FastQCFastQC Report
Thu 26 May 2016
SRR1548040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548040_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences275306
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.32182371615584116No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5450.19796154097622282No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC3290.11950338895628863No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT207.02393E-445.00000420
GAGTCTA207.02393E-445.0000049
AAGACGG253.8830527E-545.0000042
TAACGCG207.02393E-445.0000041
ACGGGCT253.8830527E-545.0000045
CGTTAGG207.02393E-445.0000042
CGGGGTA253.8830527E-545.0000046
AATGCGG207.02393E-445.0000042
CTACGGG406.7830115E-945.0000043
GTATGCG253.8830527E-545.0000041
TATTACG253.8830527E-545.0000041
TAGGGTC406.7830115E-945.0000045
CTCGAGG207.02393E-445.0000042
TGTAACG207.02393E-445.0000041
AAGTCGG207.02393E-445.0000042
GACCGAT1100.045.09
TTACGGG750.045.03
TGGGCGA1100.045.06
AGACGGG1100.042.9545443
AACGGGA1000.042.7500044