##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548042_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3520969
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45568989673013	31.0	31.0	33.0	30.0	34.0
2	31.83174688558746	31.0	31.0	34.0	30.0	34.0
3	31.657557905224387	31.0	31.0	34.0	30.0	34.0
4	35.52792654522093	37.0	35.0	37.0	33.0	37.0
5	35.52802168948377	37.0	35.0	37.0	33.0	37.0
6	35.628377585829355	37.0	35.0	37.0	33.0	37.0
7	35.994493277276796	37.0	35.0	37.0	35.0	37.0
8	36.011196633653974	37.0	35.0	37.0	35.0	37.0
9	37.76351140836514	39.0	38.0	39.0	35.0	39.0
10	37.2799220896293	39.0	37.0	39.0	34.0	39.0
11	37.002608656878266	39.0	37.0	39.0	33.0	39.0
12	36.48556235513576	39.0	35.0	39.0	32.0	39.0
13	36.30105150031142	39.0	35.0	39.0	32.0	39.0
14	37.40301973689629	40.0	35.0	41.0	32.0	41.0
15	37.55495830835205	40.0	35.0	41.0	32.0	41.0
16	37.63286441885742	40.0	35.0	41.0	33.0	41.0
17	37.54556629155213	39.0	35.0	41.0	32.0	41.0
18	37.363525779409024	39.0	36.0	41.0	32.0	41.0
19	37.206310251524506	38.0	35.0	41.0	32.0	41.0
20	36.990837181469075	38.0	35.0	41.0	32.0	41.0
21	36.85618902069288	38.0	35.0	41.0	32.0	41.0
22	36.76826634940552	38.0	35.0	41.0	32.0	41.0
23	36.66176612176932	38.0	35.0	40.0	31.0	41.0
24	36.63711921348924	38.0	35.0	40.0	31.0	41.0
25	36.59308048437802	38.0	35.0	40.0	31.0	41.0
26	36.47016062907682	38.0	35.0	40.0	31.0	41.0
27	36.391163909707814	38.0	35.0	40.0	31.0	41.0
28	36.28173636291601	38.0	35.0	40.0	31.0	41.0
29	36.18904483396474	38.0	35.0	40.0	30.0	41.0
30	36.05327084674702	38.0	35.0	40.0	30.0	41.0
31	35.88260106805825	38.0	35.0	40.0	30.0	41.0
32	35.60641601786327	38.0	34.0	40.0	29.0	41.0
33	35.30492259375189	38.0	34.0	40.0	26.0	41.0
34	34.979616406733484	38.0	34.0	40.0	24.0	41.0
35	34.65548177220532	38.0	34.0	40.0	23.0	41.0
36	34.40006430048092	37.0	33.0	40.0	22.0	41.0
37	34.2736581889815	37.0	33.0	40.0	21.0	41.0
38	34.122894862181404	37.0	33.0	40.0	21.0	41.0
39	34.05401098390812	37.0	33.0	40.0	20.0	41.0
40	33.88677009084715	37.0	33.0	40.0	19.0	41.0
41	33.804587032717414	37.0	33.0	40.0	18.0	41.0
42	33.734270026234256	37.0	33.0	40.0	18.0	41.0
43	33.594116846811204	36.0	33.0	40.0	18.0	41.0
44	33.39788649090634	36.0	33.0	40.0	18.0	41.0
45	33.35643710580809	36.0	33.0	40.0	18.0	41.0
46	33.259607795467666	36.0	32.0	40.0	18.0	41.0
47	33.16413663397775	35.0	32.0	40.0	18.0	41.0
48	33.0069674001674	35.0	32.0	40.0	18.0	41.0
49	32.880665805350745	35.0	32.0	40.0	17.0	41.0
50	32.68997341356882	35.0	31.0	40.0	15.0	41.0
51	32.31658557630016	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	7.0
12	23.0
13	50.0
14	71.0
15	177.0
16	378.0
17	897.0
18	2022.0
19	4079.0
20	7121.0
21	11445.0
22	17785.0
23	26161.0
24	39589.0
25	59906.0
26	80613.0
27	88735.0
28	87359.0
29	87780.0
30	93943.0
31	108406.0
32	128747.0
33	162640.0
34	264972.0
35	392262.0
36	251852.0
37	316572.0
38	479841.0
39	807411.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.605163237733702	22.92939812875376	27.317707142550812	18.14773149096172
2	36.80003998899167	23.925601162634493	26.101365845595346	13.172993002778496
3	28.43129263563525	24.096860835752885	33.834492720611856	13.637353808000013
4	24.890221981505658	26.02505162641307	34.184396397696204	14.90032999438507
5	22.37256278030281	30.13783989577869	32.52292195699536	14.966675366923141
6	20.70844702126034	38.593182728958986	30.163088627022844	10.535281622757827
7	81.29557516694979	4.161837266956909	12.113625538878644	2.4289620272146673
8	81.57532770098231	3.5533968063905137	12.25665434714137	2.614621145485802
9	76.21987015506242	5.727400610456951	14.201630289843505	3.8510989446371156
10	40.81345220591263	27.23491743324068	20.293220417447582	11.658409943399104
11	33.33801007620345	22.454472050165737	28.65762237611294	15.549895497517872
12	30.352667120897685	20.69401917483511	31.729163193427716	17.224150510839486
13	22.777536524746456	28.37602375936851	31.454750098623418	17.391689617261612
14	17.956874939824804	31.00987256633046	33.20242240133327	17.830830092511466
15	17.082342957293857	24.437931717092653	42.0839547295077	16.395770596105788
16	20.529490603296992	21.95875624011458	40.11710980698779	17.394643349600635
17	20.017046443748864	22.500340105238077	32.457201412452086	25.025412038560972
18	22.646720263654693	23.744628254324308	34.45903670268043	19.149614779340574
19	25.18641885231026	25.5054787474698	30.302822887676662	19.00527951254328
20	26.773510360358188	24.160735297584274	29.958826675270362	19.106927666787183
21	23.0118754240665	26.341271394323552	31.91615717150591	18.730696010104037
22	22.867170940726826	23.170610136016535	31.452193984099264	22.510024939157375
23	20.723158880410477	26.892114074278982	30.883344897384784	21.501382147925753
24	20.444031174372736	23.947612148814716	36.094353571417415	19.514003105395133
25	19.925083123424262	25.353219525647624	34.041793608520834	20.679903742407273
26	19.510708557786224	28.915193516330305	30.83795966394478	20.736138261938688
27	19.81977120502907	28.002859440114353	32.59017617025313	19.587193184603443
28	18.580396476083713	26.520824239009205	36.336616425762344	18.562162859144742
29	20.017841679378602	24.38766146478427	35.334505927203566	20.259990928633567
30	20.561782850118817	26.08526232409317	33.41526153737792	19.937693288410095
31	24.941969099983556	25.414736681862294	29.36864823291543	20.274645985238724
32	26.006789608201608	25.727690303436358	29.839001706632462	18.426518381729576
33	24.15502664181366	26.597479273461367	29.25794007274702	19.989554011977955
34	21.442165494782827	27.182914703310367	30.608676191128065	20.766243610778737
35	22.132259613759736	26.33581835000535	30.97329172736255	20.55863030887236
36	25.1748027318616	26.391456442814466	28.833028635014962	19.600712190308975
37	22.41329020505435	28.42862291602113	29.504065500150666	19.65402137877385
38	21.734897410343574	29.187646923332757	27.2454543053347	21.83200136098898
39	21.917460789913232	28.18800733548066	28.581876182380476	21.312655692225636
40	23.045360524332935	26.608896585002594	28.719877965412362	21.62586492525211
41	19.702814764912727	26.150471645731617	29.881490010278423	24.265223579077237
42	22.93590202015411	26.46822508235659	27.531029100227805	23.064843797261492
43	22.480004794134796	27.026026074072224	27.98263773410104	22.51133139769194
44	21.64631384144535	28.398403962091116	28.65344738905682	21.30183480740671
45	20.54249838609769	30.203929656864346	27.208703058731842	22.04486889830612
46	22.544447281415998	29.003635078866076	27.47516379723877	20.97675384247916
47	21.98690190115278	27.819273614735035	28.621694766412315	21.572129717699873
48	22.466457387156773	26.005625156029488	29.916395174169384	21.61152228264435
49	21.70981908673436	25.51300508467981	29.751497386088886	23.02567844249694
50	20.07379218618511	28.333535455722558	29.935480829283073	21.657191528809257
51	19.634850519842693	29.303694522729394	28.205786532059783	22.85566842536813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2015.0
1	3616.0
2	5217.0
3	41000.0
4	76783.0
5	56448.0
6	36113.0
7	33725.5
8	31338.0
9	31797.0
10	32256.0
11	32254.5
12	32253.0
13	31185.5
14	30118.0
15	28512.0
16	26906.0
17	25448.0
18	23990.0
19	22645.5
20	21301.0
21	21241.5
22	21182.0
23	21601.5
24	22021.0
25	23917.5
26	30024.0
27	34234.0
28	38319.5
29	42405.0
30	50221.0
31	58037.0
32	65770.5
33	73504.0
34	83278.0
35	93052.0
36	99645.5
37	106239.0
38	116006.0
39	125773.0
40	153519.5
41	181266.0
42	210349.0
43	239432.0
44	247306.5
45	255181.0
46	251736.5
47	248292.0
48	254763.5
49	261235.0
50	260952.0
51	260669.0
52	250270.0
53	239871.0
54	215355.0
55	190839.0
56	174481.0
57	158123.0
58	147482.0
59	136841.0
60	127927.5
61	119014.0
62	108029.0
63	97044.0
64	83631.5
65	70219.0
66	59423.5
67	48628.0
68	40988.5
69	33349.0
70	27671.0
71	21993.0
72	18362.5
73	14732.0
74	12371.0
75	8110.5
76	6211.0
77	4940.0
78	3669.0
79	2759.5
80	1850.0
81	1396.5
82	943.0
83	734.5
84	526.0
85	381.0
86	236.0
87	172.0
88	108.0
89	76.0
90	44.0
91	38.5
92	33.0
93	32.0
94	31.0
95	23.5
96	16.0
97	14.5
98	13.0
99	6.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3520969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.110228568872955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.46064081961121	20.454953103473
2	6.700448473419214	3.364995853630247
3	2.406699260633718	1.8129830559315059
4	1.217736206849076	1.2231053796229063
5	0.7965934301869793	1.0001321554228797
6	0.6122613308056816	0.92244131762478
7	0.4429611227320207	0.7785998527247938
8	0.37974434467892254	0.7628373834099696
9	0.30146453229441555	0.6812858980189042
>10	3.67884307574792	22.744111431059817
>50	1.4841734372519524	26.583454196794136
>100	0.5141979301744769	17.110079434982044
>500	0.0028621862260386367	0.49176431670358994
>1k	0.001259361939456992	0.7295682701568833
>5k	0.0	0.0
>10k+	1.1448744904154474E-4	1.3396883504445158
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46601	1.3235276993350409	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4434	0.1259312422233766	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3654	0.1037782496806987	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	3652	0.10372144713571747	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7040763494367603E-4	0.0	0.0	0.031411807374617615	0.0
2	1.7040763494367603E-4	0.0	0.0	0.12343193024420267	0.0
3	1.7040763494367603E-4	0.0	0.0	0.25089684118207234	0.0
4	1.7040763494367603E-4	0.0	0.0	0.35637916721220775	0.0
5	1.7040763494367603E-4	0.0	0.0	0.6561261970781339	0.0
6	1.7040763494367603E-4	0.0	0.0	1.1892464829994243	0.0
7	1.7040763494367603E-4	0.0	0.0	1.5547424586811187	0.0
8	1.7040763494367603E-4	0.0	0.0	2.2532717555877375	0.0
9	1.9880890743428868E-4	0.0	0.0	2.6698900217525345	0.0
10	2.2721017992490135E-4	0.0	0.0	3.2046575814782807	0.0
11	2.2721017992490135E-4	0.0	0.0	3.5432575521113647	0.0
12	2.55611452415514E-4	0.0	0.0	3.8203971690747633	0.0
13	2.55611452415514E-4	0.0	0.0	3.971406734907351	0.0
14	2.55611452415514E-4	0.0	0.0	4.043659572123469	0.0
15	2.55611452415514E-4	0.0	0.0	4.117616485689025	0.0
16	2.55611452415514E-4	0.0	0.0	4.257691561612726	0.0
17	2.55611452415514E-4	0.0	0.0	4.408473917265389	0.0
18	2.55611452415514E-4	0.0	0.0	4.633099581393645	0.0
19	2.55611452415514E-4	0.0	0.0	4.738468302333818	0.0
20	2.55611452415514E-4	0.0	0.0	4.868233716343427	0.0
21	2.55611452415514E-4	0.0	0.0	5.011461333513587	0.0
22	2.55611452415514E-4	0.0	0.0	5.166134663497464	0.0
23	2.55611452415514E-4	0.0	0.0	5.352020991948523	0.0
24	2.55611452415514E-4	0.0	0.0	5.492039265327244	0.0
25	2.55611452415514E-4	0.0	0.0	5.618936150815301	0.0
26	2.55611452415514E-4	0.0	0.0	5.741970463244635	2.840127249061267E-5
27	2.55611452415514E-4	0.0	0.0	5.868696941097749	2.840127249061267E-5
28	2.55611452415514E-4	0.0	0.0	6.002381730711063	2.840127249061267E-5
29	2.55611452415514E-4	0.0	0.0	6.141065144282725	2.840127249061267E-5
30	2.840127249061267E-4	0.0	0.0	6.3253610014743105	2.840127249061267E-5
31	3.1241399739673936E-4	0.0	0.0	6.483783299426947	2.840127249061267E-5
32	3.1241399739673936E-4	0.0	0.0	6.6451877309911	2.840127249061267E-5
33	3.1241399739673936E-4	0.0	0.0	6.803013602221434	2.840127249061267E-5
34	3.1241399739673936E-4	0.0	0.0	6.960186244184484	2.840127249061267E-5
35	3.1241399739673936E-4	0.0	0.0	7.1353368916340925	2.840127249061267E-5
36	3.1241399739673936E-4	0.0	0.0	7.3052049024004475	2.840127249061267E-5
37	3.1241399739673936E-4	0.0	0.0	7.47640777297386	2.840127249061267E-5
38	3.1241399739673936E-4	0.0	0.0	7.65672745201676	2.840127249061267E-5
39	3.1241399739673936E-4	0.0	0.0	7.872861135670322	2.840127249061267E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27535	0.0	43.48829	1
TAGTACG	165	0.0	40.90909	1
TACGGGA	970	0.0	40.128864	4
AGTACGG	305	0.0	39.836067	2
CGGTTTT	2400	0.0	38.34375	1
TCGACGG	130	0.0	38.07692	2
TCACGAC	355	0.0	37.394367	25
ACGGGAT	1115	0.0	37.33184	5
GGGCGAT	4080	0.0	37.27941	7
CGGGTAT	225	0.0	37.0	6
TAAGGGA	3375	0.0	36.866665	4
TAGGGAT	3610	0.0	36.710526	5
AGGGCGA	2195	0.0	36.697037	6
ATAGGGA	3775	0.0	36.536427	4
TTACGGG	975	0.0	36.461536	3
TACGTAG	130	0.0	36.346153	1
TAGGGCG	865	0.0	36.156067	5
TAGGGAC	2635	0.0	36.119545	5
TTAGGGA	3535	0.0	36.089108	4
GTACGGG	1055	0.0	36.042656	3
>>END_MODULE