FastQCFastQC Report
Thu 26 May 2016
SRR1548033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548033_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2351241
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127350.5416288674789186No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA253.892056E-544.99999638
CGTTTTT81650.040.9491771
TAGGGAC31600.039.3755
GGGCGAT38550.038.9883277
TACGGGA10650.038.873244
GACCGAT13000.038.5961539
AGGGCGA20900.038.540676
ACGGGAT14200.038.503525
AAGGGAT50900.038.192535
CTAACGG1650.038.181822
TATGGGA34800.038.017244
GTAGGGA43100.038.004644
ATAGGGA43600.037.9300464
CGTAAGG3500.037.928572
TAAGGGA41000.037.9207344
TACGGGT1500.037.54
TACGATC301.1402852E-437.49999611
TGGGCGA9850.037.4619266
GGCGATA8950.037.45818
GGGACCG19500.037.3846137
Produced by FastQC (version 0.11.5)