##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548045_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2385317
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.487731819292783	31.0	31.0	33.0	30.0	34.0
2	31.881308019018018	31.0	31.0	34.0	30.0	34.0
3	31.82670437514175	33.0	31.0	34.0	30.0	34.0
4	35.61337507761023	37.0	35.0	37.0	33.0	37.0
5	35.55817235193477	37.0	35.0	37.0	33.0	37.0
6	35.63568741597029	37.0	35.0	37.0	33.0	37.0
7	36.05704566730544	37.0	35.0	37.0	35.0	37.0
8	36.095791460841475	37.0	35.0	37.0	35.0	37.0
9	37.90675872431211	39.0	38.0	39.0	35.0	39.0
10	37.33792070404059	39.0	37.0	39.0	34.0	39.0
11	36.94351945674307	39.0	37.0	39.0	33.0	39.0
12	36.37274291006185	38.0	35.0	39.0	32.0	39.0
13	36.13215434258843	38.0	35.0	39.0	32.0	39.0
14	37.15365295262642	39.0	35.0	41.0	32.0	41.0
15	37.37894460149322	39.0	35.0	41.0	32.0	41.0
16	37.48693863331373	39.0	35.0	41.0	33.0	41.0
17	37.430398139953724	39.0	35.0	41.0	32.0	41.0
18	37.31077588429546	39.0	35.0	41.0	32.0	41.0
19	37.21731073899193	39.0	35.0	41.0	32.0	41.0
20	37.05829497714559	39.0	35.0	41.0	32.0	41.0
21	36.94580720298392	38.0	35.0	41.0	32.0	41.0
22	36.868400719904315	38.0	35.0	41.0	32.0	41.0
23	36.7958216874319	38.0	35.0	41.0	32.0	41.0
24	36.77191291555798	38.0	35.0	41.0	32.0	41.0
25	36.73932982492474	38.0	35.0	41.0	32.0	41.0
26	36.61081147704896	38.0	35.0	41.0	31.0	41.0
27	36.54355039602703	38.0	35.0	40.0	31.0	41.0
28	36.499923490253074	38.0	35.0	40.0	31.0	41.0
29	36.442103502385635	38.0	35.0	40.0	31.0	41.0
30	36.3523225634161	38.0	35.0	40.0	31.0	41.0
31	36.204255031930764	38.0	35.0	40.0	30.0	41.0
32	36.020854670469376	38.0	35.0	40.0	30.0	41.0
33	35.857025292655024	38.0	35.0	40.0	30.0	41.0
34	35.67120428857045	38.0	35.0	40.0	29.0	41.0
35	35.484602256220036	38.0	35.0	40.0	27.0	41.0
36	35.308744288494985	38.0	34.0	40.0	26.0	41.0
37	35.20836685438455	38.0	34.0	40.0	26.0	41.0
38	35.10515415770734	38.0	34.0	40.0	25.0	41.0
39	35.07921714388486	38.0	34.0	40.0	25.0	41.0
40	34.95549522348602	38.0	34.0	40.0	24.0	41.0
41	34.90875133158402	38.0	34.0	40.0	24.0	41.0
42	34.864888817712696	38.0	34.0	40.0	24.0	41.0
43	34.75560942214389	37.0	34.0	40.0	24.0	41.0
44	34.584222558259555	37.0	34.0	40.0	23.0	41.0
45	34.56896630510745	37.0	34.0	40.0	23.0	41.0
46	34.49985389782574	37.0	34.0	40.0	23.0	41.0
47	34.427015361061024	37.0	33.0	40.0	23.0	41.0
48	34.28838682657273	37.0	33.0	40.0	23.0	41.0
49	34.217252465814816	36.0	33.0	40.0	23.0	41.0
50	34.05382219637893	36.0	33.0	40.0	23.0	41.0
51	33.6905861988155	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	19.0
14	30.0
15	58.0
16	148.0
17	394.0
18	964.0
19	1890.0
20	3393.0
21	5612.0
22	9034.0
23	13275.0
24	19607.0
25	29935.0
26	39797.0
27	45268.0
28	47582.0
29	51414.0
30	58267.0
31	69866.0
32	85635.0
33	110036.0
34	186797.0
35	291647.0
36	160785.0
37	203468.0
38	321798.0
39	628493.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.12152388969684	23.960840424983346	29.586507789111465	15.331127896208343
2	32.10030364936819	25.24528186400382	30.00217581143303	12.652238675194955
3	27.86040597539027	24.646828912048168	33.82963354556229	13.66313156699927
4	25.04832691000819	27.493662267950132	33.040472188811805	14.417538633229881
5	22.58085612939496	31.956046093663858	31.4166209354983	14.046476841442878
6	20.543181472315837	40.62596292232856	28.36583145971793	10.465024145637667
7	87.51415430318067	3.1243226791239906	7.852625038936124	1.5088979787592172
8	88.55376455204905	2.147722923200564	7.624814647277489	1.6736978774728895
9	84.19912321926184	4.677617272672772	8.963211179059218	2.1600483290061656
10	44.81718782031906	33.571764256071624	13.181644200749837	8.429403722859478
11	33.02034907729245	22.773115690702745	29.134073165118096	15.072462066886708
12	32.10617289022801	21.58799019166006	29.736466893079623	16.569370025032313
13	22.085282585081984	32.143568339134795	29.624574008402238	16.14657506738098
14	16.25301794268854	35.49662371919539	31.45607061870603	16.794287719410043
15	15.410236878368789	26.76952371529654	43.029291284973866	14.790948121360808
16	19.018017311745147	23.829369429723595	41.153691521923506	15.998921736607755
17	19.384006402503314	23.02356458282065	31.089997681649862	26.502431333026177
18	21.216341475787075	25.075325417963313	34.89343345140289	18.814899654846716
19	24.284109826911894	26.816184180132037	29.435207144375358	19.464498848580714
20	25.627956368063447	27.249208386139035	28.930200891537687	18.19263435425983
21	22.193108924306497	26.850351546565925	32.52125398846359	18.435285540663987
22	21.813285194378775	23.82869865933962	31.770536159344857	22.587479986936746
23	18.45503134384235	28.858638076197003	30.53652826856975	22.1498023113909
24	18.85061817779356	26.588960712559377	36.488651193950325	18.071769915696738
25	18.25027868413297	27.476138391668698	34.04105198596245	20.232530938235882
26	17.724227010497977	32.22808540751607	29.8887317702427	20.15895581174326
27	18.047664105022516	31.147055087437014	32.30526592482257	18.500014882717895
28	16.275111442210825	28.47596357213737	37.16181119742156	18.087113788230244
29	16.70717141579086	25.909847621930332	36.70233348439641	20.680647477882395
30	19.19048914672557	27.57004624542566	34.02176733742308	19.217697270425692
31	24.57752994675341	26.377164963818228	29.38540244336497	19.659902646063397
32	24.28461290469988	27.715016494662976	30.044392422474665	17.955978178162482
33	23.431351053130463	27.489511876199263	29.919754900501694	19.159382170168577
34	18.7070733156222	28.680590462399756	31.613492043195933	20.998844178782107
35	18.69131021159871	27.89641796038011	32.29424013663593	21.11803169138525
36	24.227345883167732	26.22854740061803	30.262770105608606	19.281336610605635
37	19.614416029399866	29.08816731696458	32.31205747496035	18.985359178675203
38	19.191998380089522	30.530617104560946	28.6514957969947	21.625888718354837
39	18.908346353964696	29.356978548343886	30.50328321141383	21.23139188627759
40	21.225606491715777	27.269038035615388	29.59870742547007	21.906648047198757
41	18.03026599818808	26.330965653621718	30.473769314518783	25.164999033671414
42	20.960861805789335	27.558978534090016	28.573476816708222	22.906682843412426
43	20.970755668953014	27.806953960417	29.24252835157759	21.979762019052394
44	19.92200617360292	30.316347890028872	29.864709805866475	19.896936130501732
45	18.487060629677313	32.31759133062817	27.23566720901247	21.959680830682043
46	21.28522120959185	30.32527752076558	28.88119273035827	19.508308539284297
47	20.95826257055142	28.323405232931304	29.28805689139012	21.430275305127157
48	21.209508002500296	27.056110361851278	30.66787349438251	21.06650814126592
49	20.48008713307288	25.479883805800235	31.615797816390863	22.424231244736028
50	18.901219418634923	30.22700965951276	29.900428328813316	20.971342593038997
51	18.53543994362175	31.23442292995019	27.911761832913612	22.318375293514446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1568.0
1	2251.5
2	2935.0
3	19536.0
4	36137.0
5	25835.5
6	15534.0
7	14599.0
8	13664.0
9	14263.0
10	14862.0
11	15220.5
12	15579.0
13	15419.0
14	15259.0
15	14480.0
16	13701.0
17	13183.0
18	12665.0
19	12593.0
20	12521.0
21	12713.5
22	12906.0
23	13422.5
24	13939.0
25	16294.0
26	22757.5
27	26866.0
28	32674.5
29	38483.0
30	45087.0
31	51691.0
32	59249.0
33	66807.0
34	76121.0
35	85435.0
36	88761.5
37	92088.0
38	101264.0
39	110440.0
40	134030.0
41	157620.0
42	179230.0
43	200840.0
44	213217.5
45	225595.0
46	222149.0
47	218703.0
48	211420.0
49	204137.0
50	193323.5
51	182510.0
52	161368.5
53	140227.0
54	126327.5
55	112428.0
56	98274.5
57	84121.0
58	73732.0
59	63343.0
60	55017.0
61	46691.0
62	39850.0
63	33009.0
64	26113.0
65	19217.0
66	15038.0
67	10859.0
68	8577.0
69	6295.0
70	4719.0
71	3143.0
72	2753.0
73	2363.0
74	1656.0
75	829.0
76	709.0
77	539.5
78	370.0
79	284.5
80	199.0
81	163.5
82	128.0
83	93.0
84	58.0
85	51.0
86	44.0
87	27.5
88	11.0
89	9.0
90	7.0
91	5.5
92	4.0
93	4.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2385317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.20425731665033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48224355676957	18.44326431595954
2	7.576778812048852	3.5162705037225157
3	2.594803072270435	1.80631434524994
4	1.3511642353653135	1.2541105037788731
5	0.8596818322380375	0.9974139222850423
6	0.5995781084294859	0.8347658825656958
7	0.4670054746386304	0.758556064125944
8	0.3601621161952562	0.6685835535923232
9	0.3041527347860078	0.6351874489383685
>10	4.102240602806568	23.2432173177103
>50	1.7136628083410002	28.368289093975896
>100	0.585615860215592	17.744682565580103
>500	0.0016373170661268812	0.24252023756412477
>1k	0.0010915447107512541	0.48022806310347843
>5k	0.0	0.0
>10k+	1.8192411845854237E-4	1.0065961818479643
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23845	0.9996574878726812	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4187	0.17553222485732503	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.384629799728925E-5	0.0	0.0	0.043432382362595834	0.0
2	8.384629799728925E-5	0.0	0.0	0.1560379605729553	0.0
3	8.384629799728925E-5	0.0	0.0	0.27120085087223206	0.0
4	8.384629799728925E-5	0.0	0.0	0.40736723881982984	0.0
5	8.384629799728925E-5	0.0	0.0	0.746735129963858	0.0
6	8.384629799728925E-5	0.0	0.0	1.2253717220813838	0.0
7	1.257694469959339E-4	0.0	0.0	1.5576965241936396	0.0
8	1.257694469959339E-4	0.0	0.0	2.239660388954592	0.0
9	1.257694469959339E-4	0.0	0.0	2.579154049545616	0.0
10	1.257694469959339E-4	0.0	0.0	3.032972137455944	0.0
11	1.257694469959339E-4	0.0	0.0	3.4267981991492116	0.0
12	1.257694469959339E-4	0.0	0.0	3.7445756685589378	0.0
13	1.257694469959339E-4	0.0	0.0	3.894409003080094	0.0
14	1.257694469959339E-4	0.0	0.0	3.9658879721227827	0.0
15	1.257694469959339E-4	0.0	0.0	4.047428496925146	0.0
16	1.257694469959339E-4	0.0	0.0	4.214618015131742	0.0
17	1.257694469959339E-4	0.0	0.0	4.427922997236846	0.0
18	1.257694469959339E-4	0.0	0.0	4.7113654076166815	0.0
19	1.257694469959339E-4	0.0	0.0	4.865432980186701	0.0
20	1.257694469959339E-4	0.0	0.0	5.020297092587694	0.0
21	1.257694469959339E-4	0.0	0.0	5.209035109379592	0.0
22	1.257694469959339E-4	0.0	0.0	5.424436248934628	0.0
23	1.257694469959339E-4	0.0	0.0	5.654762029533182	0.0
24	1.257694469959339E-4	0.0	0.0	5.829288098814539	0.0
25	1.257694469959339E-4	0.0	0.0	5.9781572009087265	0.0
26	1.257694469959339E-4	0.0	0.0	6.128661305813861	0.0
27	1.257694469959339E-4	0.0	0.0	6.284154265449834	0.0
28	1.257694469959339E-4	0.0	0.0	6.4398568408307995	0.0
29	1.257694469959339E-4	0.0	0.0	6.619665226885986	0.0
30	1.257694469959339E-4	0.0	0.0	6.859717178052225	0.0
31	1.257694469959339E-4	0.0	0.0	7.056378670004867	0.0
32	1.257694469959339E-4	0.0	0.0	7.2369835958910285	0.0
33	1.257694469959339E-4	0.0	0.0	7.422367760763035	0.0
34	1.257694469959339E-4	0.0	0.0	7.620245024036637	0.0
35	1.257694469959339E-4	0.0	0.0	7.849355033314231	0.0
36	1.257694469959339E-4	0.0	0.0	8.048489991057792	0.0
37	1.257694469959339E-4	0.0	0.0	8.26070497128893	0.0
38	1.257694469959339E-4	0.0	0.0	8.486083820305645	0.0
39	1.257694469959339E-4	0.0	0.0	8.799165897027523	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.0348155E-4	45.0	9
TCGGACG	20	7.0348155E-4	45.0	30
GCGATCG	45	3.8562575E-10	45.0	9
TCGCGCC	25	3.892072E-5	45.0	34
TAGCCGA	30	2.1664873E-6	44.999996	34
CGTTTTT	11880	0.0	43.219696	1
GCGCGAC	260	0.0	41.538464	9
TGTTGCG	175	0.0	41.142857	1
TACGGGA	895	0.0	40.977654	4
ACGCGAG	50	1.0822987E-9	40.5	1
CGGGAGT	450	0.0	40.5	6
GCGTAAG	230	0.0	40.108692	1
CGCAATC	45	1.9297659E-8	40.0	20
TCTACGG	130	0.0	39.807693	2
CTATGCG	125	0.0	39.600002	1
TTCGTAG	165	0.0	39.545456	1
CGACGGT	245	0.0	38.57143	28
ACTATCG	35	6.251117E-6	38.571426	37
CGTTAGG	275	0.0	38.454544	2
TAGGGAC	2290	0.0	38.417034	5
>>END_MODULE