FastQCFastQC Report
Thu 26 May 2016
SRR1548032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548032_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2460557
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201470.8187983452527213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT302.1665164E-645.00000427
CGTTTTT110750.042.5823941
CGACGGT1050.040.71428728
TAGTACG1450.040.3448261
GACCGAT13900.039.9820149
TAGGGAT54800.039.3339425
AGGGCGA20350.039.140056
CACGACC2550.038.8235327
TAGGGAC29350.038.7904625
GCGATAT2150.038.7209329
GTAGGGA40350.038.5873574
CGGCGAA356.2511954E-638.57142631
CGCATCG356.2511954E-638.57142621
GGGCGAT40450.038.436347
AGGGATT69050.038.091966
AAGGGAT58700.038.0621835
AGGGACC34200.038.0263186
TTTCGCG1600.037.968751
TAAGGGA43750.037.9028554
TAGCGCG1250.037.81
Produced by FastQC (version 0.11.5)