FastQCFastQC Report
Thu 26 May 2016
SRR1548034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548034_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2371204
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55830.23545000767542565No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41060.17316097644909506No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC207.0348056E-445.030
TACGACG302.1664819E-644.9999961
CGTAAGG5550.042.9729732
GTATGCG1000.042.751
CGACGGT5000.042.328
CGGTCTA5100.041.4705931
CAATCGA501.0822987E-940.515
GGCGATA10300.039.7572828
TGCGTAG2100.039.6428571
TACGGGA9900.039.318184
TTGGGAC16600.039.1716845
CGGGATA5050.038.7623756
ACGGGAT11350.038.6563875
TCGTTAG700.038.5714261
TCGAATT356.251099E-638.57142611
CACGACG5500.038.45454426
GCGATAT2050.038.4146359
TAGGGTA8800.038.3522725
TTAGGGA36400.038.2623634
AGGGCGA20000.038.1374976
Produced by FastQC (version 0.11.5)