FastQCFastQC Report
Thu 26 May 2016
SRR1548045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548045_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2385317
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238450.9996574878726812No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41870.17553222485732503No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.0348155E-445.09
TCGGACG207.0348155E-445.030
GCGATCG453.8562575E-1045.09
TCGCGCC253.892072E-545.034
TAGCCGA302.1664873E-644.99999634
CGTTTTT118800.043.2196961
GCGCGAC2600.041.5384649
TGTTGCG1750.041.1428571
TACGGGA8950.040.9776544
ACGCGAG501.0822987E-940.51
CGGGAGT4500.040.56
GCGTAAG2300.040.1086921
CGCAATC451.9297659E-840.020
TCTACGG1300.039.8076932
CTATGCG1250.039.6000021
TTCGTAG1650.039.5454561
CGACGGT2450.038.5714328
ACTATCG356.251117E-638.57142637
CGTTAGG2750.038.4545442
TAGGGAC22900.038.4170345
Produced by FastQC (version 0.11.5)