##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548032_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2460557
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.816435872040355	31.0	31.0	31.0	30.0	33.0
2	31.152910093121193	31.0	31.0	33.0	30.0	34.0
3	31.211280616543327	31.0	31.0	34.0	28.0	34.0
4	35.14002561208702	35.0	35.0	37.0	32.0	37.0
5	34.992974354993606	35.0	35.0	37.0	32.0	37.0
6	35.03759270766741	36.0	35.0	37.0	32.0	37.0
7	35.5289229227366	35.0	35.0	37.0	35.0	37.0
8	35.6372996033012	37.0	35.0	37.0	35.0	37.0
9	37.4422693723413	39.0	37.0	39.0	35.0	39.0
10	36.52567325203196	38.0	35.0	39.0	32.0	39.0
11	36.08432318373441	38.0	35.0	39.0	31.0	39.0
12	35.769559087637475	37.0	35.0	39.0	30.0	39.0
13	35.58753932544542	37.0	35.0	39.0	30.0	39.0
14	36.56958851186947	38.0	35.0	40.0	30.0	41.0
15	36.762824027242615	38.0	35.0	40.0	31.0	41.0
16	36.85584158383651	38.0	35.0	40.0	31.0	41.0
17	36.77883137842367	38.0	35.0	40.0	31.0	41.0
18	36.669329342908945	38.0	35.0	40.0	31.0	41.0
19	36.60431195050552	38.0	35.0	40.0	31.0	41.0
20	36.44389176922136	38.0	35.0	40.0	30.0	41.0
21	36.32976557746884	38.0	34.0	40.0	30.0	41.0
22	36.22031068575124	38.0	34.0	40.0	30.0	41.0
23	36.15061020736362	38.0	34.0	40.0	30.0	41.0
24	36.04157757775983	38.0	34.0	40.0	30.0	41.0
25	35.88706053141626	38.0	34.0	40.0	29.0	41.0
26	35.659093449166186	38.0	34.0	40.0	29.0	41.0
27	35.71968216952503	38.0	34.0	40.0	29.0	41.0
28	35.735550527787	38.0	34.0	40.0	29.0	41.0
29	35.73315960573155	38.0	34.0	40.0	29.0	41.0
30	35.64450244395883	38.0	34.0	40.0	29.0	41.0
31	35.525460698532896	38.0	34.0	40.0	28.0	41.0
32	35.363998883179704	38.0	34.0	40.0	27.0	41.0
33	35.17411911205471	38.0	34.0	40.0	26.0	41.0
34	34.97143126535983	38.0	34.0	40.0	25.0	41.0
35	34.778427811263875	38.0	33.0	40.0	24.0	41.0
36	34.68533913256226	38.0	33.0	40.0	24.0	41.0
37	34.623750638574926	38.0	33.0	40.0	24.0	41.0
38	34.557397369782535	38.0	33.0	40.0	24.0	41.0
39	34.512484774788796	37.0	33.0	40.0	23.0	41.0
40	34.35047430317607	37.0	33.0	40.0	23.0	41.0
41	34.26057473978453	37.0	33.0	40.0	23.0	41.0
42	34.2156962834025	37.0	33.0	40.0	23.0	41.0
43	34.17532981353409	37.0	33.0	40.0	23.0	41.0
44	34.031159205009274	37.0	33.0	40.0	23.0	41.0
45	33.92691979905363	37.0	33.0	40.0	23.0	41.0
46	33.87579194466944	37.0	33.0	40.0	22.0	41.0
47	33.825472850253014	37.0	33.0	40.0	22.0	41.0
48	33.72762508651496	36.0	32.0	40.0	22.0	41.0
49	33.60391895005887	36.0	32.0	40.0	21.0	41.0
50	33.497773471616384	36.0	32.0	40.0	20.0	41.0
51	33.17955731161684	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	14.0
14	36.0
15	98.0
16	295.0
17	720.0
18	1583.0
19	2915.0
20	5140.0
21	8054.0
22	12137.0
23	17894.0
24	26015.0
25	36725.0
26	47605.0
27	54780.0
28	60137.0
29	68010.0
30	79949.0
31	94794.0
32	114852.0
33	141584.0
34	196853.0
35	230511.0
36	213655.0
37	259118.0
38	351366.0
39	435667.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.677049139686666	26.787349368456002	30.872521953362593	15.66307953849474
2	30.970223408764763	27.052126815188593	28.621974617942197	13.355675158104447
3	26.92272522034645	26.355495930392998	33.01992191198985	13.701856937270707
4	24.427680399194166	28.46444930964818	32.30077579995098	14.807094491206666
5	22.348679587589316	32.912425926324815	31.01752977069826	13.721364715387613
6	20.990531818608552	41.136417485959484	27.743718190637324	10.129332504794645
7	89.90057942165127	2.4420893318057657	6.656419664328037	1.0009115822149213
8	90.84642217189035	1.7816291189352653	6.233222802804406	1.1387259063699804
9	85.86405435842373	5.230075954346923	7.324642347240889	1.5812273399884662
10	36.78772733165702	42.51716176459232	12.1793154964506	8.515795407300054
11	26.302012105389146	26.048614195891417	31.53899706448581	16.11037663423363
12	27.81191413163767	24.016757181402422	30.45851000403567	17.712818682924233
13	22.785938305838883	28.612058164066106	31.34416313054321	17.257840399551807
14	17.792678649590314	32.05875742768812	31.344122489338794	18.804441433382767
15	16.845413457196887	28.796853720519376	37.833141032701135	16.5245917895826
16	20.996424793248032	26.998968119820027	36.0910151644526	15.913591922479341
17	20.781432821917964	26.652014157769973	30.68459702416973	21.881955996142338
18	21.07518744739504	27.78041719821975	32.8523175850021	18.292077769383113
19	23.572914588038397	28.716302853378323	29.547212277545288	18.163570281037995
20	25.099195019664243	29.145636536767896	29.061631167251967	16.693537276315894
21	23.378649630957543	28.006869989193504	31.59000177602063	17.02447860382832
22	22.22261057150881	25.800052589718508	31.73732614200768	20.240010696765
23	19.4320229118854	29.529167582787146	31.23808958703253	19.800719918294924
24	19.212682331683435	28.52780894732372	34.673368672215275	17.586140048777573
25	19.97551773846328	29.6005335377315	31.72326428528175	18.700684438523474
26	19.129489786255714	32.91588042869968	28.84342854077349	19.111201244271115
27	18.459804019984094	32.20002625421805	31.477750769439606	17.862418956358255
28	17.353144023893776	30.94945575331114	33.58268066945818	18.114719553336908
29	18.43903636453047	29.130070955478782	33.04971191482254	19.381180765168214
30	20.106626263890657	29.74562263747599	31.653198848878528	18.494552249754832
31	21.918004744454205	29.588828870861356	29.679458756696146	18.813707627988297
32	23.049943569687674	29.29966670148263	29.89802715401432	17.75236257481538
33	21.936902904504958	29.38204642282215	29.520876777087462	19.16017389558543
34	19.143795490208113	29.92265572388691	31.01403462711898	19.919514158785997
35	18.868532612737685	29.47775645920822	31.305472703944677	20.34823822410942
36	21.652820885677514	29.881811313454637	29.295196168997506	19.170171631870346
37	19.188947868307867	32.09456232877352	29.98142290546409	18.73506689745452
38	19.396096087186763	31.899281341582412	28.6207960230143	20.08382654821652
39	18.967412663067755	31.795077293474606	29.071222491492783	20.16628755196486
40	21.33850181076886	29.2685355389044	29.384078483042657	20.008884167284073
41	18.392258338254305	29.24805237188165	30.31464014042349	22.045049149440555
42	19.976899539413232	30.08704939572625	28.52472021578854	21.411330849071977
43	19.83693123142443	30.03827995043399	29.074961482298523	21.049827335843062
44	19.612916912715292	31.21220113982322	28.94649463515781	20.228387312303678
45	18.91795231730051	31.829419111201247	27.95241077528381	21.300217796214437
46	19.955481624689046	30.892151655092732	28.986160450662187	20.16620626955604
47	19.823235145538185	30.1349653757259	29.638614346263875	20.40318513247204
48	19.571097113377174	29.66149534434683	30.55214733899682	20.215260203279175
49	19.84814820384165	28.78474264160513	30.4113255657154	20.95578358883781
50	18.776399002339712	31.319046866217693	29.501612846197016	20.402941285245575
51	18.168731713998092	31.465030072459204	28.932432778431878	21.43380543511083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1995.0
1	2355.0
2	2715.0
3	17419.5
4	32124.0
5	24014.0
6	15904.0
7	15187.5
8	14471.0
9	15543.0
10	16615.0
11	17934.0
12	19253.0
13	19777.5
14	20302.0
15	20016.5
16	19731.0
17	18946.5
18	18162.0
19	18046.0
20	17930.0
21	18407.5
22	18885.0
23	21108.5
24	23332.0
25	26251.0
26	34272.0
27	39374.0
28	47323.0
29	55272.0
30	61172.5
31	67073.0
32	77112.0
33	87151.0
34	93469.5
35	99788.0
36	106692.5
37	113597.0
38	122243.5
39	130890.0
40	147750.5
41	164611.0
42	173680.0
43	182749.0
44	186072.5
45	189396.0
46	192870.5
47	196345.0
48	189916.5
49	183488.0
50	173755.0
51	164022.0
52	148699.5
53	133377.0
54	118731.0
55	104085.0
56	94535.5
57	84986.0
58	77198.5
59	69411.0
60	61768.5
61	54126.0
62	45704.5
63	37283.0
64	29875.0
65	22467.0
66	17549.5
67	12632.0
68	10019.0
69	7406.0
70	5805.5
71	4205.0
72	3287.0
73	2369.0
74	2046.0
75	1419.0
76	1115.0
77	814.0
78	513.0
79	366.5
80	220.0
81	174.5
82	129.0
83	105.5
84	82.0
85	62.5
86	43.0
87	32.0
88	21.0
89	18.0
90	15.0
91	8.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2460557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.469164386973567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88566617141268	18.323896757763226
2	7.612887529755515	3.725619928502576
3	3.2497107722773415	2.3855312129091923
4	1.9260947130822235	1.8851971263715845
5	1.2676792042027587	1.550952541879257
6	0.9157153476937426	1.344407362463571
7	0.7368066743214938	1.2620330544774352
8	0.5695707782685969	1.1149536802776618
9	0.5102143855973764	1.123606770640283
>10	6.8757498337364025	40.815735613948675
>50	1.3473088631408001	21.621471813871302
>100	0.10021391664119315	3.538479398222326
>500	0.0017012927642230185	0.2645536442817905
>1k	5.103878292669054E-4	0.20485735381757528
>5k	0.0	0.0
>10k+	1.701292764223018E-4	0.8387037405736008
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20147	0.8187983452527213	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.064120441022094E-5	0.0	0.0	0.009672606649632582	0.0
2	4.064120441022094E-5	0.0	0.0	0.058279487124256825	0.0
3	4.064120441022094E-5	0.0	0.0	0.12428080308645563	0.0
4	4.064120441022094E-5	0.0	0.0	0.2243800895488298	0.0
5	4.064120441022094E-5	0.0	0.0	0.4528242995386817	0.0
6	4.064120441022094E-5	0.0	0.0	0.8604962209776079	0.0
7	4.064120441022094E-5	0.0	0.0	1.0945895583804806	0.0
8	4.064120441022094E-5	0.0	0.0	1.4985224890136664	0.0
9	4.064120441022094E-5	0.0	0.0	1.7285110647711066	0.0
10	4.064120441022094E-5	0.0	0.0	2.0049525371694297	0.0
11	4.064120441022094E-5	0.0	0.0	2.3269934409160205	0.0
12	4.064120441022094E-5	0.0	0.0	2.5786844198285186	0.0
13	4.064120441022094E-5	0.0	0.0	2.737347681846021	0.0
14	4.064120441022094E-5	0.0	0.0	2.8437463549919793	0.0
15	4.064120441022094E-5	0.0	0.0	2.9344168820311824	0.0
16	4.064120441022094E-5	0.0	0.0	3.064387453735069	0.0
17	4.064120441022094E-5	0.0	0.0	3.232235627949281	0.0
18	4.064120441022094E-5	0.0	0.0	3.4191038858274774	0.0
19	4.064120441022094E-5	0.0	0.0	3.557243339617818	0.0
20	4.064120441022094E-5	0.0	0.0	3.696317541109594	0.0
21	4.064120441022094E-5	0.0	0.0	3.870139972372109	0.0
22	4.064120441022094E-5	0.0	0.0	4.073996253693777	0.0
23	4.064120441022094E-5	0.0	0.0	4.284314486516671	0.0
24	4.064120441022094E-5	0.0	0.0	4.458583971027698	0.0
25	4.064120441022094E-5	0.0	0.0	4.614077219101204	0.0
26	4.064120441022094E-5	0.0	0.0	4.756727846581079	0.0
27	4.064120441022094E-5	0.0	0.0	4.92051190035427	0.0
28	4.064120441022094E-5	0.0	0.0	5.085393266646536	0.0
29	4.064120441022094E-5	0.0	0.0	5.2689289457630935	0.0
30	4.064120441022094E-5	0.0	0.0	5.492577493632539	0.0
31	4.064120441022094E-5	0.0	0.0	5.705943816786199	0.0
32	4.064120441022094E-5	0.0	0.0	5.9008590331376185	0.0
33	4.064120441022094E-5	0.0	0.0	6.0936202656552965	0.0
34	4.064120441022094E-5	0.0	0.0	6.299589889606296	0.0
35	4.064120441022094E-5	0.0	0.0	6.524417032403639	0.0
36	4.064120441022094E-5	0.0	0.0	6.7348165476353525	0.0
37	4.064120441022094E-5	0.0	0.0	6.957652271416594	0.0
38	4.064120441022094E-5	0.0	0.0	7.211903646206936	0.0
39	4.064120441022094E-5	0.0	0.0	7.580885141047332	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGAT	30	2.1665164E-6	45.000004	27
CGTTTTT	11075	0.0	42.582394	1
CGACGGT	105	0.0	40.714287	28
TAGTACG	145	0.0	40.344826	1
GACCGAT	1390	0.0	39.982014	9
TAGGGAT	5480	0.0	39.333942	5
AGGGCGA	2035	0.0	39.14005	6
CACGACC	255	0.0	38.82353	27
TAGGGAC	2935	0.0	38.790462	5
GCGATAT	215	0.0	38.720932	9
GTAGGGA	4035	0.0	38.587357	4
CGGCGAA	35	6.2511954E-6	38.571426	31
CGCATCG	35	6.2511954E-6	38.571426	21
GGGCGAT	4045	0.0	38.43634	7
AGGGATT	6905	0.0	38.09196	6
AAGGGAT	5870	0.0	38.062183	5
AGGGACC	3420	0.0	38.026318	6
TTTCGCG	160	0.0	37.96875	1
TAAGGGA	4375	0.0	37.902855	4
TAGCGCG	125	0.0	37.8	1
>>END_MODULE