FastQCFastQC Report
Thu 26 May 2016
SRR1548030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548030_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1799518
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.522973373981255No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20690.11497523225663761No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.0343504E-445.0000041
CGCATAA207.0343504E-445.00000432
TACGCAC302.166189E-645.00000430
GCGATAC800.045.0000049
CCGACGG207.0343504E-445.0000042
GGACCGT207.0343504E-445.0000048
ACGCCGA253.891688E-545.012
TAGCGCG253.891688E-545.01
CCAGTCG253.891688E-545.028
CCTTGCG253.891688E-545.014
TGGTACG502.1827873E-1145.01
CGTTTTT46600.042.3927041
CGGTCTA1700.042.3529431
ATCCGCG556.184564E-1140.9090921
TCTACGG1600.040.7812542
TACGGGA6300.040.357144
TGTAGCG950.040.2631571
TACGTAG950.040.2631571
GTACCGG451.929402E-840.0000042
CGGACGA451.929402E-840.00000431