##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548043_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3331770
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44065556746114	31.0	31.0	33.0	30.0	34.0
2	31.82291574748557	31.0	31.0	34.0	30.0	34.0
3	31.787436707815967	31.0	31.0	34.0	30.0	34.0
4	35.58513672912596	37.0	35.0	37.0	33.0	37.0
5	35.522178601764224	37.0	35.0	37.0	33.0	37.0
6	35.60842945341365	37.0	35.0	37.0	33.0	37.0
7	36.00439195982916	37.0	35.0	37.0	35.0	37.0
8	36.032848305855445	37.0	35.0	37.0	35.0	37.0
9	37.88053677174595	39.0	38.0	39.0	35.0	39.0
10	37.238605906169994	39.0	37.0	39.0	34.0	39.0
11	36.92641328783199	39.0	37.0	39.0	33.0	39.0
12	36.28406912842123	38.0	35.0	39.0	32.0	39.0
13	35.999881144256655	38.0	35.0	39.0	31.0	39.0
14	36.97422751270346	39.0	35.0	41.0	31.0	41.0
15	37.19338189610927	39.0	35.0	41.0	32.0	41.0
16	37.333364547973	39.0	35.0	41.0	32.0	41.0
17	37.27296301965622	39.0	35.0	41.0	32.0	41.0
18	37.16240676877455	39.0	35.0	41.0	32.0	41.0
19	37.061046530822956	38.0	35.0	41.0	32.0	41.0
20	36.89404400663911	38.0	35.0	41.0	32.0	41.0
21	36.77419479735996	38.0	35.0	41.0	32.0	41.0
22	36.70388262094922	38.0	35.0	41.0	31.0	41.0
23	36.62676805421743	38.0	35.0	40.0	31.0	41.0
24	36.6210314637565	38.0	35.0	40.0	31.0	41.0
25	36.59580883434331	38.0	35.0	40.0	31.0	41.0
26	36.46761991373955	38.0	35.0	40.0	31.0	41.0
27	36.40585634662657	38.0	35.0	40.0	31.0	41.0
28	36.34507153855158	38.0	35.0	40.0	31.0	41.0
29	36.26387805880958	38.0	35.0	40.0	31.0	41.0
30	36.16445072739115	38.0	35.0	40.0	30.0	41.0
31	36.04298015769396	38.0	35.0	40.0	30.0	41.0
32	35.86300584974353	38.0	35.0	40.0	30.0	41.0
33	35.69067192513289	38.0	35.0	40.0	29.0	41.0
34	35.48918712876339	38.0	34.0	40.0	28.0	41.0
35	35.29073675553835	38.0	34.0	40.0	27.0	41.0
36	35.10622551976877	38.0	34.0	40.0	25.0	41.0
37	34.98551010423889	38.0	34.0	40.0	25.0	41.0
38	34.849861485036485	37.0	34.0	40.0	24.0	41.0
39	34.794877797687114	37.0	34.0	40.0	24.0	41.0
40	34.64940767219826	37.0	34.0	40.0	23.0	41.0
41	34.59335878527029	37.0	34.0	40.0	23.0	41.0
42	34.56351548876423	37.0	34.0	40.0	23.0	41.0
43	34.45272542822584	37.0	33.0	40.0	23.0	41.0
44	34.2895385936004	37.0	33.0	40.0	23.0	41.0
45	34.254341686250854	36.0	33.0	40.0	23.0	41.0
46	34.19106060742488	36.0	33.0	40.0	23.0	41.0
47	34.07699631126999	36.0	33.0	40.0	23.0	41.0
48	33.95201499503267	36.0	33.0	40.0	23.0	41.0
49	33.84173277267038	36.0	33.0	40.0	23.0	41.0
50	33.661233518520184	35.0	33.0	40.0	22.0	41.0
51	33.279402839931926	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	14.0
13	20.0
14	44.0
15	93.0
16	308.0
17	690.0
18	1625.0
19	3081.0
20	5076.0
21	8583.0
22	13405.0
23	19852.0
24	29614.0
25	43382.0
26	57867.0
27	65761.0
28	68489.0
29	75219.0
30	85662.0
31	102517.0
32	126602.0
33	163285.0
34	278024.0
35	418045.0
36	236094.0
37	288742.0
38	447394.0
39	792140.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.136843179451162	23.62975235385396	30.179424149926316	15.053980316768564
2	31.424648159987033	25.54909252439394	30.589056267389402	12.43720304822962
3	27.962044198729203	24.87506640614448	33.77595092098194	13.386938474144374
4	24.827884277726252	27.012849026193287	33.78678600263524	14.372480693445224
5	22.19213811277489	32.23968041011234	31.700177383192717	13.868004093920048
6	19.992316396389906	40.93187705033661	28.394276915873544	10.68152963739994
7	87.16826191483807	3.3132539160866448	7.946947118198435	1.5715370508768614
8	88.388814353932	2.3178970937369625	7.37673969091504	1.916548861416004
9	84.72472589644543	3.796600605684066	8.964874526152766	2.5137989717177356
10	44.39526137758609	25.812015835426816	16.33636175366247	13.45636103332463
11	36.73083676244159	23.3935415709968	24.59254390309055	15.283077763471068
12	34.81014595845451	21.08152723627381	26.695750306893935	17.41257649837774
13	23.447326796267447	30.72622059746021	27.96789694366658	17.858555662605763
14	17.25677342673714	34.55718732085348	30.372594746936315	17.81344450547307
15	16.89279271978558	25.186792605732087	41.56961614997434	16.350798524507994
16	20.1962920609766	22.45743253585932	39.57055859197964	17.775716811184445
17	20.772622359886785	22.343319016618793	29.811901781935727	27.07215684155869
18	22.281520032895425	25.189824027468884	32.63823733330932	19.890418606326367
19	25.653691581351655	26.953301098215064	27.721421346611564	19.671585973821724
20	26.649378558543958	26.917074107756537	26.8510431392323	19.582504194467205
21	22.61665721223254	27.962284311342017	30.449100628194625	18.97195784823082
22	22.017546229181487	23.5587090345372	30.385500799875143	24.038243936406175
23	19.96542378375458	28.086002335095163	29.38489151412012	22.56368236703014
24	20.51777283545983	25.886360703169785	34.55520639179775	19.040660069572628
25	19.582534208543805	26.465872494199775	32.55764953763315	21.393943759623262
26	18.61467028036149	31.08194743334624	28.761319058638502	21.542063227653767
27	18.77890730752723	30.488989335998586	30.34969400648904	20.382409349985142
28	17.03478931618929	28.055387976961192	35.159990035326565	19.74983267152294
29	18.476875654682047	25.986367606407406	34.21901871977958	21.31773801913097
30	20.299030245184994	28.41645731848237	31.873148506649617	19.41136392968302
31	25.376511583932864	26.157627927497995	27.359031385719902	21.106829102849236
32	26.483040546016078	27.47800718536994	27.437158027114716	18.601794241499263
33	25.029999069563623	27.09706852513829	27.337511292796325	20.535421112501766
34	20.56936703313854	27.954630721808527	29.870069062390257	21.605933182662667
35	19.46740021069882	27.906578185168847	30.40215861238921	22.223862991743125
36	26.341584202991204	25.856376640644463	28.153113810377068	19.648925345987266
37	20.95570822715854	30.129780867226728	29.221194740333218	19.693316165281516
38	21.487317551931856	30.0140165737731	26.215014841960883	22.283651032334166
39	20.42989161916939	28.20803957055859	29.00950545805983	22.352563352212186
40	23.00140766019263	26.183139892609635	28.27935301656477	22.536099430632966
41	18.61391992844644	25.745564669830152	29.106631009943662	26.533884391779743
42	21.86615522680137	26.997211692283678	27.20701008773115	23.929622993183802
43	21.754172706999583	27.180357587708638	27.70284263319497	23.362627072096814
44	21.064179099997897	29.08499086071367	28.16860107390366	21.682228965384766
45	19.38393106366886	31.819363281378966	26.02286472355535	22.773840931396823
46	22.747338501757326	29.350735494947127	27.10319139676508	20.798734606530463
47	21.214009370394717	27.650287985065	28.148911839652797	22.98679080488749
48	21.827767222827507	26.183800202294876	29.4296725164102	22.558760058467424
49	21.591676496276754	24.89355507733127	30.447870051053943	23.06689837533803
50	20.362810157964084	28.66890571678117	28.809281553048375	22.159002572206365
51	19.709493752569955	30.6357881846586	26.318713476620538	23.336004586150906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2135.0
1	3086.0
2	4037.0
3	25283.5
4	46530.0
5	33285.0
6	20040.0
7	19065.0
8	18090.0
9	19646.0
10	21202.0
11	21783.5
12	22365.0
13	22231.5
14	22098.0
15	20658.5
16	19219.0
17	18186.5
18	17154.0
19	15931.0
20	14708.0
21	14598.0
22	14488.0
23	14975.5
24	15463.0
25	16874.5
26	21710.5
27	25135.0
28	29824.0
29	34513.0
30	40971.0
31	47429.0
32	55743.5
33	64058.0
34	71288.0
35	78518.0
36	88940.0
37	99362.0
38	114536.0
39	129710.0
40	161803.0
41	193896.0
42	226505.0
43	259114.0
44	272518.5
45	285923.0
46	299170.5
47	312418.0
48	307977.5
49	303537.0
50	290658.5
51	277780.0
52	251602.5
53	225425.0
54	205642.5
55	185860.0
56	168795.5
57	151731.0
58	137879.0
59	124027.0
60	109082.0
61	94137.0
62	79945.0
63	65753.0
64	54793.0
65	43833.0
66	35326.0
67	26819.0
68	22320.0
69	17821.0
70	14517.5
71	11214.0
72	9539.0
73	7864.0
74	6152.0
75	3500.5
76	2561.0
77	1998.0
78	1435.0
79	1064.5
80	694.0
81	573.5
82	453.0
83	322.5
84	192.0
85	171.5
86	151.0
87	117.0
88	83.0
89	54.0
90	25.0
91	22.5
92	20.0
93	17.5
94	15.0
95	10.5
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3331770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.31666304381123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.63085535498875	17.770949668477552
2	8.06591198757289	3.6000847993540384
3	2.943983535816842	1.9709966572605728
4	1.486040442990537	1.3265385534278313
5	0.9211400776372761	1.0278386364390604
6	0.6293969165155299	0.8427623344014521
7	0.4870399181028864	0.7608374018831273
8	0.3693828319086641	0.6594713755099536
9	0.2992641158725682	0.6010718791528966
>10	2.936100039335007	15.307223846004062
>50	1.1868861036022993	19.448289193469787
>100	1.0385609257436026	34.37672253114594
>500	0.003262649947932169	0.5298247204253285
>1k	0.0019032124696270985	0.6673170264816495
>5k	1.3594374783050704E-4	0.1687405574327575
>10k+	1.3594374783050704E-4	0.9413308191340526
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31028	0.9312767688045693	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5562	0.1669382940599141	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04844271963550905	0.0
2	0.0	0.0	0.0	0.16627798437467173	0.0
3	0.0	0.0	0.0	0.2821023059815053	0.0
4	0.0	0.0	0.0	0.4461592486876345	0.0
5	0.0	0.0	0.0	0.8678270108680971	0.0
6	0.0	0.0	0.0	1.4222170197822779	0.0
7	0.0	0.0	0.0	1.8296581096534275	0.0
8	0.0	0.0	0.0	2.678906407104932	0.0
9	0.0	0.0	0.0	3.10018398628957	0.0
10	0.0	0.0	0.0	3.757882446867581	0.0
11	0.0	0.0	0.0	4.245010910116845	0.0
12	0.0	0.0	0.0	4.685827653169336	0.0
13	0.0	0.0	0.0	4.874586180918851	0.0
14	3.0014076601926304E-5	0.0	0.0	4.961566974911233	0.0
15	3.0014076601926304E-5	0.0	0.0	5.059112723867494	0.0
16	3.0014076601926304E-5	0.0	0.0	5.256575333831567	0.0
17	3.0014076601926304E-5	0.0	0.0	5.476488473093881	0.0
18	3.0014076601926304E-5	0.0	0.0	5.785273293174499	0.0
19	3.0014076601926304E-5	0.0	0.0	5.947139208288688	0.0
20	3.0014076601926304E-5	0.0	0.0	6.121430951116073	0.0
21	3.0014076601926304E-5	0.0	0.0	6.354190115164012	0.0
22	3.0014076601926304E-5	0.0	0.0	6.590220813561561	0.0
23	3.0014076601926304E-5	0.0	0.0	6.859747221446859	0.0
24	3.0014076601926304E-5	0.0	0.0	7.056369437266078	0.0
25	3.0014076601926304E-5	0.0	0.0	7.225888941913758	0.0
26	3.0014076601926304E-5	0.0	0.0	7.388415166713188	0.0
27	3.0014076601926304E-5	0.0	0.0	7.560846036791255	0.0
28	3.0014076601926304E-5	0.0	0.0	7.740450271177182	0.0
29	3.0014076601926304E-5	0.0	0.0	7.945956653670572	0.0
30	3.0014076601926304E-5	0.0	0.0	8.196514165143451	0.0
31	3.0014076601926304E-5	0.0	0.0	8.41552688210771	0.0
32	3.0014076601926304E-5	0.0	0.0	8.615030449280713	0.0
33	3.0014076601926304E-5	0.0	0.0	8.815314382445367	0.0
34	3.0014076601926304E-5	0.0	0.0	9.032136071817682	0.0
35	3.0014076601926304E-5	0.0	0.0	9.288786440840754	0.0
36	3.0014076601926304E-5	0.0	0.0	9.510380368392775	0.0
37	3.0014076601926304E-5	0.0	0.0	9.74587081341149	0.0
38	3.0014076601926304E-5	0.0	0.0	10.02073972693193	0.0
39	3.0014076601926304E-5	0.0	0.0	10.428360901262693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGT	20	7.035219E-4	45.0	38
CGTTTTT	15370	0.0	43.214058	1
CGACGGT	840	0.0	42.857143	28
ACGGTCT	920	0.0	41.08696	30
TCGATTG	170	0.0	41.02941	1
CGGTCTA	900	0.0	41.0	31
ACGGGTA	415	0.0	40.120483	5
CGTTCGA	115	0.0	39.130436	14
TAGGGTA	1830	0.0	39.098362	5
CGTAAGG	455	0.0	39.065933	2
TAGGGAC	2860	0.0	39.02098	5
TATAGCG	220	0.0	38.863636	1
AGTACGG	515	0.0	38.4466	2
TAAGGGA	3535	0.0	38.189533	4
GACGGTC	955	0.0	38.16754	29
GTAGGGC	1590	0.0	38.066036	4
TAGTACG	190	0.0	37.894737	1
CACGACG	955	0.0	37.696335	26
AGGGCGA	2350	0.0	37.62766	6
TACGGGA	1125	0.0	37.6	4
>>END_MODULE