FastQCFastQC Report
Thu 26 May 2016
SRR1548044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548044_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1616678
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231511.4320105797196474No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27870.17239054406628904No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.12599911670722308No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT17090.10571059914219157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG502.1827873E-1145.01
TGCGAAC207.0341385E-445.021
TCGTAAG551.8189894E-1245.01
CTTTACG207.0341385E-445.03
TAACGCG302.1660526E-644.9999961
CGTTTTT115750.043.386611
CCAATCG1300.043.2692324
CGACGGT3950.041.5822828
CGGTCTA3950.040.4430431
CGGTTTT9050.039.7790031
TACGGGA6450.039.4186024
GGCGATA4650.039.193558
ACTAACC4500.039.014
CGTTAGG2150.038.7209322
GCGCGAC4650.038.709689
ACGGGAT6700.038.61945
GTCGAAC356.249864E-638.5714333
GCGATAA1400.038.571439
CTCACGA4550.038.57142624
TAGGGTA8700.038.5344855
Produced by FastQC (version 0.11.5)