FastQCFastQC Report
Thu 26 May 2016
SRR1548038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548038_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2727265
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97180.35632767626174944No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79590.2918308268540094No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA253.892219E-545.00000437
GACCGTA253.892219E-545.0000048
CGCGATA253.892219E-545.00000412
GTATCGC207.034992E-445.00000435
TATCGCC207.034992E-445.00000436
TATAGCG2550.043.2352941
CTCACGA4650.042.58064724
TAGCACG850.042.352941
TCACGAC4550.042.03296725
CGCAATC1300.041.5384620
CGACGGT4750.040.7368428
CGTTTTT62150.040.4022521
ACGTAAG950.040.263161
CGTTAGG4050.039.4444432
GGGCGAT44100.038.979597
CGGTCTA4850.038.96907431
TCGATAG1050.038.571431
TCGAGTA356.2514464E-638.57142639
ATAGGGC11050.038.4841654
ATTAGCG3300.038.1818161
Produced by FastQC (version 0.11.5)