##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548036_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2273478
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8396663614075	31.0	31.0	31.0	30.0	33.0
2	31.17945940097067	31.0	31.0	33.0	30.0	34.0
3	31.320053679868465	31.0	31.0	34.0	30.0	34.0
4	35.206451085077575	35.0	35.0	37.0	33.0	37.0
5	35.03601134473261	35.0	35.0	37.0	32.0	37.0
6	35.07804210113316	37.0	35.0	37.0	32.0	37.0
7	35.535445251724454	35.0	35.0	37.0	35.0	37.0
8	35.641763412709516	37.0	35.0	37.0	35.0	37.0
9	37.462973030748486	39.0	37.0	39.0	35.0	39.0
10	36.54353901819151	39.0	35.0	39.0	32.0	39.0
11	36.077625558725444	38.0	35.0	39.0	31.0	39.0
12	35.68557118212712	37.0	35.0	39.0	30.0	39.0
13	35.46808238302724	37.0	35.0	39.0	30.0	39.0
14	36.488659665939146	38.0	35.0	40.0	30.0	41.0
15	36.6993469917017	38.0	35.0	40.0	31.0	41.0
16	36.79999146681868	38.0	35.0	40.0	31.0	41.0
17	36.7211892967515	38.0	35.0	40.0	31.0	41.0
18	36.639875116451535	38.0	35.0	40.0	31.0	41.0
19	36.586663253394136	38.0	35.0	40.0	31.0	41.0
20	36.46544325478408	38.0	35.0	40.0	30.0	41.0
21	36.35439753540611	38.0	35.0	40.0	30.0	41.0
22	36.23074865910292	38.0	34.0	40.0	30.0	41.0
23	36.15568569390159	38.0	34.0	40.0	30.0	41.0
24	36.05582108118046	38.0	34.0	40.0	30.0	41.0
25	35.90024315168213	38.0	34.0	40.0	29.0	41.0
26	35.67593484520193	38.0	34.0	40.0	29.0	41.0
27	35.73635944574788	38.0	34.0	40.0	29.0	41.0
28	35.77056958545453	38.0	34.0	40.0	29.0	41.0
29	35.79600110491502	38.0	34.0	40.0	29.0	41.0
30	35.731408441163715	38.0	34.0	40.0	29.0	41.0
31	35.63167402543592	38.0	34.0	40.0	29.0	41.0
32	35.49728433703779	38.0	34.0	40.0	28.0	41.0
33	35.35397351546837	38.0	34.0	40.0	27.0	41.0
34	35.22304592347056	38.0	34.0	40.0	27.0	41.0
35	35.08729400504426	38.0	34.0	40.0	26.0	41.0
36	35.00290084179394	38.0	34.0	40.0	25.0	41.0
37	34.95603696187076	38.0	33.0	40.0	25.0	41.0
38	34.923876105244915	38.0	33.0	40.0	25.0	41.0
39	34.912406893754856	38.0	34.0	40.0	25.0	41.0
40	34.75997392541296	37.0	33.0	40.0	24.0	41.0
41	34.70460501487149	37.0	33.0	40.0	24.0	41.0
42	34.67340304150733	37.0	33.0	40.0	24.0	41.0
43	34.63168194282065	37.0	33.0	40.0	24.0	41.0
44	34.50484060105266	37.0	33.0	40.0	24.0	41.0
45	34.382981933407756	37.0	33.0	40.0	23.0	41.0
46	34.32705000884108	37.0	33.0	40.0	23.0	41.0
47	34.28260269067921	37.0	33.0	40.0	23.0	41.0
48	34.217227085549105	37.0	33.0	40.0	23.0	41.0
49	34.091052123662514	36.0	33.0	40.0	24.0	41.0
50	33.9756443651533	36.0	33.0	40.0	23.0	41.0
51	33.6629630900321	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	11.0
14	26.0
15	82.0
16	206.0
17	528.0
18	1201.0
19	2288.0
20	4039.0
21	6387.0
22	9667.0
23	14427.0
24	20527.0
25	28727.0
26	37516.0
27	44168.0
28	50969.0
29	59814.0
30	72737.0
31	87928.0
32	106837.0
33	133441.0
34	192123.0
35	226868.0
36	195081.0
37	238497.0
38	328682.0
39	410658.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.606639694776025	25.117507185026643	30.057823299807605	13.218029820389729
2	29.285702346800807	26.857440450270463	30.608521393213394	13.248335809715334
3	27.92611144686687	26.523854640335205	32.22182048825632	13.328213424541605
4	24.246418922901388	29.17789395806777	31.88154888677172	14.694138232259121
5	22.586759141720307	34.00169256091328	30.139196420638335	13.272351876728077
6	21.305550350608186	42.66555471396688	26.2197830812526	9.809111854172329
7	92.23458507185907	2.36100811180051	4.58970792767733	0.8146988886630967
8	92.97266127052912	1.8047678490840906	4.223177000173303	0.9993938802134879
9	88.39804035930851	4.856567778531396	5.203965026272521	1.5414268358875696
10	39.37398998362861	42.580266886242136	9.805285118219748	8.240458011909507
11	28.157650964733328	25.66160745782453	29.38836443545968	16.79237714198246
12	29.010969096687983	24.470436925274843	28.403397789642128	18.11519618839505
13	22.577302265515655	30.801617609671172	29.315964350655694	17.305115774157482
14	17.718887097214047	34.352916544606984	29.412380502472423	18.515815855706546
15	16.733656538572177	28.687851828783916	37.741205325057024	16.837286307586876
16	20.497493268023707	27.117526538633758	35.70775701370324	16.677223179639302
17	20.43336245171495	25.99048682239283	29.760569488686496	23.815581237205727
18	21.675248232004005	27.549551832038844	32.06496829967126	18.7102316362859
19	24.395045828461942	28.52303826999865	28.04060562714924	19.041310274390163
20	26.19831817154158	28.751894674151234	28.086262545755886	16.963524608551303
21	23.807795808888407	28.168647332413155	30.519890669713977	17.503666188984454
22	23.737287099325354	25.52173366093712	29.82865899735999	20.91232024237754
23	19.975209788702596	30.096354572157725	29.386649002101628	20.541786637038054
24	19.786028279138833	27.928574633227154	34.1078734872297	18.177523600404314
25	19.38611237935885	30.139900188169843	31.290648073128484	19.18333935934282
26	19.33618887009243	34.029095509171405	27.760110280372185	18.87460534036397
27	18.77471433636041	33.15589594445163	30.110693835612224	17.958695883575736
28	17.658099176679958	30.795723556594783	33.72845481680491	17.817722449920343
29	18.13248247838774	28.270385726186927	33.298804738818674	20.29832705660666
30	19.955636254232502	30.22259287312215	31.165509408932042	18.656261463713307
31	22.9386429074748	29.918037473861638	28.322332567106436	18.820987051557132
32	23.84971396248391	29.715396410257767	28.887633836791032	17.547255790467293
33	22.64987829220252	29.90752494635972	28.15448401084154	19.288112750596223
34	19.832828819984183	30.308452511966248	29.375828576304674	20.482890091744895
35	19.78000226965029	29.105493873263782	30.601351761486146	20.513152095599782
36	23.417908596432426	29.27809286036636	28.690402986085633	18.613595557115573
37	19.705490882251773	31.28079532768736	30.182654065709013	18.83105972435185
38	19.53566297980451	31.977349241998382	27.857098243308272	20.629889534888836
39	19.668279174023237	31.00003606808599	29.322518185792866	20.009166572097907
40	21.393169408281057	29.07826686688853	28.80700846896253	20.721555255867884
41	18.695100634358457	28.57071852025839	29.631692059478915	23.102488785904242
42	20.930574212726054	28.94529878890405	28.140848514918552	21.983278483451347
43	20.65953574215365	29.30347247697141	28.61870666881316	21.41828511206178
44	19.944507930140514	30.840984605964955	28.91697214576081	20.297535318133715
45	18.853228401594386	32.997768177215704	27.160148459760773	20.98885496142914
46	21.01194733355678	31.062363480095257	28.018524920848147	19.90716426549982
47	19.794869358753417	30.130267370082315	28.999796787125277	21.07506648403899
48	20.453111928067923	28.980487165479502	30.426245602552566	20.140155303900016
49	20.246336230216436	28.07975269608943	30.285668038133647	21.388243035560496
50	19.116525429320184	31.336744846442322	29.27593757230112	20.270792151936373
51	18.61746627853887	31.460651917458627	28.08028052173806	21.841601282264442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1818.0
1	1923.0
2	2028.0
3	10199.5
4	18371.0
5	13935.0
6	9499.0
7	9074.0
8	8649.0
9	9735.5
10	10822.0
11	11696.0
12	12570.0
13	12761.0
14	12952.0
15	12943.0
16	12934.0
17	12713.5
18	12493.0
19	12660.5
20	12828.0
21	13550.0
22	14272.0
23	16993.0
24	19714.0
25	22036.0
26	28914.0
27	33470.0
28	40273.5
29	47077.0
30	55897.5
31	64718.0
32	73463.5
33	82209.0
34	87428.5
35	92648.0
36	100858.0
37	109068.0
38	116238.0
39	123408.0
40	139256.5
41	155105.0
42	171471.0
43	187837.0
44	190755.5
45	193674.0
46	189588.5
47	185503.0
48	182345.5
49	179188.0
50	165401.5
51	151615.0
52	137266.5
53	122918.0
54	109331.5
55	95745.0
56	88145.5
57	80546.0
58	71933.5
59	63321.0
60	56094.5
61	48868.0
62	41211.0
63	33554.0
64	27302.5
65	21051.0
66	16563.5
67	12076.0
68	9381.5
69	6687.0
70	5365.0
71	4043.0
72	3152.5
73	2262.0
74	2055.5
75	1397.0
76	945.0
77	654.5
78	364.0
79	264.0
80	164.0
81	140.5
82	117.0
83	98.0
84	79.0
85	55.5
86	32.0
87	26.0
88	20.0
89	12.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2273478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.24912777489553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58220980632667	16.27305161458493
2	7.342371743810873	3.120379907100395
3	2.781412199390417	1.7730774965850054
4	1.5877483372830046	1.3495306917321794
5	0.9616476866390661	1.0217087283913107
6	0.740731662754757	0.9443941049271992
7	0.5644296053647029	0.83955457630299
8	0.462950464473903	0.7869834858442559
9	0.40019156351208623	0.7653349500753185
>10	6.1262406849046	32.79277459208592
>50	2.1762926926319253	31.880460231420848
>100	0.2725001898396073	7.67393437431991
>500	6.366815341979355E-4	0.09357508029861177
>1k	4.24454356131957E-4	0.18322677168831789
>5k	0.0	0.0
>10k+	2.122271780659785E-4	0.502013394642962
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11132	0.4896462600473811	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2656	0.11682541023049266	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023444255893393294	0.0
2	0.0	0.0	0.0	0.08088928065281477	0.0
3	0.0	0.0	0.0	0.12170779748033629	0.0
4	0.0	0.0	0.0	0.2024211362502738	0.0
5	0.0	0.0	0.0	0.3787588883639956	0.0
6	0.0	0.0	0.0	0.6223064397368261	0.0
7	0.0	0.0	0.0	0.7603768323247465	0.0
8	0.0	0.0	0.0	1.0444789877007827	0.0
9	0.0	0.0	0.0	1.1574776619786953	0.0
10	0.0	0.0	0.0	1.3418207697633318	0.0
11	0.0	0.0	0.0	1.5784626022332304	0.0
12	0.0	0.0	0.0	1.770855051159501	0.0
13	0.0	0.0	0.0	1.873781052642691	0.0
14	0.0	0.0	0.0	1.9292907166904627	0.0
15	0.0	0.0	0.0	1.9943892133550445	0.0
16	0.0	0.0	0.0	2.113149984297187	0.0
17	0.0	0.0	0.0	2.2698702164700957	0.0
18	0.0	0.0	0.0	2.4342439205481647	0.0
19	0.0	0.0	0.0	2.548914042713411	0.0
20	0.0	0.0	0.0	2.6671030025362024	0.0
21	0.0	0.0	0.0	2.8190728038714252	0.0
22	0.0	0.0	0.0	3.006934749313607	0.0
23	0.0	0.0	0.0	3.2042535709604403	0.0
24	0.0	0.0	0.0	3.357586921887962	0.0
25	0.0	0.0	0.0	3.4921384768183374	0.0
26	4.3985470719312E-5	0.0	0.0	3.6277896685166957	0.0
27	4.3985470719312E-5	0.0	0.0	3.768455203877055	0.0
28	4.3985470719312E-5	0.0	0.0	3.9148828359016448	0.0
29	4.3985470719312E-5	0.0	0.0	4.083831028934522	0.0
30	4.3985470719312E-5	0.0	0.0	4.278070867631004	0.0
31	4.3985470719312E-5	0.0	0.0	4.460434629233272	0.0
32	4.3985470719312E-5	0.0	0.0	4.6361565847569235	0.0
33	4.3985470719312E-5	0.0	0.0	4.809503324861732	0.0
34	4.3985470719312E-5	0.0	0.0	4.990767449696016	0.0
35	4.3985470719312E-5	0.0	0.0	5.195387859482256	0.0
36	4.3985470719312E-5	0.0	0.0	5.3880442212328425	0.0
37	4.3985470719312E-5	0.0	0.0	5.588046156593554	0.0
38	4.3985470719312E-5	0.0	0.0	5.805158440064078	0.0
39	4.3985470719312E-5	0.0	0.0	6.060977937767596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAGT	20	7.034744E-4	45.000004	12
CGTAAGT	20	7.034744E-4	45.000004	21
TACCGCG	30	2.1664418E-6	45.000004	1
CGACGGT	95	0.0	45.0	28
TAACGCG	50	2.1827873E-11	45.0	1
TACGCGT	35	1.2126293E-7	45.0	24
CGTTTTT	6295	0.0	41.139793	1
TACGGGA	1060	0.0	40.542454	4
ACGCGAG	45	1.9297659E-8	40.000004	1
ACGTACG	45	1.9297659E-8	40.000004	1
GCGTAAG	170	0.0	39.705883	1
ACGATAC	40	3.460209E-7	39.375004	20
CTACGGG	450	0.0	39.0	3
TAGGGCG	710	0.0	38.978874	5
CGGTCTA	110	0.0	38.863636	31
TAGGGAT	5550	0.0	38.756756	5
GTACGCG	35	6.250986E-6	38.571426	1
AGGGCGA	1915	0.0	38.537857	6
GAGGGAT	4780	0.0	38.3159	5
GGGCGAT	4030	0.0	38.244415	7
>>END_MODULE