FastQCFastQC Report
Thu 26 May 2016
SRR1548029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548029_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2226830
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98660.4430513330608983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77250.041.0679631
TAAGGGA38600.038.6463744
GGCGATA7650.038.5294158
AGGGCGA17350.038.5158466
CGTAAGG3450.038.478262
TTAGGGA34500.038.2173924
GCGAGAC4600.038.15217221
TACGGGA12400.038.104844
GGGCGAT35750.037.7622387
ACGGGAT13850.037.6895335
AAGGGAT44900.037.4331865
CACAACG2850.037.10526312
TATGGGA35050.036.9115524
TAGGGAT43800.036.883565
AGGGATC29050.036.7125666
TAGGGAG32450.036.5408335
CACGACC4500.036.527
ATGGGAC23300.036.405585
GACCGAT10850.036.290329
ATTGGGA26600.036.2875944
Produced by FastQC (version 0.11.5)