FastQCFastQC Report
Thu 26 May 2016
SRR1548039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548039_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2284197
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153080.6701698671349274No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49030.21464873651440747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA4400.041.42045631
CGTTTTT82500.041.3999981
CACGACG4500.041.026
GCGTATG1100.040.9090921
TAATGCG1000.040.51
TAGGGAC19500.039.807695
TACGGGA7300.039.4520574
TAAGGGA25150.039.2743534
TATAGCG750.039.01
ATAGCGG4800.038.9062462
AACGGGA8150.038.6503074
TAATACG356.250999E-638.5714261
ACGGGTA1400.038.5714265
CGACGGT4800.038.43749628
ACGGGAT11800.038.326275
CTATGCG601.5643309E-1037.4999961
CGTAAGG2400.037.4999962
TTACGGG7450.037.4496653
GCGATAC1750.037.2857139
ACGGTCT4950.037.27272830
Produced by FastQC (version 0.11.5)