FastQCFastQC Report
Thu 26 May 2016
SRR1548037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548037_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1884057
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105510.5600149040076813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT950.045.028
GCGAACG207.0344366E-445.01
TTTCGCG253.8917595E-545.01
CGTTTTT54600.041.2912061
CGTAAGG2900.041.120692
CGGTCTA1050.040.71428731
GGCGTAG900.040.01
TCTAGCG900.040.01
GTAACCG403.4597906E-739.3751
TACGGGA9800.038.571434
TAACGCG700.038.571431
AATCGCG356.250413E-638.571431
TTAGGGA30750.038.268294
AACGGGA9000.038.04
TAGACGG2500.037.82
GTTACGG2500.037.82
AGGGCGA15950.037.6645746
TAAGGGA31300.037.5958484
CGTTAGG2700.037.5000042
GGGCGAT30800.037.40267
Produced by FastQC (version 0.11.5)