FastQCFastQC Report
Thu 26 May 2016
SRR1548035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548035_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1873032
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58030.30981851885071904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG800.042.18751
CGCATAA451.929402E-840.00000428
CGTTGAT1800.040.00000425
CGATACG451.929402E-840.00000410
CGTTTTT40050.039.8876381
CGTAGAC403.4597724E-739.37510
AATCGCG800.039.3751
TACGGGA8800.038.6079564
TTACCCG356.2503987E-638.57142629
GGGCGAT28950.038.549227
CTCACGA2400.038.43749624
CGAGGGA14350.038.4146354
CGACGGT2350.038.2978728
TTAGGGA28850.038.2928964
ACGGGAT8200.038.1402445
TAGGGAT35400.037.881365
CACGACC7450.037.7516827
CGACAAT2750.037.63636420
CCGACGT301.14019655E-437.49999611
ATTGGGA20750.037.1927764