965284	25.474673278665865	28.42412138547335	12.293551326207945
3	26.516824773558774	24.237039042952606	35.82792441334022	13.418211770148403
4	24.251848341499603	26.71033294123952	35.047121777567156	13.990696939693715
5	21.987012950378976	30.859052148476618	33.59468219245318	13.559252708691224
6	19.478458817456353	39.26029669371832	30.70842042611288	10.552824062712443
7	84.74529416908551	3.370314267419261	10.401487135655996	1.4829044278392247
8	85.66234235149093	2.400516773998821	10.01376559560178	1.9233752789084562
9	81.22815628464318	4.398079586580801	11.690178191121756	2.6835859376542635
10	47.0057008747359	25.03617700073623	17.810367637310407	10.14775448721746
11	37.53649434284795	21.018625866343964	27.02090508791082	14.423974702897263
12	34.89479756394091	19.82612473096441	28.788289088790915	16.490788616303767
13	21.200991799879834	33.77761228974322	29.35795343956537	15.663442470811578
14	15.705331629558797	36.719021063053766	31.125619522322552	16.450027785064893
15	14.973470199460095	25.37904752233383	45.116598543893346	14.530883734312722
16	17.593164591954462	21.296758597150408	43.989540403997665	17.120536406897465
17	17.746476092150505	21.83468873675273	30.882605985213267	29.536229185883496
18	20.65093213956283	24.513338241557996	35.34020484785914	19.495524771020037
19	24.75014597737139	26.862798195804316	29.022276005833454	19.36477982099084
20	26.47733331076678	24.876800740182844	29.40562527679284	19.24024067225753
21	21.43173477533589	27.695221815084047	31.852685560510796	19.02035784906927
22	22.500119884797762	23.716597979729595	30.32972550612541	23.453556629347233
23	18.586092799295923	28.992657408739458	29.475722623248622	22.945527168715994
24	19.968576078892657	24.566792757265723	38.033663651211405	17.430967512630215
25	17.571867410234496	26.324021810570734	35.534559261171154	20.569551518023623
26	17.534773643397735	32.33278891531056	30.35595912069437	19.776478320597338
27	18.464656551210552	30.79135249797606	31.974403890473248	18.769587060340136
28	16.507008324236192	27.7187756518207	38.662847278050926	17.111368745892182
29	16.640997215851876	24.434919479728187	38.195155525842935	20.72892777857701
30	18.176368872829027	28.10156075902592	34.058847921197604	19.66322244694745
31	25.038856778568547	26.2441926393555	28.581382031948593	20.13556855012736
32	25.316143263743733	26.77859675549425	29.855263788867358	18.04999619189466
33	23.276550251475996	27.40495956356292	29.020019350816767	20.298470834144318
34	19.19412028535403	27.356300439765647	31.22364297460981	22.225936300270515
35	18.85985326100754	26.89171158820559	32.138716583875635	22.109718566911233
36	26.349268138569904	25.18694976403851	29.68883548138683	18.774946616004762
37	20.009336910131534	29.712671400000563	31.4722981492608	18.805693540607095
38	20.075767192185204	30.69064926785649	27.773076413159686	21.460507126798625
39	19.701867664108182	29.77487045389795	29.922258234675198	20.60100364731867
40	21.764591390296946	27.55756585906625	28.247397089479193	22.43044566115761
41	17.618551960892166	25.517549723982885	30.587548341781684	26.27634997334326
42	21.701405049829763	26.99551771897311	27.558271063758966	23.74480616743816
43	21.54964499995769	27.987881762560402	28.148950514376303	22.31352272310561
44	19.679865277695505	30.52619553351556	28.361217126883247	21.432722061905686
45	17.59669061541803	34.01315065710973	26.26619502576818	22.123963701704056
46	21.610010521654015	30.903056921301975	27.406369972948347	20.08056258409566
47	20.78336958085454	28.04274668765356	29.227490571413256	21.946393160078646
48	22.147376497502165	25.367341124434777	30.234381831670458	22.250900546392597
49	20.238105312447992	24.711218678333573	31.666934644449896	23.383741364768536
50	19.069158013805087	30.055259839721078	29.536934390576207	21.33864775589763
51	18.283418945183026	31.746481733788052	27.063781533227832	22.906317787801086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	462.0
1	691.5
2	921.0
3	7086.0
4	13251.0
5	9835.5
6	6420.0
7	6090.5
8	5761.0
9	6015.0
10	6269.0
11	6421.0
12	6573.0
13	6391.0
14	6209.0
15	5851.0
16	5493.0
17	5266.0
18	5039.0
19	4744.0
20	4449.0
21	4328.0
22	4207.0
23	4279.0
24	4351.0
25	4674.5
26	5699.0
27	6400.0
28	7632.0
29	8864.0
30	10021.0
31	11178.0
32	12976.0
33	14774.0
34	16062.5
35	17351.0
36	20093.5
37	22836.0
38	25968.0
39	29100.0
40	36339.5
41	43579.0
42	51578.5
43	59578.0
44	61193.5
45	62809.0
46	62449.5
47	62090.0
48	60967.0
49	59844.0
50	57704.5
51	55565.0
52	49668.0
53	43771.0
54	38331.0
55	32891.0
56	30700.5
57	28510.0
58	25085.0
59	21660.0
60	19531.0
61	17402.0
62	14704.0
63	12006.0
64	10391.0
65	8776.0
66	7242.5
67	5709.0
68	4769.0
69	3829.0
70	3109.5
71	2390.0
72	1987.5
73	1585.0
74	1226.5
75	708.0
76	548.0
77	428.0
78	308.0
79	238.0
80	168.0
81	144.5
82	121.0
83	81.5
84	42.0
85	35.5
86	29.0
87	20.0
88	11.0
89	11.0
90	11.0
91	8.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	709014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.57127290393143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86567052368183	26.476209479020635
2	6.945420027846735	4.663331823745476
3	2.7506092442503665	2.770243607724169
4	1.6166516479335937	2.1709221465347666
5	1.1330293047869333	1.901861799957692
6	0.8371015007969878	1.686153775892775
7	0.6149025968919196	1.445014402271634
8	0.533830149810901	1.4337086114918687
9	0.4385332281985489	1.3249906813165973
>10	5.852738618620316	45.36047323091276
>50	0.3912955199890422	8.17475911264336
>100	0.017690432543736975	0.8580301369806861
>500	0.0016848030994035213	0.3591622524606036
>1k	4.2120077485088034E-4	0.18099515084628845
>5k	4.2120077485088034E-4	1.1941437882007075
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8445	1.191090725994127	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1280	0.18053240133481144	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.1086015226779725	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT	717	0.10112635293520297	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4104093854282144E-4	0.0	0.0	0.031593170233592004	0.0
2	1.4104093854282144E-4	0.0	0.0	0.13314264598442344	0.0
3	1.4104093854282144E-4	0.0	0.0	0.2672725785386466	0.0
4	1.4104093854282144E-4	0.0	0.0	0.3796822065572753	0.0
5	1.4104093854282144E-4	0.0	0.0	0.7301689388361866	0.0
6	1.4104093854282144E-4	0.0	0.0	1.3035003540127557	0.0
7	1.4104093854282144E-4	0.0	0.0	1.6724634492407766	0.0
8	1.4104093854282144E-4	0.0	0.0	2.417723768501045	0.0
9	1.4104093854282144E-4	0.0	0.0	2.8335124553252826	0.0
10	1.4104093854282144E-4	0.0	0.0	3.3673524077098618	0.0
11	1.4104093854282144E-4	0.0	0.0	3.7796150710705287	0.0
12	1.4104093854282144E-4	0.0	0.0	4.117831241696215	0.0
13	1.4104093854282144E-4	0.0	0.0	4.298786765846654	0.0
14	1.4104093854282144E-4	0.0	0.0	4.372974299520179	0.0
15	1.4104093854282144E-4	0.0	0.0	4.4577399035844145	0.0
16	1.4104093854282144E-4	0.0	0.0	4.634746281455655	0.0
17	1.4104093854282144E-4	0.0	0.0	4.860129701247084	0.0
18	1.4104093854282144E-4	0.0	0.0	5.151661321215096	0.0
19	1.4104093854282144E-4	0.0	0.0	5.305113862349685	0.0
20	1.4104093854282144E-4	0.0	0.0	5.470413842321872	0.0
21	1.4104093854282144E-4	0.0	0.0	5.675487366963135	0.0
22	1.4104093854282144E-4	0.0	0.0	5.88916438885551	0.0
23	1.4104093854282144E-4	0.0	0.0	6.168848569985924	0.0
24	1.4104093854282144E-4	0.0	0.0	6.36010008264999	0.0
25	1.4104093854282144E-4	0.0	0.0	6.533298355180575	0.0
26	1.4104093854282144E-4	0.0	0.0	6.698175212337133	0.0
27	1.4104093854282144E-4	0.0	0.0	6.857551472890521	0.0
28	1.4104093854282144E-4	0.0	0.0	7.039212201733675	0.0
29	1.4104093854282144E-4	0.0	0.0	7.229194345950856	0.0
30	1.4104093854282144E-4	0.0	0.0	7.456411297943341	0.0
31	1.4104093854282144E-4	0.0	0.0	7.685461782136883	0.0
32	1.4104093854282144E-4	0.0	0.0	7.886304078621861	0.0
33	1.4104093854282144E-4	0.0	0.0	8.102378796469463	0.0
34	1.4104093854282144E-4	0.0	0.0	8.317184145870181	0.0
35	1.4104093854282144E-4	0.0	0.0	8.545952548186637	0.0
36	1.4104093854282144E-4	0.0	0.0	8.768938272022837	0.0
37	1.4104093854282144E-4	0.0	0.0	8.994180650875723	0.0
38	1.4104093854282144E-4	0.0	0.0	9.23592481953812	0.0
39	1.4104093854282144E-4	0.0	0.0	9.473860882859858	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGCG	30	2.164321E-6	45.000004	1
TTCGCGG	30	2.164321E-6	45.000004	2
TACACGG	45	3.8380676E-10	45.000004	2
GATACGA	20	7.031456E-4	45.0	9
TAGACGT	20	7.031456E-4	45.0	23
TTTCGGT	20	7.031456E-4	45.0	41
TTAGACG	25	3.8892886E-5	45.0	1
TAGCGCG	20	7.031456E-4	45.0	1
CGTCTTA	20	7.031456E-4	45.0	18
ATATGCG	20	7.031456E-4	45.0	1
CGACAGT	20	7.031456E-4	45.0	4
AGACGTA	20	7.031456E-4	45.0	24
CGTAATT	20	7.031456E-4	45.0	27
ATCGGCC	20	7.031456E-4	45.0	38
CGCGTAA	35	1.2110468E-7	45.0	31
AGGGCGT	40	6.8084773E-9	45.0	6
GCGTAAG	55	1.8189894E-12	45.0	1
CGTTTTT	4880	0.0	44.07787	1
CTACGGG	140	0.0	43.392857	3
CGGTTTT	435	0.0	41.896553	1
>>END_MODULE