##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548031_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1152115
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.006881257513356	31.0	31.0	33.0	30.0	33.0
2	31.356116359911987	31.0	31.0	33.0	30.0	34.0
3	31.538151139426187	31.0	31.0	34.0	30.0	34.0
4	35.40065271261983	37.0	35.0	37.0	33.0	37.0
5	35.24718712975701	37.0	35.0	37.0	33.0	37.0
6	35.27443267382162	37.0	35.0	37.0	32.0	37.0
7	35.60130976508422	36.0	35.0	37.0	35.0	37.0
8	35.69418851416742	37.0	35.0	37.0	35.0	37.0
9	37.556052998181606	39.0	37.0	39.0	35.0	39.0
10	36.76057511619934	39.0	37.0	39.0	32.0	39.0
11	36.39828662937293	38.0	35.0	39.0	32.0	39.0
12	35.49625080829605	37.0	35.0	39.0	30.0	39.0
13	34.98698827807988	37.0	34.0	39.0	28.0	39.0
14	35.95861958224656	38.0	34.0	40.0	29.0	41.0
15	36.32581643325536	38.0	35.0	40.0	30.0	41.0
16	36.55361400554632	38.0	35.0	40.0	31.0	41.0
17	36.51044991168416	38.0	35.0	40.0	31.0	41.0
18	36.42327198239759	38.0	35.0	40.0	31.0	41.0
19	36.35670397486362	38.0	35.0	40.0	31.0	41.0
20	36.23278578961302	38.0	34.0	40.0	31.0	41.0
21	36.068750949340995	37.0	34.0	40.0	30.0	41.0
22	35.946622515981474	37.0	34.0	40.0	30.0	41.0
23	35.925714012924054	37.0	34.0	40.0	30.0	41.0
24	35.80220290509194	37.0	34.0	40.0	30.0	41.0
25	35.65841343963059	37.0	34.0	40.0	30.0	41.0
26	35.43436462505913	36.0	34.0	40.0	29.0	41.0
27	35.49709621001376	37.0	34.0	40.0	29.0	41.0
28	35.55742438905838	37.0	34.0	40.0	29.0	41.0
29	35.59605421333808	37.0	34.0	40.0	30.0	41.0
30	35.543259136457735	36.0	34.0	40.0	29.0	41.0
31	35.388564509619265	36.0	34.0	40.0	29.0	41.0
32	35.2427691680084	36.0	34.0	40.0	29.0	41.0
33	35.09516324325263	36.0	34.0	40.0	27.0	41.0
34	34.95619794898947	36.0	34.0	40.0	27.0	41.0
35	34.80253707312204	36.0	34.0	40.0	26.0	41.0
36	34.660513924391225	36.0	33.0	40.0	25.0	41.0
37	34.58999579035079	36.0	33.0	40.0	25.0	41.0
38	34.548399248338924	36.0	33.0	40.0	25.0	41.0
39	34.52674949983292	36.0	33.0	40.0	25.0	41.0
40	34.30045264578623	36.0	33.0	40.0	23.0	41.0
41	34.26846191569418	36.0	33.0	40.0	24.0	41.0
42	34.22783576292297	36.0	33.0	40.0	24.0	41.0
43	34.18249393506725	35.0	33.0	40.0	24.0	41.0
44	33.99891590683222	35.0	33.0	40.0	23.0	41.0
45	33.86975692530694	35.0	33.0	40.0	23.0	41.0
46	33.823508069940935	35.0	33.0	40.0	23.0	41.0
47	33.793873007468875	35.0	33.0	40.0	23.0	41.0
48	33.72658371777123	35.0	33.0	40.0	23.0	41.0
49	33.61760588135733	35.0	33.0	39.0	23.0	41.0
50	33.46708618497286	35.0	32.0	39.0	23.0	41.0
51	33.12164063483246	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	7.0
14	13.0
15	43.0
16	124.0
17	324.0
18	652.0
19	1298.0
20	2112.0
21	3399.0
22	5244.0
23	7554.0
24	10980.0
25	15391.0
26	18964.0
27	22015.0
28	25686.0
29	30695.0
30	37007.0
31	45007.0
32	56496.0
33	73916.0
34	120958.0
35	144913.0
36	89487.0
37	108200.0
38	148050.0
39	183544.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.585835615368257	26.070661348910484	30.09855786965711	13.24494516606415
2	28.110301489000665	27.915095281287023	30.479075439517757	13.495527790194556
3	28.79295903620732	27.059451530446175	31.381676308354635	12.765913124991862
4	25.75090160270459	29.458691189681584	30.456768638547366	14.333638569066457
5	23.732700294675443	33.06336607022736	29.603815591325517	13.600118043771673
6	21.14623974169245	43.480728920290076	25.26761651397647	10.105414824041002
7	90.94612950964095	3.565789873406735	4.496599731797607	0.9914808851546938
8	91.15982345512383	3.0193166480776656	4.005242532212496	1.8156173645860005
9	86.86398493205974	5.818950365197919	5.503617260429731	1.8134474423126163
10	44.11981442824718	36.21261766403527	10.281352121966991	9.386215785750554
11	42.12791257817145	22.23180845662108	21.328079228202046	14.312199737005422
12	39.63614743319894	22.351588166111892	22.67152150609965	15.340742894589516
13	23.46493188613984	37.07477118169627	23.85004969122006	15.610247240943828
14	16.920533106504124	39.85800028642974	26.56158456404092	16.659882043025217
15	15.21254388667798	26.51375947713553	42.4500158404326	15.823680795753898
16	18.886222295517374	23.297847871089257	40.468269226596306	17.347660606797064
17	18.94741410362681	23.55606862162197	26.379050702403838	31.11746657234738
18	21.670926947396744	25.857227794100417	32.539112848977744	19.93273240952509
19	27.184005068938433	27.201538040907376	25.11589554862145	20.498561341532746
20	29.046579551520463	26.70011240197376	25.407272711491473	18.8460353350143
21	22.869071229868545	29.45860439279065	28.433967095298645	19.238357282042156
22	24.010884330123293	24.885536600078986	26.855826024311806	24.24775304548591
23	19.70775486822062	30.659265785099578	26.053128376941537	23.579850969738263
24	20.291550756651898	26.335825850718024	35.67386936199946	17.698754030630624
25	19.042283105419163	27.30690946650291	32.345035000846266	21.305772427231656
26	18.59232802281022	35.10604410150028	25.51828593499781	20.783341940691685
27	18.37316587319842	35.24509272077874	27.867009803708832	18.514731602314004
28	15.388654778385838	31.25660198851677	35.3713821970897	17.98336103600769
29	17.088137902900318	26.340512882828538	34.96170087187477	21.609648342396376
30	18.93179066325844	31.664981360367673	30.59998350859072	18.803244467783166
31	26.674854506711572	28.312104260425393	25.898109129730972	19.114932103132066
32	27.86067363067055	27.340847050858642	26.743163659877705	18.05531565859311
33	26.250244116255754	28.56034336850054	25.204775564939265	19.98463695030444
34	19.87709560243552	28.772474969946575	27.871436445146536	23.478992982471368
35	19.59031867478507	27.781514866137496	29.903525255725338	22.724641203352096
36	28.55799985244529	25.426888808842868	27.077505283760733	18.93760605495111
37	20.344236469449665	31.761673096869668	28.609644002551825	19.28444643112884
38	20.499776498005843	33.2654292323249	24.326911810018963	21.907882459650295
39	20.09799368986603	31.358241147802087	27.927854424254523	20.61591073807736
40	24.155661544203486	26.95381971417784	25.6731315884265	23.217387153192174
41	18.38835532911211	25.110253750710648	28.851894125152437	27.649496795024803
42	21.147454898165545	26.63458074931756	26.082465726077693	26.1354986264392
43	21.75459915025844	25.97700750359122	27.118733806955035	25.14965953919531
44	19.768946676330053	29.621348563294465	28.72447628925932	21.885228471116164
45	18.681642023582715	34.171588773690125	24.67019351366834	22.476575689058816
46	22.394726220906765	30.93232880398224	26.372454138692753	20.300490836418238
47	21.404547289116106	27.63161663549212	27.73577290461456	23.228063170777222
48	21.967685517504762	25.902362177386806	31.389227637866014	20.740724667242418
49	21.65547710081025	24.39895322949532	31.15565720435894	22.78991246533549
50	20.397790151156787	30.499472708887566	28.57075899541278	20.53197814454286
51	18.623227715983212	32.00331564123373	25.940118824943692	23.433337817839366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1264.0
1	1354.5
2	1445.0
3	3843.0
4	6241.0
5	5020.5
6	3800.0
7	3944.5
8	4089.0
9	4523.5
10	4958.0
11	5205.0
12	5452.0
13	5428.5
14	5405.0
15	5340.5
16	5276.0
17	5325.5
18	5375.0
19	5395.5
20	5416.0
21	5899.5
22	6383.0
23	6936.0
24	7489.0
25	7815.5
26	10128.0
27	12114.0
28	14128.0
29	16142.0
30	18550.5
31	20959.0
32	25420.5
33	29882.0
34	33140.5
35	36399.0
36	37964.5
37	39530.0
38	45662.0
39	51794.0
40	64264.5
41	76735.0
42	90424.5
43	104114.0
44	107550.5
45	110987.0
46	106058.5
47	101130.0
48	97459.0
49	93788.0
50	87001.5
51	80215.0
52	74347.5
53	68480.0
54	60541.0
55	52602.0
56	47832.5
57	43063.0
58	40859.0
59	38655.0
60	34167.5
61	29680.0
62	27322.0
63	24964.0
64	21435.0
65	17906.0
66	15286.5
67	12667.0
68	10118.0
69	7569.0
70	6019.0
71	4469.0
72	3603.5
73	2738.0
74	2422.5
75	1796.5
76	1486.0
77	1024.5
78	563.0
79	469.5
80	376.0
81	252.0
82	128.0
83	88.0
84	48.0
85	54.5
86	61.0
87	38.5
88	16.0
89	13.0
90	10.0
91	5.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1152115.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.187513862362565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8387370198406	16.117456103415513
2	6.950312921517979	2.806190769018037
3	2.3060691302462284	1.3966140760323633
4	1.1374136904641396	0.9184621857394313
5	0.7248682546042589	0.7316643969105017
6	0.5151073148196357	0.6239241635115453
7	0.39148166435089793	0.5532129068161165
8	0.3045579534552316	0.4918614325816209
9	0.2749843156342331	0.4996124715418527
>10	4.549893654823952	23.94962173208358
>50	2.360837493594733	34.23475980412527
>100	0.6413557414199209	16.715294835292845
>500	0.0035046761826225186	0.4648320925599809
>1k	8.761690456556297E-4	0.4964930303713343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3402	0.2952830229621175	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2212	0.19199472274903112	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03654149108378938	0.0
2	0.0	0.0	0.0	0.15267573115531002	0.0
3	0.0	0.0	0.0	0.21725261801122284	0.0
4	0.0	0.0	0.0	0.4372827365323774	0.0
5	0.0	0.0	0.0	0.7013188787577629	0.0
6	0.0	0.0	0.0	1.1175099707928462	0.0
7	0.0	0.0	0.0	1.267755389001966	0.0
8	0.0	0.0	0.0	1.6530467878640587	0.0
9	0.0	0.0	0.0	1.7584182134595938	0.0
10	0.0	0.0	0.0	1.9746292687795923	0.0
11	0.0	0.0	0.0	2.362524574369746	0.0
12	0.0	0.0	0.0	2.6325497020696718	0.0
13	0.0	0.0	0.0	2.7562352716525695	0.0
14	0.0	0.0	0.0	2.805362311921987	0.0
15	0.0	0.0	0.0	2.874713027779345	0.0
16	0.0	0.0	0.0	3.0220073516966623	0.0
17	0.0	0.0	0.0	3.1907405076750153	0.0
18	0.0	0.0	0.0	3.386293902952396	0.0
19	0.0	0.0	0.0	3.5057264248794606	0.0
20	0.0	0.0	0.0	3.634272620354739	0.0
21	0.0	0.0	0.0	3.817327263337427	0.0
22	0.0	0.0	0.0	4.002812219266306	0.0
23	0.0	0.0	0.0	4.210690773056509	0.0
24	0.0	0.0	0.0	4.348958220316549	0.0
25	0.0	0.0	0.0	4.4749873059547	0.0
26	0.0	0.0	0.0	4.6001484226835	0.0
27	0.0	0.0	0.0	4.723920789157332	0.0
28	0.0	0.0	0.0	4.858629563889021	0.0
29	0.0	0.0	0.0	5.00609748158821	0.0
30	0.0	0.0	0.0	5.173441887311596	0.0
31	0.0	0.0	0.0	5.336186057815409	0.0
32	0.0	0.0	0.0	5.484087959969274	0.0
33	0.0	0.0	0.0	5.637631660033938	0.0
34	0.0	0.0	0.0	5.798379502046236	0.0
35	0.0	0.0	0.0	6.003306961544637	0.0
36	0.0	0.0	0.0	6.1728212895414085	0.0
37	0.0	0.0	0.0	6.353098432014165	0.0
38	0.0	0.0	0.0	6.538062606597432	0.0
39	0.0	0.0	0.0	6.790641559219349	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAACG	60	0.0	45.000004	1
TTGATCG	20	7.033295E-4	45.0	20
CGAAACG	20	7.033295E-4	45.0	36
ACCGAGC	25	3.8908118E-5	45.0	33
GCGCATC	20	7.033295E-4	45.0	19
TCTAGCG	20	7.033295E-4	45.0	1
ACTAGTC	25	3.8908118E-5	45.0	22
TGCGCAT	25	3.8908118E-5	45.0	22
AGTTGCG	50	2.1827873E-11	45.0	1
TATTACG	40	6.8157533E-9	45.0	1
ACTCGAT	20	7.033295E-4	45.0	20
TATAGCG	25	3.8908118E-5	45.0	1
TTCGCGT	35	1.211938E-7	45.0	41
GCGTAAG	100	0.0	45.0	1
CACGACG	385	0.0	44.415585	26
TCTCACG	380	0.0	44.407894	23
TCACGAC	380	0.0	43.81579	25
CGGTCTA	380	0.0	43.81579	31
CGACGGT	380	0.0	43.223682	28
CGTAAGG	220	0.0	42.954544	2
>>END_MODULE