FastQCFastQC Report
Thu 26 May 2016
SRR1548042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548042_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3520969
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466011.3235276993350409No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44340.1259312422233766No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36540.1037782496806987No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT36520.10372144713571747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT275350.043.488291
TAGTACG1650.040.909091
TACGGGA9700.040.1288644
AGTACGG3050.039.8360672
CGGTTTT24000.038.343751
TCGACGG1300.038.076922
TCACGAC3550.037.39436725
ACGGGAT11150.037.331845
GGGCGAT40800.037.279417
CGGGTAT2250.037.06
TAAGGGA33750.036.8666654
TAGGGAT36100.036.7105265
AGGGCGA21950.036.6970376
ATAGGGA37750.036.5364274
TTACGGG9750.036.4615363
TACGTAG1300.036.3461531
TAGGGCG8650.036.1560675
TAGGGAC26350.036.1195455
TTAGGGA35350.036.0891084
GTACGGG10550.036.0426563
Produced by FastQC (version 0.11.5)