FastQCFastQC Report
Thu 26 May 2016
SRR1548031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548031_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1152115
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34020.2952830229621175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22120.19199472274903112No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG600.045.0000041
TTGATCG207.033295E-445.020
CGAAACG207.033295E-445.036
ACCGAGC253.8908118E-545.033
GCGCATC207.033295E-445.019
TCTAGCG207.033295E-445.01
ACTAGTC253.8908118E-545.022
TGCGCAT253.8908118E-545.022
AGTTGCG502.1827873E-1145.01
TATTACG406.8157533E-945.01
ACTCGAT207.033295E-445.020
TATAGCG253.8908118E-545.01
TTCGCGT351.211938E-745.041
GCGTAAG1000.045.01
CACGACG3850.044.41558526
TCTCACG3800.044.40789423
TCACGAC3800.043.8157925
CGGTCTA3800.043.8157931
CGACGGT3800.043.22368228
CGTAAGG2200.042.9545442
Produced by FastQC (version 0.11.5)