##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548033_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2351241
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88236552526942	31.0	31.0	31.0	30.0	33.0
2	31.21937904281186	31.0	31.0	33.0	30.0	34.0
3	31.33800788604826	31.0	31.0	34.0	30.0	34.0
4	35.24848239716813	37.0	35.0	37.0	33.0	37.0
5	35.09568904250989	35.0	35.0	37.0	32.0	37.0
6	35.140481558462106	37.0	35.0	37.0	32.0	37.0
7	35.55744349473321	36.0	35.0	37.0	35.0	37.0
8	35.65001205746242	37.0	35.0	37.0	35.0	37.0
9	37.4616970357356	39.0	37.0	39.0	35.0	39.0
10	36.59762695529722	39.0	35.0	39.0	32.0	39.0
11	36.22277980011407	38.0	35.0	39.0	31.0	39.0
12	35.859333432855244	37.0	35.0	39.0	31.0	39.0
13	35.685373383672705	37.0	35.0	39.0	30.0	39.0
14	36.674946549503005	38.0	35.0	40.0	31.0	41.0
15	36.82892183319362	39.0	35.0	40.0	31.0	41.0
16	36.91389143010011	39.0	35.0	40.0	31.0	41.0
17	36.844636513228544	38.0	35.0	40.0	31.0	41.0
18	36.74210470130455	38.0	35.0	40.0	31.0	41.0
19	36.679643643505706	38.0	35.0	40.0	31.0	41.0
20	36.542911594345284	38.0	35.0	40.0	30.0	41.0
21	36.42875060446802	38.0	35.0	40.0	30.0	41.0
22	36.326323843451185	38.0	35.0	40.0	30.0	41.0
23	36.25178533378756	38.0	34.0	40.0	30.0	41.0
24	36.15004757062334	38.0	34.0	40.0	30.0	41.0
25	35.9711314152824	38.0	34.0	40.0	29.0	41.0
26	35.7563346334978	38.0	34.0	40.0	29.0	41.0
27	35.84711435365409	38.0	34.0	40.0	29.0	41.0
28	35.85572682681188	38.0	34.0	40.0	29.0	41.0
29	35.83760703390252	38.0	34.0	40.0	29.0	41.0
30	35.74673544736588	38.0	34.0	40.0	29.0	41.0
31	35.64375748806694	38.0	34.0	40.0	29.0	41.0
32	35.50966319488304	38.0	34.0	40.0	27.0	41.0
33	35.35077688760956	38.0	34.0	40.0	27.0	41.0
34	35.15785195987991	38.0	34.0	40.0	26.0	41.0
35	34.977503794804534	38.0	34.0	40.0	25.0	41.0
36	34.886200946649026	38.0	33.0	40.0	24.0	41.0
37	34.84823886619874	38.0	33.0	40.0	24.0	41.0
38	34.76614689859525	38.0	33.0	40.0	24.0	41.0
39	34.724855512471926	38.0	33.0	40.0	24.0	41.0
40	34.54514062999071	38.0	33.0	40.0	23.0	41.0
41	34.43114593527418	37.0	33.0	40.0	23.0	41.0
42	34.3937945961303	37.0	33.0	40.0	23.0	41.0
43	34.3754876680017	37.0	33.0	40.0	23.0	41.0
44	34.230591419595015	37.0	33.0	40.0	23.0	41.0
45	34.10764655771144	37.0	33.0	40.0	23.0	41.0
46	34.05011736355397	37.0	33.0	40.0	23.0	41.0
47	33.982969844435345	37.0	33.0	40.0	23.0	41.0
48	33.879632500454015	37.0	33.0	40.0	23.0	41.0
49	33.73677985370279	36.0	32.0	40.0	22.0	41.0
50	33.631777006270305	36.0	32.0	40.0	22.0	41.0
51	33.31716995407957	36.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	3.0
13	3.0
14	29.0
15	99.0
16	263.0
17	650.0
18	1385.0
19	2660.0
20	4496.0
21	7268.0
22	11067.0
23	15823.0
24	23029.0
25	32154.0
26	42031.0
27	48587.0
28	54609.0
29	63525.0
30	74458.0
31	89423.0
32	108951.0
33	134141.0
34	187651.0
35	213095.0
36	206521.0
37	255458.0
38	350223.0
39	423599.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.66697926754425	24.930451621080103	29.129680879161263	14.272888232214392
2	31.154611543436	26.452754098792937	29.365386193929076	13.027248163841987
3	28.08410537243949	26.452116137818283	32.19874100528189	13.265037484460334
4	25.2393097942746	28.9766127759766	31.168391500488468	14.615685929260335
5	22.940396156752964	32.99176902750504	30.30293364227657	13.764901173465416
6	22.20499727590664	41.79486492452284	26.12773424757394	9.872403551996584
7	90.93372393557274	2.5148421620752615	5.554768737020152	0.9966651653318396
8	91.38795214952444	2.079497592973243	5.314214918844985	1.2183353386573303
9	85.99041952739	5.493609544916919	6.603448987151891	1.9125219405411866
10	36.444328760854376	42.70961590070946	11.918046682581666	8.928008655854503
11	29.816892441055597	25.226337921123353	27.988411226241798	16.968358411579246
12	30.273119599394533	23.06067306584055	28.165934500121427	18.500272834643493
13	25.31152697660512	26.319675439480683	29.12997859428276	19.238818989631433
14	19.740724153755398	29.769343083078255	30.27966933206762	20.210263431098728
15	19.375087453816942	27.598744662924812	35.10091054043375	17.92525734282449
16	23.63543337326969	26.538155807932917	33.27515129244514	16.551259526352254
17	23.251423397261277	25.553654431851097	29.12742675038416	22.06749542050347
18	23.689702586846693	26.543854925973136	30.47616131226021	19.29028117491997
19	25.82053477291354	27.818543484057994	27.491099381135324	18.869822361893146
20	27.714173068605046	27.9344397277863	27.284570148274888	17.066817055333757
21	25.797270462704592	26.85917777037743	29.766110747473356	17.577441019444624
22	24.54758997482606	24.490173487107448	30.19975408730964	20.762482450756856
23	22.212695338334097	27.73526831150018	29.933299053563626	20.11873729660209
24	22.133630708208983	26.604758933686508	31.80477883806892	19.45683152003559
25	22.044188579562878	28.232027257095293	29.293041419403625	20.4307427439382
26	21.166481870637675	31.047306507499655	27.586665935138083	20.199545686724584
27	20.461492462916393	30.12668629034625	29.808386294726912	19.60343495201045
28	19.496937999975334	29.364833294417714	31.557164918440943	19.581063787166013
29	20.86302510036189	27.667346733065646	31.16830643902518	20.301321727547283
30	22.404253753656047	27.39170506128466	30.351206022691844	19.852835162367448
31	23.783652973046998	27.428876920741	28.670604161802217	20.11686594440978
32	25.005560893162375	27.56361427858735	28.610253053600204	18.82057177465007
33	24.575234950394282	27.556086339086466	28.49010373670755	19.378574973811702
34	21.716319169323775	28.480364199161208	30.030651898295414	19.772664733219607
35	21.96316753578217	27.72514599736905	30.1576911937143	20.153995273134484
36	23.812616401296165	28.41567495633157	28.356429647152293	19.41527899521997
37	22.50024561497524	29.584079216039527	28.64853071207928	19.26714445690595
38	22.384221779051998	29.611298884291315	27.66015903941791	20.344320297238777
39	22.382180303933115	28.47602606453358	28.025923331551294	21.11587029998201
40	24.187099493416454	27.084803301745758	28.145945056249015	20.58215214858877
41	20.429041514672463	27.206228540587713	29.84296377955301	22.52176616518681
42	21.70360248056239	27.66743179452893	28.27357978191091	22.35538594299776
43	22.186198692520247	27.45966917045084	28.706500099309256	21.647632037719656
44	21.56716389345031	28.50686084497506	28.550497375641203	21.375477885933428
45	21.336945043064492	28.91064761119766	27.46519816556448	22.287209180173363
46	21.420517930743806	28.894485933173165	28.04391383103646	21.64108230504657
47	21.11140457315945	28.242915124396013	28.96512947843288	21.68055082401166
48	21.456583991177425	27.407781677845872	30.08020020065999	21.055434130316712
49	21.945347159223576	26.735668525684947	29.670714316397174	21.648269998694307
50	20.81568839604277	28.662310669131745	29.066650334865717	21.455350599959765
51	20.20766905646848	28.804831150868836	28.528083680065127	22.45941611259756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1589.0
1	2040.0
2	2491.0
3	12599.0
4	22707.0
5	17480.5
6	12254.0
7	11379.0
8	10504.0
9	10850.5
10	11197.0
11	11569.5
12	11942.0
13	11961.0
14	11980.0
15	11541.0
16	11102.0
17	11086.5
18	11071.0
19	11190.0
20	11309.0
21	12551.0
22	13793.0
23	14735.0
24	15677.0
25	19240.0
26	24989.5
27	27176.0
28	33435.0
29	39694.0
30	46200.5
31	52707.0
32	59848.0
33	66989.0
34	75841.5
35	84694.0
36	90955.0
37	97216.0
38	105227.0
39	113238.0
40	125050.5
41	136863.0
42	146181.5
43	155500.0
44	164517.0
45	173534.0
46	172704.0
47	171874.0
48	174387.5
49	176901.0
50	170266.0
51	163631.0
52	152157.5
53	140684.0
54	131417.0
55	122150.0
56	115039.5
57	107929.0
58	102739.0
59	97549.0
60	91130.5
61	84712.0
62	73060.5
63	61409.0
64	51206.5
65	41004.0
66	33403.5
67	25803.0
68	20350.0
69	14897.0
70	12324.5
71	9752.0
72	7923.5
73	6095.0
74	5097.0
75	3164.5
76	2230.0
77	1771.5
78	1313.0
79	968.0
80	623.0
81	478.5
82	334.0
83	225.5
84	117.0
85	85.5
86	54.0
87	37.5
88	21.0
89	23.5
90	26.0
91	14.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2351241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.7831283705384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23247533233776	17.892636184623523
2	6.49987297729898	3.091746268225905
3	2.579533167557136	1.8404810538021867
4	1.5569887711646664	1.481202552643844
5	1.0853054579386516	1.2905979513700456
6	0.8820587784418722	1.2586870294826
7	0.7295938818898683	1.2146417465942316
8	0.653002513431908	1.2424354082588234
9	0.5705694184533079	1.2212933151040615
>10	9.151741503270959	51.93679673178235
>50	1.0108451188833145	14.913813174016177
>100	0.045997364390074805	1.7032395013627137
>500	0.001099480877197361	0.16016320591543914
>1k	7.329872514649073E-4	0.19725133876823878
>5k	0.0	0.0
>10k+	1.8324681286622683E-4	0.5550145380498279
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12735	0.5416288674789186	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7012292657366896E-4	0.0	0.0	0.026709299472066027	0.0
2	1.7012292657366896E-4	0.0	0.0	0.09076058132705239	0.0
3	1.7012292657366896E-4	0.0	0.0	0.14218023588394385	0.0
4	1.7012292657366896E-4	0.0	0.0	0.20895348456410892	0.0
5	1.7012292657366896E-4	0.0	0.0	0.3718461867583969	0.0
6	1.7012292657366896E-4	0.0	0.0	0.6769191248366289	0.0
7	1.7012292657366896E-4	0.0	0.0	0.8422786094662351	0.0
8	1.7012292657366896E-4	0.0	0.0	1.1649167397131983	0.0
9	1.7012292657366896E-4	0.0	0.0	1.3768048447607029	0.0
10	1.7012292657366896E-4	0.0	0.0	1.672818736998887	0.0
11	1.7012292657366896E-4	0.0	0.0	1.9967753199267961	0.0
12	1.7012292657366896E-4	0.0	0.0	2.240093635658786	0.0
13	1.7012292657366896E-4	0.0	0.0	2.390652425676483	0.0
14	1.7012292657366896E-4	0.0	0.0	2.499275914293771	0.0
15	1.7012292657366896E-4	0.0	0.0	2.5843799083122487	0.0
16	1.7012292657366896E-4	0.0	0.0	2.72030812664461	0.0
17	1.7012292657366896E-4	0.0	0.0	2.8758855429962304	0.0
18	1.7012292657366896E-4	0.0	0.0	3.0473694529824886	0.0
19	1.7012292657366896E-4	0.0	0.0	3.1784066371758573	0.0
20	1.7012292657366896E-4	0.0	0.0	3.3105496203919547	0.0
21	1.7012292657366896E-4	0.0	0.0	3.4710606016142114	0.0
22	1.7012292657366896E-4	0.0	0.0	3.6537300940226882	0.0
23	1.7012292657366896E-4	0.0	0.0	3.8541349015264705	0.0
24	1.7012292657366896E-4	0.0	0.0	4.005799490566896	0.0
25	1.7012292657366896E-4	0.0	0.0	4.1615470298450905	0.0
26	1.7012292657366896E-4	0.0	0.0	4.298708639395111	0.0
27	1.7012292657366896E-4	0.0	0.0	4.457858637204779	0.0
28	1.7012292657366896E-4	0.0	0.0	4.613521115019686	0.0
29	1.7012292657366896E-4	0.0	0.0	4.786791315734967	0.0
30	2.126536582170862E-4	0.0	0.0	5.006675198331434	0.0
31	2.126536582170862E-4	0.0	0.0	5.199722189260905	0.0
32	2.126536582170862E-4	0.0	0.0	5.394768124577617	0.0
33	2.126536582170862E-4	0.0	0.0	5.56905906285234	0.0
34	2.126536582170862E-4	0.0	0.0	5.7548758294024305	0.0
35	2.126536582170862E-4	0.0	0.0	5.956981866171949	0.0
36	2.126536582170862E-4	0.0	0.0	6.1522404551468775	0.0
37	2.126536582170862E-4	0.0	0.0	6.363703252877948	0.0
38	2.126536582170862E-4	0.0	0.0	6.598898198866046	0.0
39	2.126536582170862E-4	0.0	0.0	6.946586930050982	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTA	25	3.892056E-5	44.999996	38
CGTTTTT	8165	0.0	40.949177	1
TAGGGAC	3160	0.0	39.375	5
GGGCGAT	3855	0.0	38.988327	7
TACGGGA	1065	0.0	38.87324	4
GACCGAT	1300	0.0	38.596153	9
AGGGCGA	2090	0.0	38.54067	6
ACGGGAT	1420	0.0	38.50352	5
AAGGGAT	5090	0.0	38.19253	5
CTAACGG	165	0.0	38.18182	2
TATGGGA	3480	0.0	38.01724	4
GTAGGGA	4310	0.0	38.00464	4
ATAGGGA	4360	0.0	37.930046	4
CGTAAGG	350	0.0	37.92857	2
TAAGGGA	4100	0.0	37.920734	4
TACGGGT	150	0.0	37.5	4
TACGATC	30	1.1402852E-4	37.499996	11
TGGGCGA	985	0.0	37.461926	6
GGCGATA	895	0.0	37.4581	8
GGGACCG	1950	0.0	37.384613	7
>>END_MODULE