##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548040_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	275306
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495782874328928	31.0	31.0	33.0	30.0	34.0
2	31.918461639048914	31.0	31.0	34.0	30.0	34.0
3	32.02705353315947	33.0	31.0	34.0	30.0	34.0
4	35.74555948653498	37.0	35.0	37.0	35.0	37.0
5	35.645532607353275	37.0	35.0	37.0	33.0	37.0
6	35.696668434396635	37.0	35.0	37.0	33.0	37.0
7	36.01391179269613	37.0	35.0	37.0	35.0	37.0
8	36.02261483585538	37.0	35.0	37.0	35.0	37.0
9	37.82020733293135	39.0	38.0	39.0	35.0	39.0
10	37.31104298489681	39.0	37.0	39.0	34.0	39.0
11	36.95471947578331	39.0	37.0	39.0	33.0	39.0
12	36.03249475129492	37.0	35.0	39.0	32.0	39.0
13	35.64058175266794	37.0	35.0	39.0	31.0	39.0
14	36.62125780041118	38.0	35.0	41.0	31.0	41.0
15	36.899152216079564	38.0	35.0	41.0	32.0	41.0
16	37.06305347504232	38.0	35.0	41.0	32.0	41.0
17	36.982495840991476	38.0	35.0	41.0	32.0	41.0
18	36.949663283764245	38.0	35.0	41.0	32.0	41.0
19	36.894375712843164	38.0	35.0	41.0	32.0	41.0
20	36.74837816829274	38.0	35.0	41.0	32.0	41.0
21	36.590528357536705	38.0	35.0	41.0	31.0	41.0
22	36.45815928457789	38.0	35.0	40.0	31.0	41.0
23	36.407270455420516	38.0	35.0	40.0	31.0	41.0
24	36.39366014543817	38.0	35.0	40.0	31.0	41.0
25	36.356461537343904	38.0	35.0	40.0	31.0	41.0
26	36.20799038161174	38.0	35.0	40.0	31.0	41.0
27	36.11858804384939	37.0	35.0	40.0	31.0	41.0
28	36.046875113510055	37.0	35.0	40.0	30.0	41.0
29	36.00357420470313	37.0	35.0	40.0	30.0	41.0
30	35.95926714274298	37.0	35.0	40.0	30.0	41.0
31	35.883772965354915	37.0	35.0	40.0	30.0	41.0
32	35.74749188176066	37.0	34.0	40.0	30.0	41.0
33	35.61904208408099	37.0	34.0	40.0	30.0	41.0
34	35.52198644417484	37.0	34.0	40.0	29.0	41.0
35	35.42527950716657	37.0	34.0	40.0	29.0	41.0
36	35.211502836843366	37.0	34.0	40.0	27.0	41.0
37	35.09943117839786	36.0	34.0	40.0	27.0	41.0
38	34.997206744495216	36.0	34.0	40.0	27.0	41.0
39	34.97340050707213	36.0	34.0	40.0	27.0	41.0
40	34.80283393750953	36.0	34.0	40.0	26.0	41.0
41	34.77701539378001	36.0	34.0	40.0	26.0	41.0
42	34.72731796619035	36.0	34.0	40.0	26.0	41.0
43	34.60379722926489	35.0	33.0	40.0	26.0	41.0
44	34.453273811685904	35.0	34.0	40.0	25.0	41.0
45	34.41658372865103	35.0	34.0	40.0	25.0	41.0
46	34.33128228226047	35.0	33.0	40.0	24.0	41.0
47	34.257818572788096	35.0	33.0	40.0	24.0	41.0
48	34.14688019876065	35.0	33.0	40.0	24.0	41.0
49	34.04097622282115	35.0	33.0	39.0	24.0	41.0
50	33.865814766114795	35.0	33.0	39.0	24.0	41.0
51	33.469797243794176	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	6.0
16	34.0
17	66.0
18	142.0
19	263.0
20	425.0
21	739.0
22	1079.0
23	1546.0
24	2104.0
25	2963.0
26	3884.0
27	4504.0
28	5090.0
29	5869.0
30	7136.0
31	8621.0
32	10800.0
33	14212.0
34	26787.0
35	43107.0
36	18209.0
37	22901.0
38	34528.0
39	60273.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.868175775319102	24.734658888654806	30.236173566867414	13.16099176915868
2	29.386573485503405	26.616564840577396	30.274676178506827	13.72218549541238
3	28.966313847137364	25.891190166578284	31.219079860228256	13.923416126056098
4	27.277647417782397	27.10438566540504	30.323349291334008	15.294617625478557
5	24.25446593971799	33.09481086500113	27.919478689167686	14.731244506113198
6	22.01586598185292	42.37648289539639	24.27081138805547	11.33683973469521
7	88.17243358299493	4.425257713235455	5.467734084981802	1.9345746187878217
8	88.92323450996345	3.5444196639375822	5.231633164551445	2.3007126615475144
9	84.07008928247114	5.582878687714761	7.217060289278113	3.1299717405359853
10	52.28218782009836	24.309313999694883	12.142488721640648	11.266009458566105
11	45.053867333076646	20.215324039432485	19.937814649880497	14.792993977610369
12	40.979128678633955	20.303589460454912	22.93302724967854	15.784254611232592
13	22.738698030555092	36.41584273499306	23.518920764531103	17.326538469920745
14	16.154751440215616	39.44955794643052	26.885356657682724	17.510333955671147
15	15.787886933085366	23.93191575919159	44.45235483425715	15.82784247346589
16	18.181950266249192	19.953070401662153	42.78766172913049	19.077317602958164
17	18.10458181078509	20.314849658198515	27.779271065650583	33.801297465365806
18	22.50441327105112	24.040158950404276	32.039621366770064	21.415806411774536
19	28.363711651762042	25.07646037500091	25.06120462321925	21.498623350017798
20	30.910332502742406	23.909758595889667	24.36452529185706	20.81538360951087
21	23.68782373068513	27.100753343552263	27.879886380972447	21.33153654479016
22	23.866170733656368	23.957342012161014	25.99325841064125	26.183228843541368
23	22.00569548066515	28.638315183831807	24.53233856145525	24.82365077404779
24	21.888371484820528	23.61771991892658	35.2495041880671	19.2444044081858
25	19.21716199428999	25.39065621526592	32.73666393031754	22.65551786012655
26	18.43367017064648	32.49620422366385	26.853755457563583	22.216370148126085
27	19.72205473182568	31.58340174206156	27.760746224201434	20.933797301911326
28	17.115137338089255	28.44398596470836	35.44020108533777	19.000675611864615
29	18.143810886795055	23.94753474315852	34.89716896834795	23.011485401698472
30	19.31668761305602	28.837729653549143	30.872193123288266	20.973389610106572
31	28.10690649677087	26.59368121290491	23.54362055312997	21.75579173719425
32	29.529686966502727	26.12293230078531	25.262435253862975	19.08494547884899
33	26.679040776445117	27.632888494983764	24.430996781762836	21.257073946808276
34	20.29995713860214	27.855186592373578	27.770190261018644	24.07466600800564
35	21.1826113488264	25.893369559689944	29.457766993817785	23.466252097665873
36	29.05675866127146	25.333265529992083	25.919522277029923	19.690453531706538
37	22.10086231320785	29.5769071505888	28.392770226584236	19.929460309619117
38	21.956295903467414	30.586692625660174	23.837475391019446	23.619536079852963
39	21.90907571938134	29.278330294290715	27.098937182625875	21.71365680370206
40	23.701263321540395	26.373925740812044	26.02159052109289	23.90322041655467
41	18.786731854736185	24.602805605399084	27.838841144036092	28.77162139582864
42	23.536719141609698	25.464755581062526	24.09972902878978	26.89879624853799
43	22.82369436191002	25.396104698045086	26.380827152332316	25.39937378771258
44	20.736925457490937	29.050220481936464	26.605304642833794	23.607549417738806
45	19.18519756198557	33.84851764945188	23.323865081037102	23.642419707525445
46	22.94610360834853	30.537293048462438	25.688869839378725	20.827733503810304
47	22.79754164457004	26.48180569983945	27.222799357805492	23.49785329778501
48	24.031804610142895	23.311515186737665	29.272155347141	23.38452485597844
49	21.807007475318375	23.631159509781842	30.385461995016456	24.17637101988333
50	20.25636927636884	29.561288166621868	27.576950738451032	22.60539181855826
51	19.733678161754558	31.80097782104277	24.002019570950143	24.463324446252532
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	150.0
1	334.5
2	519.0
3	1063.0
4	1607.0
5	1236.0
6	865.0
7	827.0
8	789.0
9	807.0
10	825.0
11	831.0
12	837.0
13	826.0
14	815.0
15	759.0
16	703.0
17	725.5
18	748.0
19	678.0
20	608.0
21	680.5
22	753.0
23	790.5
24	828.0
25	985.5
26	1458.0
27	1773.0
28	2380.5
29	2988.0
30	3415.5
31	3843.0
32	4726.5
33	5610.0
34	6243.0
35	6876.0
36	7199.5
37	7523.0
38	8779.5
39	10036.0
40	13958.5
41	17881.0
42	21350.5
43	24820.0
44	25041.5
45	25263.0
46	24168.0
47	23073.0
48	22456.5
49	21840.0
50	21371.5
51	20903.0
52	19591.0
53	18279.0
54	16805.0
55	15331.0
56	14291.0
57	13251.0
58	11986.0
59	10721.0
60	9995.0
61	9269.0
62	8267.0
63	7265.0
64	6469.0
65	5673.0
66	4821.0
67	3969.0
68	3279.5
69	2590.0
70	2234.0
71	1878.0
72	1630.0
73	1382.0
74	1157.5
75	719.0
76	505.0
77	409.5
78	314.0
79	229.5
80	145.0
81	119.5
82	94.0
83	69.0
84	44.0
85	32.0
86	20.0
87	17.5
88	15.0
89	10.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	275306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.69384612031703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.56161984331732	21.965013475914073
2	5.300466261153582	3.253833915715604
3	2.7869162860050647	2.5662353889853473
4	2.1017254029490426	2.580401444211169
5	1.9159309838820384	2.9403645398211444
6	1.6638659440013255	3.0642267150007627
7	1.5230408747721949	3.2723587571647546
8	1.3798489976568602	3.3882298242682687
9	1.3538141109086177	3.739838579616863
>10	10.346500674540247	51.10023028920546
>50	0.05443658138268917	1.0519204085635618
>100	0.009467231544815507	0.5575614044009212
>500	0.0023668078862038766	0.5197852571320639
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.32182371615584116	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	545	0.19796154097622282	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	329	0.11950338895628863	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038139379454134675	0.0
2	0.0	0.0	0.0	0.13693853384960736	0.0
3	0.0	0.0	0.0	0.22447749050147836	0.0
4	0.0	0.0	0.0	0.3603263277952533	0.0
5	0.0	0.0	0.0	0.6156785540453168	0.0
6	0.0	0.0	0.0	0.9117127850464574	0.0
7	0.0	0.0	0.0	1.1085846294668478	0.0
8	0.0	0.0	0.0	1.6726842132027635	0.0
9	0.0	0.0	0.0	1.896435239333687	0.0
10	0.0	0.0	0.0	2.260030656796437	0.0
11	0.0	0.0	0.0	2.5825808373228334	0.0
12	0.0	0.0	0.0	2.832484580793735	0.0
13	0.0	0.0	0.0	2.9450865582297516	0.0
14	0.0	0.0	0.0	2.9894008848336036	0.0
15	0.0	0.0	0.0	3.056598839109936	0.0
16	0.0	0.0	0.0	3.198622623553428	0.0
17	0.0	0.0	0.0	3.3497272126288564	0.0
18	0.0	0.0	0.0	3.5821958112064394	0.0
19	0.0	0.0	0.0	3.6882596093074613	0.0
20	0.0	0.0	0.0	3.816117338525132	0.0
21	0.0	0.0	0.0	3.97448657130611	0.0
22	0.0	0.0	0.0	4.1259543925668165	0.0
23	0.0	0.0	0.0	4.294130894350286	0.0
24	0.0	0.0	0.0	4.428526802902952	0.0
25	0.0	0.0	0.0	4.540039083783136	0.0
26	0.0	0.0	0.0	4.64573964969888	0.0
27	0.0	0.0	0.0	4.7459917328354635	0.0
28	0.0	0.0	0.0	4.863678960865364	0.0
29	0.0	0.0	0.0	4.976644170486659	0.0
30	0.0	0.0	0.0	5.110676846854046	0.0
31	0.0	0.0	0.0	5.247615380703653	0.0
32	0.0	0.0	0.0	5.364576144362999	0.0
33	0.0	0.0	0.0	5.496792659804	0.0
34	0.0	0.0	0.0	5.6253768533922255	0.0
35	0.0	0.0	0.0	5.768127102206273	0.0
36	0.0	0.0	0.0	5.90361270731477	0.0
37	0.0	0.0	0.0	6.045273259572984	0.0
38	0.0	0.0	0.0	6.180758864681482	0.0
39	0.0	0.0	0.0	6.315881237604701	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.02393E-4	45.000004	20
GAGTCTA	20	7.02393E-4	45.000004	9
AAGACGG	25	3.8830527E-5	45.000004	2
TAACGCG	20	7.02393E-4	45.000004	1
ACGGGCT	25	3.8830527E-5	45.000004	5
CGTTAGG	20	7.02393E-4	45.000004	2
CGGGGTA	25	3.8830527E-5	45.000004	6
AATGCGG	20	7.02393E-4	45.000004	2
CTACGGG	40	6.7830115E-9	45.000004	3
GTATGCG	25	3.8830527E-5	45.000004	1
TATTACG	25	3.8830527E-5	45.000004	1
TAGGGTC	40	6.7830115E-9	45.000004	5
CTCGAGG	20	7.02393E-4	45.000004	2
TGTAACG	20	7.02393E-4	45.000004	1
AAGTCGG	20	7.02393E-4	45.000004	2
GACCGAT	110	0.0	45.0	9
TTACGGG	75	0.0	45.0	3
TGGGCGA	110	0.0	45.0	6
AGACGGG	110	0.0	42.954544	3
AACGGGA	100	0.0	42.750004	4
>>END_MODULE