##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1548034_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2371204 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91954677876724 31.0 31.0 33.0 30.0 33.0 2 31.277922523747428 31.0 31.0 33.0 30.0 34.0 3 31.489382609003695 31.0 31.0 34.0 30.0 34.0 4 35.347623401445006 37.0 35.0 37.0 33.0 37.0 5 35.15496768730147 37.0 35.0 37.0 32.0 37.0 6 35.19184093819005 37.0 35.0 37.0 32.0 37.0 7 35.54549334430947 35.0 35.0 37.0 35.0 37.0 8 35.6262873207029 37.0 35.0 37.0 35.0 37.0 9 37.45364211598833 39.0 37.0 39.0 35.0 39.0 10 36.62313955273355 39.0 35.0 39.0 32.0 39.0 11 36.251699136809826 38.0 35.0 39.0 32.0 39.0 12 35.446819843421316 37.0 35.0 39.0 30.0 39.0 13 34.980197401826246 37.0 34.0 39.0 27.0 39.0 14 35.97477230976331 38.0 34.0 40.0 29.0 41.0 15 36.26566166386359 38.0 35.0 40.0 30.0 41.0 16 36.45912034561345 38.0 35.0 40.0 31.0 41.0 17 36.40080018421022 38.0 35.0 40.0 31.0 41.0 18 36.35841412210843 38.0 35.0 40.0 30.0 41.0 19 36.29700396929155 38.0 35.0 40.0 30.0 41.0 20 36.173788084028196 38.0 34.0 40.0 30.0 41.0 21 36.0222574692013 38.0 34.0 40.0 30.0 41.0 22 35.872784458865624 38.0 34.0 40.0 30.0 41.0 23 35.84978643760722 37.0 34.0 40.0 30.0 41.0 24 35.71797154525718 37.0 34.0 40.0 29.0 41.0 25 35.57137808471983 37.0 34.0 40.0 29.0 41.0 26 35.31620012449372 37.0 34.0 40.0 28.0 41.0 27 35.391094566304716 37.0 34.0 40.0 29.0 41.0 28 35.434044476983004 37.0 34.0 40.0 29.0 41.0 29 35.470412077577464 37.0 34.0 40.0 29.0 41.0 30 35.419870243133865 37.0 34.0 40.0 29.0 41.0 31 35.31539631343402 37.0 34.0 40.0 29.0 41.0 32 35.200441632183484 37.0 34.0 40.0 28.0 41.0 33 35.08293466104139 37.0 34.0 40.0 27.0 41.0 34 34.938031059326825 37.0 34.0 40.0 26.0 41.0 35 34.83905602385961 37.0 33.0 40.0 26.0 41.0 36 34.75233467892261 37.0 33.0 40.0 25.0 41.0 37 34.70541927223469 37.0 33.0 40.0 25.0 41.0 38 34.69386438281987 37.0 33.0 40.0 26.0 41.0 39 34.682417455436145 36.0 33.0 40.0 25.0 41.0 40 34.48023493550112 36.0 33.0 40.0 24.0 41.0 41 34.4217536745046 36.0 33.0 40.0 24.0 41.0 42 34.39016297205976 36.0 33.0 40.0 24.0 41.0 43 34.356044439871056 36.0 33.0 40.0 24.0 41.0 44 34.20599450743167 36.0 33.0 40.0 24.0 41.0 45 34.064942113795354 35.0 33.0 40.0 23.0 41.0 46 33.99192182536804 35.0 33.0 40.0 23.0 41.0 47 33.967234788740235 35.0 33.0 40.0 23.0 41.0 48 33.9137792446369 35.0 33.0 40.0 23.0 41.0 49 33.80767998029693 35.0 33.0 39.0 24.0 41.0 50 33.667712267691854 35.0 32.0 39.0 23.0 41.0 51 33.31269894956318 35.0 32.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 7.0 13 11.0 14 22.0 15 113.0 16 246.0 17 663.0 18 1381.0 19 2654.0 20 4545.0 21 7335.0 22 10859.0 23 15798.0 24 22178.0 25 30009.0 26 38377.0 27 46788.0 28 54876.0 29 65542.0 30 79140.0 31 96103.0 32 118440.0 33 151563.0 34 236109.0 35 276755.0 36 188268.0 37 230442.0 38 313478.0 39 379453.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.29916110128019 25.00813932500114 30.56641267474245 12.126286898976216 2 28.55359555736242 27.5743883697902 30.463553536515626 13.408462536331752 3 28.70347722085489 26.75969676164514 30.141227831936856 14.395598185563117 4 25.5972915025447 29.184329985948064 30.05840071119988 15.159977800307352 5 23.319419164272663 33.85988721341563 28.61310119247437 14.207592429837332 6 22.355014583308733 42.618433504666825 24.887778529388445 10.138773382635994 7 91.55812827576202 3.4770099915485972 3.9060747198469636 1.058787012842421 8 92.21589538479186 2.6281585219997945 3.620354891439117 1.5355912017692277 9 87.10536925544997 6.002182857316368 4.898692815970283 1.993755071263375 10 43.197464241794464 38.33988977751387 9.274402371116109 9.188243609575556 11 42.453917925239665 21.993341779113056 20.42300029858249 15.129739997064782 12 39.88450593032063 20.34076359520311 23.508943136060836 16.26578733841542 13 25.904435046499586 33.08875997172744 23.39714339213328 17.609661589639693 14 18.88032408852212 36.606846142297336 25.709049073803858 18.803780695376695 15 17.64584573912662 25.47828023232079 40.26401777324937 16.611856255303213 16 21.131627645702352 21.965718681311266 39.397242919630706 17.50541075335568 17 20.703575061445576 22.664561969362403 26.41189876535296 30.219964203839062 18 22.97250679401688 25.709006901135457 30.430026265137876 20.888460039709784 19 28.43247565371853 27.129213682163154 24.16797542514267 20.270335238975644 20 32.086273471198595 25.067391924102694 24.490090266379443 18.356244338319268 21 26.401861670273835 27.301446859907458 26.6585667028227 19.638124766996008 22 25.52007334670488 24.849485746481534 25.38187351235912 24.24856739445446 23 22.564191018571155 29.688546409334666 24.975118125644187 22.77214444644999 24 22.502323714028822 24.62483194191643 33.384938621898414 19.48790572215634 25 21.4171366107682 25.739539913056824 30.788662637208773 22.054660838966196 26 20.511647247558624 33.28583285115916 24.946440711132404 21.256079190149816 27 20.264937137420482 32.52980342475806 26.890178997673758 20.315080440147707 28 17.881675300817644 29.860779587078966 33.463970202479416 18.793574909623974 29 20.114886783254416 25.981189302987005 32.544943412713536 21.35898050104504 30 21.391411283044395 29.176317178952125 28.82409105247798 20.608180485525494 31 27.314646905116557 27.45381671083551 24.899502531203556 20.33203385284438 32 28.73059424663589 26.682267742463324 26.105472156760868 18.481665854139923 33 27.433194275988065 27.77647979676148 24.71331020021896 20.0770157270315 34 20.741235254326494 28.305957648519488 27.579069535982565 23.373737561171456 35 21.280623683158428 26.249618337350984 29.146037203041153 23.323720776449434 36 26.795121803100873 26.69247352821604 26.52867488415168 19.983729784531405 37 22.277332528116517 31.061646319759916 27.58531109090572 19.075710061217848 38 21.763205527655995 31.04667502247803 23.881876042719227 23.30824340714675 39 21.83877894942822 30.17631549204539 26.41248918271056 21.57241637581583 40 24.622217236475645 26.15709150288208 25.876854121366193 23.34383713927608 41 18.957542244361935 25.30769178864408 27.98662620339709 27.74813976359689 42 22.020922704246452 25.949897183034444 25.672147988954137 26.357032123764974 43 21.87795735837153 27.206895737355367 26.099061911164117 24.816084993108987 44 20.609656528919484 28.329236961476113 28.241855192551967 22.819251317052434 45 20.46023876477941 32.10875150345563 24.408654843699658 23.0223548880653 46 22.635926727519013 30.668976604290478 25.45516961003777 21.239927058152734 47 21.836248589324242 27.431802577930874 26.54735737625274 24.184591456492143 48 22.542219058334922 24.700742745035857 30.330667458388227 22.426370738240994 49 22.110244415916977 24.36163231843401 30.14021568789526 23.38790757775375 50 20.130701533904293 29.623853536009552 27.859222572161652 22.3862223579245 51 18.59991801633263 30.635618023586332 26.03580290856459 24.728661051516447 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2157.0 1 2672.0 2 3187.0 3 7370.5 4 11554.0 5 9154.0 6 6754.0 7 6613.0 8 6472.0 9 6893.0 10 7314.0 11 7651.0 12 7988.0 13 8127.0 14 8266.0 15 8317.0 16 8368.0 17 8308.5 18 8249.0 19 8235.0 20 8221.0 21 8890.5 22 9560.0 23 10385.5 24 11211.0 25 12565.0 26 16453.5 27 18988.0 28 23754.5 29 28521.0 30 34614.0 31 40707.0 32 47788.0 33 54869.0 34 59163.5 35 63458.0 36 68534.0 37 73610.0 38 82134.0 39 90658.0 40 118407.5 41 146157.0 42 168653.5 43 191150.0 44 198280.0 45 205410.0 46 198999.5 47 192589.0 48 186801.0 49 181013.0 50 173157.5 51 165302.0 52 156935.5 53 148569.0 54 137470.0 55 126371.0 56 120718.0 57 115065.0 58 111861.0 59 108657.0 60 104011.0 61 99365.0 62 88995.0 63 78625.0 64 63931.0 65 49237.0 66 39615.5 67 29994.0 68 24409.0 69 18824.0 70 15502.5 71 12181.0 72 9802.5 73 7424.0 74 5961.0 75 3795.0 76 3092.0 77 2380.5 78 1669.0 79 1407.0 80 1145.0 81 804.5 82 464.0 83 331.0 84 198.0 85 147.5 86 97.0 87 67.5 88 38.0 89 31.5 90 25.0 91 19.0 92 13.0 93 7.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2371204.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.406649078484598 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25242041592723 13.969257198790583 2 7.222158484851667 2.514271566700259 3 2.3236977936299774 1.2134337617449782 4 1.1237300867144688 0.782415011134953 5 0.6567637906983517 0.5716028416070758 6 0.4430776367564945 0.46274981625267403 7 0.3254795636870385 0.396585598312299 8 0.2691283994528124 0.3747698885063465 9 0.20588673946142838 0.32254184013466236 >10 3.466676273264506 16.22933091411744 >50 2.366437593647358 30.087885477524196 >100 1.3395900326739552 32.06118427647465 >500 0.003467232464263292 0.4016485413739823 >1k 0.0012382973086654616 0.3716445288381562 >5k 2.476594617330923E-4 0.24067873848781185 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5583 0.23545000767542565 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 4106 0.17316097644909506 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.020327226168646816 0.0 2 0.0 0.0 0.0 0.0883095676289345 0.0 3 0.0 0.0 0.0 0.1389589423769528 0.0 4 0.0 0.0 0.0 0.28437030301905697 0.0 5 0.0 0.0 0.0 0.49371542895507936 0.0 6 0.0 0.0 0.0 0.7632409526974482 0.0 7 0.0 0.0 0.0 0.8786253734389787 0.0 8 0.0 0.0 0.0 1.1656525545672156 0.0 9 0.0 0.0 0.0 1.2486061933093906 0.0 10 0.0 0.0 0.0 1.4126578733841542 0.0 11 0.0 0.0 0.0 1.6793578283437443 0.0 12 0.0 0.0 0.0 1.8947758185293209 0.0 13 0.0 0.0 0.0 1.9864170269618304 0.0 14 0.0 0.0 0.0 2.025764126578734 0.0 15 0.0 0.0 0.0 2.084299790317493 0.0 16 0.0 0.0 0.0 2.209932169480146 0.0 17 0.0 0.0 0.0 2.353530105381064 0.0 18 0.0 0.0 0.0 2.526986290508957 0.0 19 0.0 0.0 0.0 2.6292128387097864 0.0 20 0.0 0.0 0.0 2.7429103527153296 0.0 21 0.0 0.0 0.0 2.8942680596017887 0.0 22 0.0 0.0 0.0 3.065995165325295 0.0 23 0.0 0.0 0.0 3.24042132182638 0.0 24 0.0 0.0 0.0 3.372970018606581 0.0 25 0.0 0.0 0.0 3.492403015514481 0.0 26 0.0 0.0 0.0 3.608673062292405 0.0 27 0.0 0.0 0.0 3.7193763168415708 0.0 28 0.0 0.0 0.0 3.8416348825322495 0.0 29 0.0 0.0 0.0 3.975870486048438 0.0 30 0.0 0.0 0.0 4.13473492791004 0.0 31 0.0 0.0 0.0 4.289213412258077 0.0 32 0.0 0.0 0.0 4.430280988055014 0.0 33 0.0 0.0 0.0 4.576493629396712 0.0 34 0.0 0.0 0.0 4.7242666594691975 0.0 35 0.0 0.0 0.0 4.908350357033811 0.0 36 0.0 0.0 0.0 5.08766854306926 0.0 37 0.0 0.0 0.0 5.2679567004779 0.0 38 0.0 0.0 0.0 5.443015447004981 0.0 39 0.0 0.0 0.0 5.6407209164626915 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTAC 20 7.0348056E-4 45.0 30 TACGACG 30 2.1664819E-6 44.999996 1 CGTAAGG 555 0.0 42.972973 2 GTATGCG 100 0.0 42.75 1 CGACGGT 500 0.0 42.3 28 CGGTCTA 510 0.0 41.47059 31 CAATCGA 50 1.0822987E-9 40.5 15 GGCGATA 1030 0.0 39.757282 8 TGCGTAG 210 0.0 39.642857 1 TACGGGA 990 0.0 39.31818 4 TTGGGAC 1660 0.0 39.171684 5 CGGGATA 505 0.0 38.762375 6 ACGGGAT 1135 0.0 38.656387 5 TCGTTAG 70 0.0 38.571426 1 TCGAATT 35 6.251099E-6 38.571426 11 CACGACG 550 0.0 38.454544 26 GCGATAT 205 0.0 38.414635 9 TAGGGTA 880 0.0 38.352272 5 TTAGGGA 3640 0.0 38.262363 4 AGGGCGA 2000 0.0 38.137497 6 >>END_MODULE