FastQCFastQC Report
Thu 26 May 2016
SRR1548043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548043_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3331770
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310280.9312767688045693No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55620.1669382940599141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGT207.035219E-445.038
CGTTTTT153700.043.2140581
CGACGGT8400.042.85714328
ACGGTCT9200.041.0869630
TCGATTG1700.041.029411
CGGTCTA9000.041.031
ACGGGTA4150.040.1204835
CGTTCGA1150.039.13043614
TAGGGTA18300.039.0983625
CGTAAGG4550.039.0659332
TAGGGAC28600.039.020985
TATAGCG2200.038.8636361
AGTACGG5150.038.44662
TAAGGGA35350.038.1895334
GACGGTC9550.038.1675429
GTAGGGC15900.038.0660364
TAGTACG1900.037.8947371
CACGACG9550.037.69633526
AGGGCGA23500.037.627666
TACGGGA11250.037.64
Produced by FastQC (version 0.11.5)