FastQCFastQC Report
Thu 26 May 2016
SRR1548041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548041_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1930781
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145440.7532703087507077No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30380.15734565442688736No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC253.891794E-545.00000439
TAGTCGA207.034478E-445.00000428
CGCATCG900.042.521
CGTTTTT77200.042.4935231
CGCGTAA700.041.78571331
CGGTCTA1850.041.35135331
ATACGCG603.6379788E-1241.2500041
TATAGCG1600.040.7812541
GGCGATA5650.040.6194658
TATAACG451.929402E-840.01
ACGGGAC4650.039.677425
ACGGGAT9450.039.523815
CGACAGG3250.039.4615362
GGGCGAT21400.039.427577
CGTAAGG3900.039.230772
GCGTAAC750.039.032
GCGTAAG1850.038.9189221
TAATGCG1050.038.571431
GTTAGCG700.038.5714261
TATTGCG700.038.5714261
Produced by FastQC (version 0.11.5)