##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548035_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1873032
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.898416044146604	31.0	31.0	31.0	30.0	33.0
2	31.240592258968345	31.0	31.0	33.0	30.0	34.0
3	31.436228532133995	31.0	31.0	34.0	30.0	34.0
4	35.31558510479266	37.0	35.0	37.0	33.0	37.0
5	35.14186997339074	37.0	35.0	37.0	32.0	37.0
6	35.17622923687369	37.0	35.0	37.0	32.0	37.0
7	35.521682491276174	35.0	35.0	37.0	35.0	37.0
8	35.60174679343439	37.0	35.0	37.0	35.0	37.0
9	37.424422006671534	39.0	37.0	39.0	35.0	39.0
10	36.596519440137705	39.0	35.0	39.0	32.0	39.0
11	36.260544934630055	38.0	35.0	39.0	31.0	39.0
12	35.709836778015536	37.0	35.0	39.0	30.0	39.0
13	35.45351227314856	37.0	35.0	39.0	30.0	39.0
14	36.46106633522545	38.0	35.0	40.0	30.0	41.0
15	36.669004587214744	38.0	35.0	40.0	31.0	41.0
16	36.7857933019831	38.0	35.0	40.0	31.0	41.0
17	36.71562632138693	38.0	35.0	40.0	31.0	41.0
18	36.62836246257405	38.0	35.0	40.0	31.0	41.0
19	36.590584143783985	38.0	35.0	40.0	31.0	41.0
20	36.475449965617244	38.0	35.0	40.0	30.0	41.0
21	36.34388200521934	38.0	35.0	40.0	30.0	41.0
22	36.218855844427644	38.0	34.0	40.0	30.0	41.0
23	36.12820443003643	38.0	34.0	40.0	30.0	41.0
24	36.02462424560819	38.0	34.0	40.0	30.0	41.0
25	35.860196729153586	38.0	34.0	40.0	29.0	41.0
26	35.622644460959556	38.0	34.0	40.0	29.0	41.0
27	35.68892469536025	38.0	34.0	40.0	29.0	41.0
28	35.73313376386522	38.0	34.0	40.0	29.0	41.0
29	35.72403674897172	38.0	34.0	40.0	29.0	41.0
30	35.65862889689018	38.0	34.0	40.0	29.0	41.0
31	35.56459313028288	38.0	34.0	40.0	29.0	41.0
32	35.43378169726945	38.0	34.0	40.0	28.0	41.0
33	35.31427119237685	38.0	34.0	40.0	27.0	41.0
34	35.16369127703104	38.0	34.0	40.0	26.0	41.0
35	35.04285404627363	38.0	34.0	40.0	26.0	41.0
36	34.959090928505226	37.0	33.0	40.0	25.0	41.0
37	34.903024080741815	37.0	33.0	40.0	25.0	41.0
38	34.82912464923184	37.0	33.0	40.0	25.0	41.0
39	34.79064586189665	37.0	33.0	40.0	25.0	41.0
40	34.60218191680654	37.0	33.0	40.0	24.0	41.0
41	34.508430181651995	37.0	33.0	40.0	24.0	41.0
42	34.46631664595159	37.0	33.0	40.0	24.0	41.0
43	34.42057156524822	37.0	33.0	40.0	24.0	41.0
44	34.243593275501965	37.0	33.0	40.0	23.0	41.0
45	34.12901968572881	36.0	33.0	40.0	23.0	41.0
46	34.061085982513916	36.0	33.0	40.0	23.0	41.0
47	34.00733569955025	36.0	33.0	40.0	23.0	41.0
48	33.93700908473534	36.0	33.0	40.0	23.0	41.0
49	33.7946943778857	36.0	32.0	40.0	23.0	41.0
50	33.68385377292006	36.0	32.0	40.0	23.0	41.0
51	33.341166621819596	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	5.0
14	21.0
15	73.0
16	201.0
17	557.0
18	1137.0
19	2181.0
20	3628.0
21	5765.0
22	8780.0
23	12382.0
24	17331.0
25	23915.0
26	30225.0
27	36328.0
28	42402.0
29	50439.0
30	60041.0
31	72630.0
32	89211.0
33	111021.0
34	162998.0
35	188334.0
36	163711.0
37	198837.0
38	269520.0
39	321317.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.76067894195081	25.250556317243912	29.147072767576848	12.841691973228434
2	29.502005304767888	27.40124034186282	29.347282908140382	13.749471445228911
3	28.816005279140988	27.094838742744386	30.647047140678858	13.442108837435773
4	25.778417026511026	29.72613388345741	30.05501240768978	14.440436682341787
5	23.87903677032747	33.21801229236874	28.647508424842716	14.255442512461078
6	21.842979724852537	43.02078127869679	24.863750325675163	10.272488670775513
7	91.5778801429981	3.049333914209688	4.343812599037283	1.0289733437549384
8	92.10611457786092	2.5855938392937228	3.920595056571377	1.3876965262739773
9	86.81501437241862	5.879184124990924	5.401295866808469	1.9045056357819834
10	38.318672612107	41.143504222031446	10.580331782906004	9.95749138295555
11	35.1936859594497	25.502554147499882	23.49356551302914	15.810194380021269
12	33.45212468340103	23.62121949865245	25.413607455718857	17.51304836222766
13	25.046341973869108	30.842345459127234	25.99240162474533	18.118910942258328
14	19.6023879997779	32.87439830179089	27.595470872894857	19.92774282553635
15	18.657022410722295	27.91687488521285	35.19000209286334	18.23610061120152
16	23.125605969358773	25.99816767679356	33.64272473721751	17.233501616630146
17	22.681513182903444	25.856098561049677	26.500401488068544	24.961986767978335
18	23.3343050198822	26.64919766453536	29.8401735795224	20.176323736060034
19	26.994626893720984	27.71799947891974	25.42700818779391	19.860365439565367
20	29.009168022756686	27.322971524245183	25.591661007393363	18.076199445604775
21	26.23062499733053	27.47368971806141	28.10902323078303	18.186662053825028
22	25.744568165413085	24.744958975607464	27.02975709971853	22.48071575926092
23	22.410348568524192	28.666515040853547	27.374011762746182	21.549124627876086
24	21.946982219203942	26.711716617762004	31.77826112954824	19.563040033485812
25	22.004375792832157	28.589901293731234	29.312473038367738	20.09324987506887
26	21.59621405293663	31.918408227942713	25.634692840271818	20.850684878848842
27	20.837337536144602	31.36769686796595	27.784842971182556	20.01012262470689
28	18.943029270188656	30.587304434734698	30.759912270585875	19.70975402449077
29	20.03548257584494	28.046237330702304	30.288057011305735	21.630223082147022
30	21.24416454176971	29.50435443708383	29.1560421818741	20.09543883927237
31	25.0866509488359	28.780875073143434	25.922995442683305	20.209478535337357
32	25.674628089642887	28.33373909255154	26.749623071042034	19.242009746763536
33	24.639354800131553	28.757170192500713	25.392678822358615	21.210796185009116
34	20.963763566239123	29.244561758688587	27.723018079776534	22.068656595295757
35	20.756506028727753	28.90767482883368	28.43838225935275	21.89743688308582
36	25.2832840015547	28.616969704735425	25.965600160595226	20.13414613311465
37	21.66812953542705	30.86001734086764	27.036697717924735	20.435155405780574
38	21.902081758346895	31.07426888595603	25.55039102375186	21.47325833194521
39	21.503583494569234	29.85047772809007	26.533502897975048	22.11243587936565
40	24.17556133584477	27.654039012681043	26.543913825284353	21.626485826189835
41	19.714238731639394	27.687033643845915	27.79194375750121	24.806783867013486
42	22.217773107987476	27.72360536285552	26.673169492032173	23.385452037124832
43	21.863321075133793	27.533699370859654	27.004877652917834	23.598101901088718
44	20.98597354449897	29.16068705713517	28.179710757744665	21.673628640621196
45	20.764194098125394	30.689491690478327	25.450446121582548	23.095868089813735
46	21.73032815242879	30.299962840997914	26.299390506942753	21.670318499630543
47	21.54415941639011	28.69267583255385	27.698031854234205	22.065132896821837
48	22.157603287076782	28.520708669152476	28.315212980878062	21.00647506289268
49	21.949758466486426	27.04251715934378	29.118829790414686	21.888894583755107
50	21.077803262304116	30.30386026506755	27.295636166386906	21.322700306241433
51	20.311345454856085	30.020042369804678	26.81374370539318	22.854868469946055
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1937.0
1	2112.5
2	2288.0
3	6626.0
4	10964.0
5	8578.5
6	6193.0
7	6156.0
8	6119.0
9	6921.5
10	7724.0
11	8236.0
12	8748.0
13	8824.0
14	8900.0
15	8588.0
16	8276.0
17	8442.5
18	8609.0
19	9111.5
20	9614.0
21	9788.0
22	9962.0
23	10481.0
24	11000.0
25	13286.0
26	19470.0
27	23368.0
28	26321.0
29	29274.0
30	32419.5
31	35565.0
32	43298.5
33	51032.0
34	56872.0
35	62712.0
36	68249.5
37	73787.0
38	78669.5
39	83552.0
40	97037.5
41	110523.0
42	121815.5
43	133108.0
44	138226.5
45	143345.0
46	141261.0
47	139177.0
48	138964.5
49	138752.0
50	133375.5
51	127999.0
52	121109.0
53	114219.0
54	105958.5
55	97698.0
56	92405.5
57	87113.0
58	82158.5
59	77204.0
60	72129.5
61	67055.0
62	59500.5
63	51946.0
64	44798.5
65	37651.0
66	30955.0
67	24259.0
68	20367.0
69	16475.0
70	13833.5
71	11192.0
72	9205.0
73	7218.0
74	6367.5
75	4562.5
76	3608.0
77	2637.5
78	1667.0
79	1348.0
80	1029.0
81	806.0
82	583.0
83	408.0
84	233.0
85	197.0
86	161.0
87	114.5
88	68.0
89	46.0
90	24.0
91	17.0
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1873032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.04146059074566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.12746890706593	16.439160580627348
2	6.462370622881652	2.7195543356831355
3	2.3388277634261	1.4763705663801632
4	1.3236781958505925	1.1140849037127825
5	0.9033777860862877	0.9504194042244842
6	0.6607435895851735	0.8341806120506554
7	0.522664196623522	0.7698332666813307
8	0.44428345567208305	0.7478698258915547
9	0.37798025214175474	0.7157930921568758
>10	6.399299812503087	34.22470766933066
>50	2.146609661548348	31.274923616911092
>100	0.2895874831282357	7.939495906570378
>500	0.0020721823248577654	0.2607384214974997
>1k	7.770683718216619E-4	0.2165916569880986
>5k	2.5902279060722067E-4	0.31627614129389436
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5803	0.30981851885071904	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02007440342717049	0.0
2	0.0	0.0	0.0	0.07116803129898475	0.0
3	0.0	0.0	0.0	0.11035582947862076	0.0
4	0.0	0.0	0.0	0.20352028155418594	0.0
5	0.0	0.0	0.0	0.3483122552097348	0.0
6	0.0	0.0	0.0	0.6314894780227994	0.0
7	0.0	0.0	0.0	0.7501740493488632	0.0
8	0.0	0.0	0.0	1.039651217918327	0.0
9	0.0	0.0	0.0	1.1518756753755408	0.0
10	0.0	0.0	0.0	1.333826651119682	0.0
11	0.0	0.0	0.0	1.5717830768507959	0.0
12	0.0	0.0	0.0	1.7446578595560567	0.0
13	0.0	0.0	0.0	1.8428943018592314	0.0
14	0.0	0.0	0.0	1.8969777344967944	0.0
15	0.0	0.0	0.0	1.9531433525962183	0.0
16	0.0	0.0	0.0	2.0593348111511176	0.0
17	0.0	0.0	0.0	2.181222744726198	0.0
18	0.0	0.0	0.0	2.3151766761059074	0.0
19	0.0	0.0	0.0	2.4037496422912157	0.0
20	0.0	0.0	0.0	2.504121659427068	0.0
21	0.0	0.0	0.0	2.62825194657646	0.0
22	0.0	0.0	0.0	2.7639677271931284	0.0
23	0.0	0.0	0.0	2.917835893887558	0.0
24	0.0	0.0	0.0	3.039670438091821	0.0
25	5.33893708169428E-5	0.0	0.0	3.1466627372089744	0.0
26	5.33893708169428E-5	0.0	0.0	3.2512525146393654	0.0
27	5.33893708169428E-5	0.0	0.0	3.3652388213335382	0.0
28	5.33893708169428E-5	0.0	0.0	3.4786378449487247	0.0
29	5.33893708169428E-5	0.0	0.0	3.6127519444408853	0.0
30	5.33893708169428E-5	0.0	0.0	3.7730802250041644	0.0
31	5.33893708169428E-5	0.0	0.0	3.9080485544293957	0.0
32	5.33893708169428E-5	0.0	0.0	4.043283830708712	0.0
33	5.33893708169428E-5	0.0	0.0	4.183644486586455	0.0
34	5.33893708169428E-5	0.0	0.0	4.323417859385211	0.0
35	5.33893708169428E-5	0.0	0.0	4.4941570672577935	0.0
36	5.33893708169428E-5	0.0	0.0	4.639749881475597	0.0
37	5.33893708169428E-5	0.0	0.0	4.804295922333415	0.0
38	5.33893708169428E-5	0.0	0.0	4.981014739737495	0.0
39	5.33893708169428E-5	0.0	0.0	5.252019186004297	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	80	0.0	42.1875	1
CGCATAA	45	1.929402E-8	40.000004	28
CGTTGAT	180	0.0	40.000004	25
CGATACG	45	1.929402E-8	40.000004	10
CGTTTTT	4005	0.0	39.887638	1
CGTAGAC	40	3.4597724E-7	39.375	10
AATCGCG	80	0.0	39.375	1
TACGGGA	880	0.0	38.607956	4
TTACCCG	35	6.2503987E-6	38.571426	29
GGGCGAT	2895	0.0	38.54922	7
CTCACGA	240	0.0	38.437496	24
CGAGGGA	1435	0.0	38.414635	4
CGACGGT	235	0.0	38.29787	28
TTAGGGA	2885	0.0	38.292896	4
ACGGGAT	820	0.0	38.140244	5
TAGGGAT	3540	0.0	37.88136	5
CACGACC	745	0.0	37.75168	27
CGACAAT	275	0.0	37.636364	20
CCGACGT	30	1.14019655E-4	37.499996	11
ATTGGGA	2075	0.0	37.192776	4
>>END_MODULE