##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548038_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2727265
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.552354465004317	31.0	31.0	33.0	30.0	34.0
2	31.961256790227573	31.0	31.0	34.0	30.0	34.0
3	32.05433428728048	33.0	31.0	34.0	30.0	34.0
4	35.78081924565453	37.0	35.0	37.0	35.0	37.0
5	35.702041789118404	37.0	35.0	37.0	33.0	37.0
6	35.76384583089652	37.0	35.0	37.0	35.0	37.0
7	36.045990030305084	37.0	35.0	37.0	35.0	37.0
8	36.06288937818657	37.0	35.0	37.0	35.0	37.0
9	37.89947328184097	39.0	38.0	39.0	35.0	39.0
10	37.39359138184225	39.0	37.0	39.0	35.0	39.0
11	37.02708097672944	39.0	37.0	39.0	33.0	39.0
12	36.07071076701384	37.0	35.0	39.0	32.0	39.0
13	35.708807908289074	37.0	35.0	39.0	31.0	39.0
14	36.64687993282648	38.0	35.0	41.0	31.0	41.0
15	36.90091245258528	38.0	35.0	41.0	32.0	41.0
16	37.06496838407709	38.0	35.0	41.0	32.0	41.0
17	37.00674411910834	38.0	35.0	41.0	32.0	41.0
18	36.9570368116043	38.0	35.0	41.0	32.0	41.0
19	36.893401264636914	38.0	35.0	41.0	32.0	41.0
20	36.75142202902908	38.0	35.0	41.0	32.0	41.0
21	36.6036413769839	38.0	35.0	41.0	31.0	41.0
22	36.50457656296693	38.0	35.0	41.0	31.0	41.0
23	36.43047265300585	38.0	35.0	40.0	31.0	41.0
24	36.43782984051788	38.0	35.0	40.0	31.0	41.0
25	36.39524578652973	38.0	35.0	40.0	31.0	41.0
26	36.25574669128229	38.0	35.0	40.0	31.0	41.0
27	36.19208034422764	37.0	35.0	40.0	31.0	41.0
28	36.133236410836496	37.0	35.0	40.0	31.0	41.0
29	36.087062313343225	37.0	35.0	40.0	31.0	41.0
30	36.02065622519264	37.0	35.0	40.0	30.0	41.0
31	35.89606217217615	37.0	35.0	40.0	30.0	41.0
32	35.73457071461702	37.0	34.0	40.0	30.0	41.0
33	35.611486965879735	37.0	34.0	40.0	30.0	41.0
34	35.48742494770402	37.0	34.0	40.0	29.0	41.0
35	35.34961692391462	37.0	34.0	40.0	29.0	41.0
36	35.16513173453991	37.0	34.0	40.0	27.0	41.0
37	35.063879014323874	36.0	34.0	40.0	27.0	41.0
38	34.975040929282635	36.0	34.0	40.0	27.0	41.0
39	34.94409050825644	36.0	34.0	40.0	27.0	41.0
40	34.75116426163208	36.0	34.0	40.0	25.0	41.0
41	34.72186567861942	36.0	34.0	40.0	25.0	41.0
42	34.684812073634205	36.0	34.0	40.0	26.0	41.0
43	34.54838602042706	35.0	34.0	40.0	25.0	41.0
44	34.38397258792233	35.0	34.0	40.0	24.0	41.0
45	34.34853048750305	35.0	34.0	40.0	24.0	41.0
46	34.300890819190656	35.0	34.0	40.0	24.0	41.0
47	34.22776591200342	35.0	33.0	40.0	24.0	41.0
48	34.127879028990584	35.0	33.0	40.0	24.0	41.0
49	34.05552082397567	35.0	33.0	40.0	24.0	41.0
50	33.861162739961095	35.0	33.0	39.0	24.0	41.0
51	33.46370484716373	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	13.0
11	4.0
12	13.0
13	28.0
14	62.0
15	117.0
16	242.0
17	641.0
18	1366.0
19	2705.0
20	4681.0
21	7300.0
22	10975.0
23	15806.0
24	22342.0
25	30714.0
26	39088.0
27	45688.0
28	51001.0
29	58002.0
30	67735.0
31	82498.0
32	103984.0
33	138220.0
34	262148.0
35	431735.0
36	174953.0
37	219953.0
38	338731.0
39	616408.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.01150969927748	23.433109727144227	29.64306732202408	12.912313251554213
2	29.863434613064737	25.745719612872236	30.81160063286846	13.579245141194567
3	28.84450172608822	25.260398237795005	31.57767947009183	14.317420566024937
4	25.86264994417484	27.83473553175067	30.86784745890113	15.434767065173352
5	23.56683343936141	33.022460230303984	28.687751281961965	14.722955048372638
6	21.45640412647836	41.06703968994579	26.447338267458427	11.029217916117428
7	88.5560075753548	4.181735181583014	5.50943894341034	1.7528182996518489
8	89.840297880844	2.947165016967548	5.259701569154446	1.9528355330340104
9	85.618192584879	4.95874071643203	6.726079057223995	2.6969876414649843
10	52.42149186089361	25.869946631515457	11.675176412999837	10.033385094591102
11	45.55552907399904	19.811826133507378	20.81708231506656	13.815562477427019
12	41.16860664438549	20.237014004873014	23.434686398278128	15.159692952463367
13	24.328842265053087	36.02550540559865	23.830724187051864	15.814928142296402
14	17.89961738224925	38.94256700393984	27.170406982819785	15.987408630991121
15	16.512696785974228	23.37810957131045	44.896663873880975	15.212529768834345
16	19.676929084632405	20.274084109904976	41.95012219201288	18.098864613449738
17	20.37213105437132	19.6204622579764	27.659798369428714	32.34760831822357
18	24.55434290397156	23.00333850945911	32.26048807138287	20.181830515186462
19	28.75961815225143	25.091767760008654	25.403141975568932	20.745472112170983
20	31.1731716573197	24.57703963494563	24.083981571281118	20.165807136453555
21	24.709480010193364	26.649922174779494	28.01205603415876	20.628541780868378
22	24.74211343598807	23.207865755619643	26.708259006733854	25.341761801658436
23	20.753355467840493	27.623094932102312	26.2729511066948	25.350598493362398
24	22.432986893462864	23.436116402329805	35.23188982368784	18.899006880519494
25	19.734092579928976	25.034677598253197	32.40480848029069	22.826421341527134
26	18.235925001787507	32.29554150403426	26.502265089751088	22.966268404427144
27	19.223874467644325	30.728183729853903	29.47366684205605	20.57427496044572
28	16.474013343037804	28.028996082155565	36.067892192361214	19.429098382445417
29	17.996197655893358	24.420179190507707	35.3473534841682	22.23626966943073
30	20.585494992235812	28.118316335229615	31.4919158937617	19.804272778772873
31	28.783708220506625	25.498328911931917	25.242944855010425	20.475018012551036
32	29.280652961850056	26.56335192949713	26.45694496134406	17.69905014730875
33	27.884932340641633	27.03052325314922	24.949317356399174	20.135227049809977
34	20.601261703574828	28.399477131851874	28.71748803288276	22.281773131690542
35	20.67855525590656	27.243337189455367	29.854634588131333	22.223472966506737
36	28.933198644062823	25.335601784205053	26.712035684101103	19.019163887631017
37	21.619864589616338	30.079585225491474	29.1028924581953	19.197657726696892
38	21.99364564866267	30.622106762635827	23.8268741761435	23.557373412558004
39	22.057775830364854	29.246956199709235	27.08578007637688	21.60948789354903
40	24.699469981831616	25.515415627010945	24.991117474832844	24.793996916324595
41	18.887346847482732	24.464765983503618	27.576161465790822	29.07172570322283
42	23.51234661831542	24.776506866769456	25.278144954744036	26.43300156017109
43	23.43186305694533	25.322181746181617	26.459328301430183	24.786626895442872
44	21.254113553321734	28.74392477445353	27.250707210337094	22.751254461887644
45	19.45821179826676	33.51885497008908	23.951394528951162	23.07153870269299
46	23.47160983622787	30.5727166226971	25.462725477722188	20.492948063352845
47	21.841551884397006	28.222889964854904	26.738912426918542	23.19664572382955
48	23.91564442765921	23.97405459315468	29.998991663809715	22.111309315376392
49	22.86154810771964	23.03593526848326	30.12219201287737	23.98032461091973
50	21.318537069188363	30.081161896625375	26.468861661774707	22.131439372411556
51	19.17338432458892	31.24162851794747	25.47530218002284	24.10968497744077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1376.0
1	2479.5
2	3583.0
3	10789.0
4	17995.0
5	13607.5
6	9220.0
7	9025.5
8	8831.0
9	9124.5
10	9418.0
11	9476.0
12	9534.0
13	9210.0
14	8886.0
15	8817.0
16	8748.0
17	8944.5
18	9141.0
19	8374.5
20	7608.0
21	8232.5
22	8857.0
23	10161.5
24	11466.0
25	11891.0
26	14547.5
27	16779.0
28	21037.0
29	25295.0
30	29970.5
31	34646.0
32	38475.5
33	42305.0
34	52983.0
35	63661.0
36	69121.0
37	74581.0
38	91039.0
39	107497.0
40	140254.5
41	173012.0
42	212342.0
43	251672.0
44	258153.5
45	264635.0
46	253705.0
47	242775.0
48	228631.5
49	214488.0
50	202815.0
51	191142.0
52	179449.0
53	167756.0
54	159651.0
55	151546.0
56	141221.0
57	130896.0
58	122773.0
59	114650.0
60	106080.5
61	97511.0
62	85758.5
63	74006.0
64	63911.5
65	53817.0
66	44533.0
67	35249.0
68	29588.5
69	23928.0
70	20634.5
71	17341.0
72	14752.5
73	12164.0
74	10059.0
75	6196.0
76	4438.0
77	3565.0
78	2692.0
79	2419.0
80	2146.0
81	1508.0
82	870.0
83	623.5
84	377.0
85	315.0
86	253.0
87	179.0
88	105.0
89	81.0
90	57.0
91	38.5
92	20.0
93	16.5
94	13.0
95	10.5
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2727265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.72025267190963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07059711653756	15.36382314956081
2	7.5081352268981645	2.8110837708479837
3	2.3232933350013543	1.304779147865668
4	1.0786470968165687	0.8077018478491174
5	0.6192191773283849	0.5795969729439691
6	0.4248026872465159	0.47714481845765877
7	0.31802306339298325	0.41674304715479654
8	0.23006630634214686	0.34455195088143636
9	0.19571341465141565	0.32974241162010487
>10	2.2827466781217827	10.650042682505907
>50	1.3581547763894952	18.758069517081307
>100	1.5842970302140258	46.71713211485377
>500	0.005713082522762767	0.6990127251191637
>1k	1.970028456125092E-4	0.08865815084871106
>5k	3.940056912250184E-4	0.6519176924095946
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9718	0.35632767626174944	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7959	0.2918308268540094	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.333354111169981E-5	0.0	0.0	0.03223009131859207	0.0
2	7.333354111169981E-5	0.0	0.0	0.09265692919463271	0.0
3	7.333354111169981E-5	0.0	0.0	0.1378303905194398	0.0
4	7.333354111169981E-5	0.0	0.0	0.2194506217767617	0.0
5	7.333354111169981E-5	0.0	0.0	0.3774110693313631	0.0
6	7.333354111169981E-5	0.0	0.0	0.5820849825741173	0.0
7	7.333354111169981E-5	0.0	0.0	0.712618685752943	0.0
8	7.333354111169981E-5	0.0	0.0	1.0960797722260214	0.0
9	7.333354111169981E-5	0.0	0.0	1.2690369289379653	0.0
10	7.333354111169981E-5	0.0	0.0	1.576231132654876	0.0
11	1.1000031166754972E-4	0.0	0.0	1.861278606956053	0.0
12	1.1000031166754972E-4	0.0	0.0	2.099539282027966	0.0
13	1.1000031166754972E-4	0.0	0.0	2.2019862389610103	0.0
14	1.1000031166754972E-4	0.0	0.0	2.2417696850141073	0.0
15	1.1000031166754972E-4	0.0	0.0	2.2997398492629064	0.0
16	1.1000031166754972E-4	0.0	0.0	2.4313368887878517	0.0
17	1.1000031166754972E-4	0.0	0.0	2.580790645573496	0.0
18	1.1000031166754972E-4	0.0	0.0	2.8052279481458533	0.0
19	1.1000031166754972E-4	0.0	0.0	2.910498246411698	0.0
20	1.1000031166754972E-4	0.0	0.0	3.0247152366931704	0.0
21	1.1000031166754972E-4	0.0	0.0	3.1626922942948337	0.0
22	1.1000031166754972E-4	0.0	0.0	3.302392690112622	0.0
23	1.1000031166754972E-4	0.0	0.0	3.4644964827400346	0.0
24	1.1000031166754972E-4	0.0	0.0	3.585826826509342	0.0
25	1.1000031166754972E-4	0.0	0.0	3.6918304601863037	0.0
26	1.1000031166754972E-4	0.0	0.0	3.797137425222705	0.0
27	1.1000031166754972E-4	0.0	0.0	3.902921058276332	0.0
28	1.1000031166754972E-4	0.0	0.0	4.013398037961108	0.0
29	1.1000031166754972E-4	0.0	0.0	4.135681717764867	0.0
30	1.1000031166754972E-4	0.0	0.0	4.291625492938897	0.0
31	1.4666708222339962E-4	0.0	0.0	4.432792559578918	0.0
32	1.4666708222339962E-4	0.0	0.0	4.553902902724891	0.0
33	1.4666708222339962E-4	0.0	0.0	4.686856612760403	0.0
34	1.4666708222339962E-4	0.0	0.0	4.8282436800237605	0.0
35	1.4666708222339962E-4	0.0	0.0	4.998817496649574	0.0
36	1.4666708222339962E-4	0.0	0.0	5.141341233800162	0.0
37	1.4666708222339962E-4	0.0	0.0	5.297175008662524	0.0
38	1.4666708222339962E-4	0.0	0.0	5.478015521043976	0.0
39	1.8333385277924955E-4	0.0	0.0	5.718146201414237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	25	3.892219E-5	45.000004	37
GACCGTA	25	3.892219E-5	45.000004	8
CGCGATA	25	3.892219E-5	45.000004	12
GTATCGC	20	7.034992E-4	45.000004	35
TATCGCC	20	7.034992E-4	45.000004	36
TATAGCG	255	0.0	43.235294	1
CTCACGA	465	0.0	42.580647	24
TAGCACG	85	0.0	42.35294	1
TCACGAC	455	0.0	42.032967	25
CGCAATC	130	0.0	41.53846	20
CGACGGT	475	0.0	40.73684	28
CGTTTTT	6215	0.0	40.402252	1
ACGTAAG	95	0.0	40.26316	1
CGTTAGG	405	0.0	39.444443	2
GGGCGAT	4410	0.0	38.97959	7
CGGTCTA	485	0.0	38.969074	31
TCGATAG	105	0.0	38.57143	1
TCGAGTA	35	6.2514464E-6	38.571426	39
ATAGGGC	1105	0.0	38.484165	4
ATTAGCG	330	0.0	38.181816	1
>>END_MODULE