FastQCFastQC Report
Thu 26 May 2016
SRR1548036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548036_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2273478
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111320.4896462600473811No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26560.11682541023049266No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGT207.034744E-445.00000412
CGTAAGT207.034744E-445.00000421
TACCGCG302.1664418E-645.0000041
CGACGGT950.045.028
TAACGCG502.1827873E-1145.01
TACGCGT351.2126293E-745.024
CGTTTTT62950.041.1397931
TACGGGA10600.040.5424544
ACGCGAG451.9297659E-840.0000041
ACGTACG451.9297659E-840.0000041
GCGTAAG1700.039.7058831
ACGATAC403.460209E-739.37500420
CTACGGG4500.039.03
TAGGGCG7100.038.9788745
CGGTCTA1100.038.86363631
TAGGGAT55500.038.7567565
GTACGCG356.250986E-638.5714261
AGGGCGA19150.038.5378576
GAGGGAT47800.038.31595
GGGCGAT40300.038.2444157
Produced by FastQC (version 0.11.5)