##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548039_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2284197
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.482453571211238	31.0	31.0	33.0	30.0	34.0
2	31.881154734026882	31.0	31.0	34.0	30.0	34.0
3	31.9210479656527	33.0	31.0	34.0	30.0	34.0
4	35.68381404931361	37.0	35.0	37.0	33.0	37.0
5	35.61252904193465	37.0	35.0	37.0	33.0	37.0
6	35.68063043599129	37.0	35.0	37.0	33.0	37.0
7	36.01898741658447	37.0	35.0	37.0	35.0	37.0
8	36.035981572517606	37.0	35.0	37.0	35.0	37.0
9	37.82872711942096	39.0	38.0	39.0	35.0	39.0
10	37.3064363537821	39.0	37.0	39.0	34.0	39.0
11	36.978180515953746	39.0	37.0	39.0	33.0	39.0
12	36.32517116518409	38.0	35.0	39.0	32.0	39.0
13	36.0582130175287	38.0	35.0	39.0	32.0	39.0
14	37.07756861601692	39.0	35.0	41.0	32.0	41.0
15	37.277861760609966	39.0	35.0	41.0	32.0	41.0
16	37.41751521431821	39.0	35.0	41.0	33.0	41.0
17	37.344396302070265	39.0	35.0	41.0	32.0	41.0
18	37.25234776159849	39.0	35.0	41.0	32.0	41.0
19	37.179162742968316	39.0	35.0	41.0	32.0	41.0
20	37.02969752608904	39.0	35.0	41.0	32.0	41.0
21	36.89496483884709	38.0	35.0	41.0	32.0	41.0
22	36.807213213221104	38.0	35.0	41.0	31.0	41.0
23	36.716220623702775	38.0	35.0	41.0	31.0	41.0
24	36.708424448504225	38.0	35.0	41.0	31.0	41.0
25	36.67146879187741	38.0	35.0	41.0	31.0	41.0
26	36.537196222567495	38.0	35.0	40.0	31.0	41.0
27	36.46697285741992	38.0	35.0	40.0	31.0	41.0
28	36.376545017789624	38.0	35.0	40.0	31.0	41.0
29	36.3036358072443	38.0	35.0	40.0	31.0	41.0
30	36.22310422437294	38.0	35.0	40.0	30.0	41.0
31	36.11653504491951	38.0	35.0	40.0	30.0	41.0
32	35.961042764700245	38.0	35.0	40.0	30.0	41.0
33	35.80484038811013	38.0	35.0	40.0	29.0	41.0
34	35.63160620559435	38.0	35.0	40.0	29.0	41.0
35	35.4518988511061	38.0	34.0	40.0	27.0	41.0
36	35.2701373830716	38.0	34.0	40.0	27.0	41.0
37	35.144840834656556	38.0	34.0	40.0	26.0	41.0
38	35.0520524280524	38.0	34.0	40.0	26.0	41.0
39	34.98467426408493	37.0	34.0	40.0	25.0	41.0
40	34.84713490123663	37.0	34.0	40.0	24.0	41.0
41	34.77783702544045	37.0	34.0	40.0	24.0	41.0
42	34.74806901506306	37.0	34.0	40.0	24.0	41.0
43	34.639930794060234	37.0	34.0	40.0	24.0	41.0
44	34.4674912015032	37.0	33.0	40.0	23.0	41.0
45	34.43496773702093	36.0	34.0	40.0	23.0	41.0
46	34.35577097772215	36.0	33.0	40.0	23.0	41.0
47	34.2637272529471	36.0	33.0	40.0	23.0	41.0
48	34.14012101408066	36.0	33.0	40.0	23.0	41.0
49	34.032366735443574	36.0	33.0	40.0	23.0	41.0
50	33.854924509576016	35.0	33.0	40.0	23.0	41.0
51	33.47327923116964	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	11.0
12	12.0
13	25.0
14	49.0
15	93.0
16	193.0
17	448.0
18	1068.0
19	2148.0
20	3705.0
21	5895.0
22	9113.0
23	12804.0
24	18979.0
25	26784.0
26	35266.0
27	40736.0
28	44980.0
29	49784.0
30	57696.0
31	69141.0
32	85091.0
33	110614.0
34	191708.0
35	284493.0
36	157979.0
37	204858.0
38	313642.0
39	556789.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.102921508083586	23.946796182640988	29.387789231839463	15.562493077435965
2	30.1843930273965	26.14406725864713	30.34326724008481	13.32827247387156
3	29.04044616116736	25.87057946403047	31.91484797502142	13.174126399780755
4	26.034313152499543	27.732021362430647	31.245509910047165	14.988155575022644
5	23.655490310161515	32.26429244062574	29.345235984461937	14.734981264750807
6	21.67291174973087	40.912714621374604	26.586892461552136	10.827481167342397
7	87.37998517640992	3.9687032248094187	6.868890905644302	1.7824206931363626
8	88.02182999102092	3.0267529464402587	6.847482944772276	2.10393411776655
9	83.03211150351743	5.573643604295076	8.394985196110493	2.999259696077002
10	45.96674454961634	29.35425447104606	14.174915736252172	10.504085243085425
11	38.062697744546554	20.683373631959064	25.25434540015594	15.999583223338442
12	35.41235716534082	19.86798861919528	27.635444753670548	17.084209461793357
13	23.915888165512868	30.87448236732646	27.568200115839396	17.641429351321275
14	17.77390479017353	33.80934306454303	29.73942265049818	18.67732949478526
15	16.732926275623335	25.1487940838728	41.00692716083596	17.11135247966791
16	20.198476751348505	22.013118833445628	39.33561772474091	18.452786690464965
17	20.33651213095893	22.030761795064084	29.241567167805577	28.391158906171405
18	22.37604724986505	24.635966162288103	32.42474269951322	20.563243888333623
19	26.334593732502054	25.409410834529595	27.008616157012728	21.24737927595562
20	27.232589833538878	25.276191151638848	27.11359834550172	20.377620669320553
21	23.936420545163138	26.090437908814344	30.02223538512659	19.95090616089593
22	23.335728047974847	23.351006940294557	29.046137439108797	24.267127572621803
23	20.956029624415056	27.28560627651643	28.004764913008817	23.753599186059695
24	21.16494330392694	24.591486636222708	34.22366809867975	20.0199019611706
25	20.53286121993856	26.005988099975614	31.080944419417417	22.38020626066841
26	19.005234662334292	30.73719123175453	27.98882933477279	22.26874477113839
27	19.942062790556157	29.250760770634056	29.82159594816034	20.98558049064945
28	18.025765728612726	27.538868144910445	34.5988984312649	19.83646769521193
29	19.32469047109334	24.662671389551775	33.77462626910025	22.238011870254624
30	20.98290996792308	27.270940291051954	30.967250197771907	20.778899543253054
31	26.19218044678283	25.93270195171432	26.58509752004753	21.29002008145532
32	27.2977330764378	26.42158272688389	26.822029798655723	19.45865439802259
33	25.517326220111485	27.263891862216788	26.49692649101632	20.721855426655406
34	21.485274693907748	26.56241996640395	29.391028882359972	22.56127645732833
35	21.75368411743821	26.2968999609053	29.860778207834088	22.088637713822408
36	26.825663460726023	25.902975969235577	27.465100426977184	19.806260143061216
37	22.2581502383551	29.0931561507173	29.03545534820333	19.613238262724273
38	22.334894932442342	29.429116665506523	25.910812421170327	22.32517598088081
39	21.903058273870425	28.325402756417244	28.092892162978938	21.678646806733397
40	24.092449118880726	25.733901235313766	27.397593114779507	22.776056531026
41	19.43772800682253	25.10899891734382	29.22550025238629	26.227772823447363
42	22.906605691190382	25.91488387385151	26.55090607333781	24.627604361620296
43	22.90713103992344	26.3151120503179	27.106812591033087	23.670944318725574
44	21.067797567372693	28.823520913476376	27.69997508971424	22.40870642943669
45	20.06586997531299	30.956743223110788	25.988607812723686	22.988778988852538
46	22.683726491191432	28.935463972678367	26.897461120910325	21.483348415219876
47	21.94863227646302	26.675632618377488	28.32662857012771	23.049106535031786
48	23.156759246247148	24.910898665920673	29.371459642053644	22.56088244577854
49	22.1801359514963	24.280129953764934	30.078097467074862	23.4616366276639
50	20.424376706562526	28.316165374527678	28.95188112058636	22.307576798323435
51	20.499107563839722	29.245551062364584	26.86716601063744	23.388175363158258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1265.0
1	2553.0
2	3841.0
3	14672.0
4	25503.0
5	18639.5
6	11776.0
7	10960.0
8	10144.0
9	10470.0
10	10796.0
11	10883.0
12	10970.0
13	10686.0
14	10402.0
15	9683.5
16	8965.0
17	8391.0
18	7817.0
19	7986.5
20	8156.0
21	8849.0
22	9542.0
23	10556.5
24	11571.0
25	12642.5
26	17324.5
27	20935.0
28	24778.0
29	28621.0
30	34594.0
31	40567.0
32	44889.0
33	49211.0
34	56402.0
35	63593.0
36	67463.5
37	71334.0
38	77521.5
39	83709.0
40	110111.5
41	136514.0
42	156805.5
43	177097.0
44	177900.5
45	178704.0
46	175228.0
47	171752.0
48	174916.5
49	178081.0
50	174630.5
51	171180.0
52	163347.5
53	155515.0
54	141314.5
55	127114.0
56	118986.0
57	110858.0
58	100753.0
59	90648.0
60	86029.5
61	81411.0
62	72829.5
63	64248.0
64	55507.5
65	46767.0
66	38623.0
67	30479.0
68	26518.0
69	22557.0
70	18594.5
71	14632.0
72	12193.5
73	9755.0
74	8112.5
75	5235.0
76	4000.0
77	2824.0
78	1648.0
79	1341.0
80	1034.0
81	845.0
82	656.0
83	484.0
84	312.0
85	244.0
86	176.0
87	131.5
88	87.0
89	59.5
90	32.0
91	30.0
92	28.0
93	17.0
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2284197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.920374200403696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89727643549514	17.73298571259554
2	6.740799338416627	2.955216878158523
3	2.1749280375144213	1.4302570932379732
4	1.0940970395003828	0.9593206606960901
5	0.7092440912910164	0.7773447940262181
6	0.5004293840468838	0.6581759615511135
7	0.36873917680892077	0.5658030516600254
8	0.32691984468773716	0.5732964263274447
9	0.2667690938244332	0.5262910525560761
>10	4.258537945121131	24.28728044802595
>50	2.1166625908938492	32.84482550534111
>100	0.5403760017081666	15.062122097948953
>500	0.004618595226904787	0.6776126613290988
>1k	4.0161697625259015E-4	0.27564054081088846
>5k	0.0	0.0
>10k+	2.0080848812629507E-4	0.6738271157350815
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15308	0.6701698671349274	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4903	0.21464873651440747	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6267436652793085E-4	0.0	0.0	0.04666847911979571	0.0
2	2.6267436652793085E-4	0.0	0.0	0.14898014488242475	0.0
3	2.6267436652793085E-4	0.0	0.0	0.24940931101827032	0.0
4	2.6267436652793085E-4	0.0	0.0	0.3648984741683839	0.0
5	2.6267436652793085E-4	0.0	0.0	0.6213124349607324	0.0
6	2.6267436652793085E-4	0.0	0.0	0.9811325380429096	0.0
7	2.6267436652793085E-4	0.0	0.0	1.223099408676222	0.0
8	2.6267436652793085E-4	0.0	0.0	1.811183536271171	0.0
9	2.6267436652793085E-4	0.0	0.0	2.080512320084476	0.0
10	2.6267436652793085E-4	0.0	0.0	2.490722122478928	0.0
11	2.6267436652793085E-4	0.0	0.0	2.8277333347342632	0.0
12	3.064534276159193E-4	0.0	0.0	3.10594926794843	0.0
13	3.064534276159193E-4	0.0	0.0	3.235097498157996	0.0
14	3.064534276159193E-4	0.0	0.0	3.288945743296222	0.0
15	3.064534276159193E-4	0.0	0.0	3.355708811455404	0.0
16	3.064534276159193E-4	0.0	0.0	3.489016052468329	0.0
17	3.064534276159193E-4	0.0	0.0	3.6394409063666577	0.0
18	3.064534276159193E-4	0.0	0.0	3.846472086251755	0.0
19	3.064534276159193E-4	0.0	0.0	3.94974689135832	0.0
20	3.064534276159193E-4	0.0	0.0	4.061689950560306	0.0
21	3.064534276159193E-4	0.0	0.0	4.2023958528971015	0.0
22	3.064534276159193E-4	0.0	0.0	4.358993554408836	0.0
23	3.064534276159193E-4	0.0	0.0	4.525966893398424	0.0
24	3.064534276159193E-4	0.0	0.0	4.659799483144405	0.0
25	3.064534276159193E-4	0.0	0.0	4.776251785638454	0.0
26	3.064534276159193E-4	0.0	0.0	4.888282402962616	0.0
27	3.064534276159193E-4	0.0	0.0	5.006485867900185	0.0
28	3.064534276159193E-4	0.0	0.0	5.122237705416827	0.0
29	3.064534276159193E-4	0.0	0.0	5.257120992628919	0.0
30	3.064534276159193E-4	0.0	0.0	5.437840956800136	0.0
31	3.064534276159193E-4	0.0	0.0	5.5828372071235535	0.0
32	3.064534276159193E-4	0.0	0.0	5.7169324712360625	0.0
33	3.064534276159193E-4	0.0	0.0	5.85496785084649	0.0
34	3.064534276159193E-4	0.0	0.0	6.002371949529747	0.0
35	3.064534276159193E-4	0.0	0.0	6.1722347065511425	0.0
36	3.064534276159193E-4	0.0	0.0	6.322090432655327	0.0
37	3.064534276159193E-4	0.0	0.0	6.4760613905017825	0.0
38	3.064534276159193E-4	0.0	0.0	6.653147692602696	0.0
39	3.064534276159193E-4	0.0	0.0	6.880536135893708	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	440	0.0	41.420456	31
CGTTTTT	8250	0.0	41.399998	1
CACGACG	450	0.0	41.0	26
GCGTATG	110	0.0	40.909092	1
TAATGCG	100	0.0	40.5	1
TAGGGAC	1950	0.0	39.80769	5
TACGGGA	730	0.0	39.452057	4
TAAGGGA	2515	0.0	39.274353	4
TATAGCG	75	0.0	39.0	1
ATAGCGG	480	0.0	38.906246	2
AACGGGA	815	0.0	38.650307	4
TAATACG	35	6.250999E-6	38.571426	1
ACGGGTA	140	0.0	38.571426	5
CGACGGT	480	0.0	38.437496	28
ACGGGAT	1180	0.0	38.32627	5
CTATGCG	60	1.5643309E-10	37.499996	1
CGTAAGG	240	0.0	37.499996	2
TTACGGG	745	0.0	37.449665	3
GCGATAC	175	0.0	37.285713	9
ACGGTCT	495	0.0	37.272728	30
>>END_MODULE