##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548030_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1799518
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80018816149658	31.0	31.0	31.0	30.0	33.0
2	31.13495002550683	31.0	31.0	33.0	30.0	34.0
3	31.263365523434608	31.0	31.0	34.0	28.0	34.0
4	35.1501818820373	35.0	35.0	37.0	32.0	37.0
5	34.98239917577929	35.0	35.0	37.0	32.0	37.0
6	35.02092060207233	36.0	35.0	37.0	32.0	37.0
7	35.49723981643974	35.0	35.0	37.0	35.0	37.0
8	35.59766781993845	37.0	35.0	37.0	35.0	37.0
9	37.42523386818026	39.0	37.0	39.0	35.0	39.0
10	36.444085582917204	38.0	35.0	39.0	32.0	39.0
11	36.00374655880074	38.0	35.0	39.0	31.0	39.0
12	35.49778607382643	37.0	35.0	39.0	30.0	39.0
13	35.22572322144041	37.0	34.0	39.0	30.0	39.0
14	36.247873041558904	38.0	35.0	40.0	30.0	41.0
15	36.49311371156054	38.0	35.0	40.0	30.0	41.0
16	36.631889761591715	38.0	35.0	40.0	31.0	41.0
17	36.56532749325097	38.0	35.0	40.0	31.0	41.0
18	36.489448285596474	38.0	35.0	40.0	31.0	41.0
19	36.41771407676944	38.0	35.0	40.0	30.0	41.0
20	36.298709432192396	38.0	34.0	40.0	30.0	41.0
21	36.1888627954819	38.0	34.0	40.0	30.0	41.0
22	36.026629908675545	38.0	34.0	40.0	30.0	41.0
23	35.952450600660846	38.0	34.0	40.0	30.0	41.0
24	35.82655077637456	37.0	34.0	40.0	29.0	41.0
25	35.66575216252352	37.0	34.0	40.0	29.0	41.0
26	35.40477227790998	37.0	34.0	40.0	29.0	41.0
27	35.45717797765846	37.0	34.0	40.0	29.0	41.0
28	35.48383011450844	37.0	34.0	40.0	29.0	41.0
29	35.48850025395689	37.0	34.0	40.0	29.0	41.0
30	35.41407310179726	37.0	34.0	40.0	29.0	41.0
31	35.33333425950727	37.0	34.0	40.0	28.0	41.0
32	35.18603370458089	37.0	34.0	40.0	27.0	41.0
33	35.058611250345926	37.0	34.0	40.0	27.0	41.0
34	34.94734423328914	37.0	34.0	40.0	26.0	41.0
35	34.80890883003115	37.0	33.0	40.0	25.0	41.0
36	34.696767690014774	37.0	33.0	40.0	25.0	41.0
37	34.61460235463052	37.0	33.0	40.0	24.0	41.0
38	34.57901226884088	37.0	33.0	40.0	24.0	41.0
39	34.53509217468233	37.0	33.0	40.0	24.0	41.0
40	34.33356765533882	37.0	33.0	40.0	24.0	41.0
41	34.28262123524188	37.0	33.0	40.0	23.0	41.0
42	34.20986842032144	36.0	33.0	40.0	23.0	41.0
43	34.159696096399145	36.0	33.0	40.0	23.0	41.0
44	33.99424845986536	36.0	33.0	40.0	23.0	41.0
45	33.8650566429455	36.0	33.0	40.0	23.0	41.0
46	33.75974344241069	36.0	33.0	40.0	23.0	41.0
47	33.70025695769645	36.0	32.0	40.0	23.0	41.0
48	33.6046380197364	35.0	32.0	40.0	23.0	41.0
49	33.46405704194123	36.0	32.0	40.0	22.0	41.0
50	33.32209180458323	35.0	32.0	39.0	21.0	41.0
51	32.955480300836115	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	6.0
14	18.0
15	62.0
16	176.0
17	446.0
18	950.0
19	1782.0
20	3243.0
21	5367.0
22	8322.0
23	12280.0
24	18001.0
25	25387.0
26	32137.0
27	37790.0
28	43688.0
29	51654.0
30	62170.0
31	74621.0
32	90355.0
33	113037.0
34	164775.0
35	195167.0
36	157266.0
37	180947.0
38	234882.0
39	284953.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.719503778233946	26.85380196252552	29.70801070064317	12.718683558597357
2	29.81053815521712	28.239395215830015	28.611439285408647	13.338627343544216
3	27.959598070149895	26.9750010836235	31.412411545758363	13.652989300468235
4	24.00398328885846	29.260946542351896	31.968227047464932	14.76684312132471
5	21.72292802850541	33.688910030352574	30.744177051854997	13.84398488928702
6	20.780731284710683	42.95189045066512	26.359614074435488	9.907764190188706
7	89.87100990376312	3.427139934137919	5.406503296993973	1.2953468651049893
8	90.14174906836165	2.829813316677021	5.014176018244886	2.0142615967164543
9	85.80197586242538	5.36332506815714	6.615271422680963	2.219427646736515
10	41.87349056802988	32.81906599433849	13.484555308699331	11.822888128932304
11	32.46174809032196	26.67514301051726	24.12962804484312	16.733480854317655
12	31.294991214314056	25.229755967986982	25.799186226533994	17.676066591164968
13	21.791557517068462	34.60571108485716	27.6491816141878	15.953549783886576
14	17.498630188750543	37.58739840335023	27.11387160339602	17.800099804503205
15	16.206728690682727	30.35940735241326	37.18123408601637	16.25262987088765
16	18.27411562429495	26.660194563210815	37.59829020882258	17.467399603671648
17	17.98203741224039	28.664397910996165	26.63040880947009	26.723155867293354
18	20.00141148907652	30.174302229819318	30.701165534326414	19.123120746777747
19	23.79442717438781	31.661811662900845	25.277490972582655	19.26627019012869
20	25.323725575404083	29.959744776101154	26.20662866389778	18.509900984596985
21	21.837903260762047	32.041746734403326	27.706308022481572	18.41404198235305
22	22.079801369033262	28.83494357933624	26.387843855965876	22.697411195664614
23	18.728181657532737	33.582826067869284	25.73933686687213	21.949655407725846
24	19.315450026062532	30.02509560893528	33.24240157642213	17.417052788580055
25	18.61009448085543	31.80168245052286	29.460722260071865	20.127500808549843
26	17.700406442169513	37.0697042207969	26.491538289697576	18.738351047336007
27	18.397148569783685	36.319670044978714	26.90609374288004	18.377087642357566
28	16.887744384885288	33.58065882086203	32.031299492419635	17.500297301833044
29	17.122029343413068	29.969691884160092	31.2924905446903	21.615788227736537
30	18.041331067541417	33.96603979510069	29.2112665724933	18.781362564864594
31	22.814553674928508	32.337381454367225	24.949625399690362	19.898439471013905
32	22.59382790280508	31.968671610953596	26.55072080412644	18.886779682114877
33	21.853685264609744	32.363944122815106	24.8317049343213	20.950665678253845
34	17.85072447177522	32.29703731777065	27.203784568979028	22.648453641475104
35	17.963588027460688	31.10655186555511	28.882789724804088	22.047070382180117
36	22.94975654591952	30.420090268616374	25.992849196284784	20.637303989179326
37	18.161585491225985	34.22722084469286	28.48546110680749	19.12573255727367
38	18.322017340198876	34.45267010388337	25.19224592363066	22.033066632287092
39	18.8118707342744	33.57943627126819	27.064636197026093	20.544056797431313
40	21.13377026514878	30.7797421309484	26.38223124192145	21.704256361981376
41	16.701250001389262	30.172468405428564	28.643558997464876	24.4827225957173
42	19.330287332496813	31.167568204374728	25.941168690727185	23.560975772401278
43	19.770127334097243	31.174959072373827	26.472699911865288	22.582213681663646
44	18.359471814119114	33.37671532043581	26.652636983903466	21.611175881541612
45	17.941026430410812	35.8619919333955	24.155857290674504	22.04112434551919
46	20.15839797101224	34.59748666031682	25.145400046012323	20.098715322658624
47	19.369131067319138	31.69370909321274	26.747884711350483	22.189275128117643
48	19.73989701686785	29.931570565006854	29.14174795695292	21.18678446117238
49	19.414254261418893	29.634102020652197	28.685736958452207	22.265906759476703
50	18.149304424851543	32.70836968566027	27.71153164347342	21.430794246014766
51	16.833285357523515	33.431285488669744	26.053754394232232	23.68167475957451
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2103.0
1	2003.0
2	1903.0
3	7833.0
4	13763.0
5	10374.0
6	6985.0
7	7248.0
8	7511.0
9	8964.5
10	10418.0
11	12029.5
12	13641.0
13	14248.0
14	14855.0
15	14254.5
16	13654.0
17	13101.0
18	12548.0
19	12186.0
20	11824.0
21	12319.5
22	12815.0
23	13877.5
24	14940.0
25	15837.0
26	19947.0
27	23160.0
28	26372.0
29	29584.0
30	35005.5
31	40427.0
32	47070.5
33	53714.0
34	59530.5
35	65347.0
36	70935.5
37	76524.0
38	81743.0
39	86962.0
40	102776.5
41	118591.0
42	134950.5
43	151310.0
44	156851.0
45	162392.0
46	165355.0
47	168318.0
48	167152.0
49	165986.0
50	153051.5
51	140117.0
52	122211.0
53	104305.0
54	90397.5
55	76490.0
56	65955.0
57	55420.0
58	50386.0
59	45352.0
60	39312.0
61	33272.0
62	27502.0
63	21732.0
64	16928.5
65	12125.0
66	9507.5
67	6890.0
68	5017.0
69	3144.0
70	2616.0
71	2088.0
72	1562.5
73	1037.0
74	793.5
75	507.0
76	464.0
77	341.0
78	218.0
79	189.5
80	161.0
81	113.5
82	66.0
83	49.0
84	32.0
85	26.0
86	20.0
87	18.0
88	16.0
89	10.5
90	5.0
91	4.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1799518.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.75444449127971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.32835750911725	20.421228040882305
2	8.84192349935134	4.731215029190741
3	3.3684053739462385	2.703594438041188
4	1.9296598539981125	2.0650790980337366
5	1.1793406267548903	1.577630166741237
6	0.8191605127537364	1.314971068075084
7	0.58694191034367	1.0992313331926775
8	0.4703322456916069	1.0066782367852025
9	0.4040246483799745	0.9728509525371748
>10	4.504388248510707	28.210495235712823
>50	1.2486435503647368	23.470567143840427
>100	0.3152135169932002	11.125847746165094
>500	0.002971709007304661	0.5498437100509939
>1k	4.2452985818638013E-4	0.21631426271268697
>5k	2.1226492909319006E-4	0.5344535380386184
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9411	0.522973373981255	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2069	0.11497523225663761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06801821376613071	0.0
2	0.0	0.0	0.0	0.23678562815153836	0.0
3	0.0	0.0	0.0	0.3899377499974993	0.0
4	0.0	0.0	0.0	0.6674009373621158	0.0
5	0.0	0.0	0.0	1.2366089141647931	0.0
6	0.0	0.0	0.0	2.0774451825433253	0.0
7	0.0	0.0	0.0	2.5061155264909827	0.0
8	0.0	0.0	0.0	3.3490634714406857	0.0
9	0.0	0.0	0.0	3.693655745594098	0.0
10	0.0	0.0	0.0	4.202736510554493	0.0
11	0.0	0.0	0.0	4.917094466407116	0.0
12	0.0	0.0	0.0	5.43778945250895	0.0
13	0.0	0.0	0.0	5.729756523691344	0.0
14	0.0	0.0	0.0	5.884575758619808	0.0
15	0.0	0.0	0.0	6.056677399170222	0.0
16	0.0	0.0	0.0	6.3568688948929655	0.0
17	0.0	0.0	0.0	6.715298207631155	0.0
18	0.0	0.0	0.0	7.087786840698453	0.0
19	0.0	0.0	0.0	7.337242528277017	0.0
20	0.0	0.0	0.0	7.591588414230922	0.0
21	0.0	0.0	0.0	7.919565127995385	0.0
22	0.0	0.0	0.0	8.290497788852349	0.0
23	0.0	0.0	0.0	8.692994457404705	0.0
24	0.0	0.0	0.0	9.011357485726734	0.0
25	0.0	0.0	0.0	9.280151685062334	0.0
26	0.0	0.0	0.0	9.539109917211164	0.0
27	0.0	0.0	0.0	9.787732048248476	0.0
28	0.0	0.0	0.0	10.055803831914991	0.0
29	0.0	0.0	0.0	10.337712654166282	0.0
30	0.0	0.0	0.0	10.659576619961568	0.0
31	0.0	0.0	0.0	10.956211607774971	0.0
32	0.0	0.0	0.0	11.250012503348119	0.0
33	0.0	0.0	0.0	11.533977431734497	0.0
34	0.0	0.0	0.0	11.819720614075546	0.0
35	0.0	0.0	0.0	12.141195586818249	0.0
36	5.557043608344012E-5	0.0	0.0	12.433662791925393	0.0
37	5.557043608344012E-5	0.0	0.0	12.739633613000814	0.0
38	1.1114087216688024E-4	0.0	0.0	13.04115879918956	0.0
39	1.1114087216688024E-4	0.0	0.0	13.343739823663892	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.0343504E-4	45.000004	1
CGCATAA	20	7.0343504E-4	45.000004	32
TACGCAC	30	2.166189E-6	45.000004	30
GCGATAC	80	0.0	45.000004	9
CCGACGG	20	7.0343504E-4	45.000004	2
GGACCGT	20	7.0343504E-4	45.000004	8
ACGCCGA	25	3.891688E-5	45.0	12
TAGCGCG	25	3.891688E-5	45.0	1
CCAGTCG	25	3.891688E-5	45.0	28
CCTTGCG	25	3.891688E-5	45.0	14
TGGTACG	50	2.1827873E-11	45.0	1
CGTTTTT	4660	0.0	42.392704	1
CGGTCTA	170	0.0	42.35294	31
ATCCGCG	55	6.184564E-11	40.909092	1
TCTACGG	160	0.0	40.781254	2
TACGGGA	630	0.0	40.35714	4
TGTAGCG	95	0.0	40.263157	1
TACGTAG	95	0.0	40.263157	1
GTACCGG	45	1.929402E-8	40.000004	2
CGGACGA	45	1.929402E-8	40.000004	31
>>END_MODULE