##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548029_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2226830
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.868372080491103	31.0	31.0	31.0	30.0	33.0
2	31.2076835681215	31.0	31.0	33.0	30.0	34.0
3	31.344656754220125	31.0	31.0	34.0	30.0	34.0
4	35.25680092328557	37.0	35.0	37.0	33.0	37.0
5	35.110232932015464	37.0	35.0	37.0	32.0	37.0
6	35.14859239367172	37.0	35.0	37.0	32.0	37.0
7	35.5174018672283	35.0	35.0	37.0	35.0	37.0
8	35.60157039378848	37.0	35.0	37.0	35.0	37.0
9	37.42815796446069	39.0	37.0	39.0	35.0	39.0
10	36.539476744969306	38.0	35.0	39.0	32.0	39.0
11	36.20326832313199	38.0	35.0	39.0	31.0	39.0
12	35.804721959017975	37.0	35.0	39.0	30.0	39.0
13	35.6542079997126	37.0	35.0	39.0	30.0	39.0
14	36.69090994822236	38.0	35.0	40.0	31.0	41.0
15	36.848569940228934	38.0	35.0	40.0	31.0	41.0
16	36.92345172285267	38.0	35.0	40.0	31.0	41.0
17	36.85440334466484	38.0	35.0	40.0	31.0	41.0
18	36.73661617635832	38.0	35.0	40.0	31.0	41.0
19	36.6915323576564	38.0	35.0	40.0	31.0	41.0
20	36.567887086126916	38.0	35.0	40.0	30.0	41.0
21	36.46027626716004	38.0	35.0	40.0	30.0	41.0
22	36.333879999820375	38.0	35.0	40.0	30.0	41.0
23	36.2407619800344	38.0	34.0	40.0	30.0	41.0
24	36.12680132744754	38.0	34.0	40.0	30.0	41.0
25	35.96294553243849	38.0	34.0	40.0	29.0	41.0
26	35.740929033648726	38.0	34.0	40.0	29.0	41.0
27	35.82683410947401	38.0	34.0	40.0	29.0	41.0
28	35.85254150518899	38.0	34.0	40.0	29.0	41.0
29	35.82536116362722	38.0	34.0	40.0	29.0	41.0
30	35.74489476071366	38.0	34.0	40.0	29.0	41.0
31	35.67231939573295	38.0	34.0	40.0	29.0	41.0
32	35.528025938217105	38.0	34.0	40.0	27.0	41.0
33	35.36686949609984	38.0	34.0	40.0	27.0	41.0
34	35.18273464970384	38.0	34.0	40.0	26.0	41.0
35	35.01786710256284	38.0	34.0	40.0	25.0	41.0
36	34.94940161574974	38.0	33.0	40.0	25.0	41.0
37	34.908665681708975	38.0	33.0	40.0	25.0	41.0
38	34.84339846328638	38.0	33.0	40.0	24.0	41.0
39	34.79636927830144	38.0	33.0	40.0	24.0	41.0
40	34.65030918390717	38.0	33.0	40.0	24.0	41.0
41	34.567449243992584	37.0	33.0	40.0	24.0	41.0
42	34.53345832416485	37.0	33.0	40.0	24.0	41.0
43	34.488525841667304	37.0	33.0	40.0	24.0	41.0
44	34.338376526272775	37.0	33.0	40.0	23.0	41.0
45	34.217502458651985	37.0	33.0	40.0	23.0	41.0
46	34.153322884997955	37.0	33.0	40.0	23.0	41.0
47	34.10848246161584	37.0	33.0	40.0	23.0	41.0
48	34.008058540616034	37.0	33.0	40.0	23.0	41.0
49	33.85403914982284	36.0	32.0	40.0	23.0	41.0
50	33.76106977182811	36.0	32.0	40.0	23.0	41.0
51	33.42827786584517	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	13.0
14	33.0
15	78.0
16	201.0
17	602.0
18	1302.0
19	2431.0
20	4058.0
21	6603.0
22	10052.0
23	14559.0
24	21325.0
25	29877.0
26	38873.0
27	45287.0
28	50788.0
29	58879.0
30	69883.0
31	85095.0
32	102604.0
33	127266.0
34	179118.0
35	205745.0
36	195367.0
37	242484.0
38	330532.0
39	403722.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.667828258106816	24.974066273581727	28.958205161597427	13.399900306714027
2	30.922701777863598	26.624349411495263	29.10761036989802	13.345338440743118
3	28.641297270110428	26.12161682750816	31.67731708302834	13.55976881935307
4	25.135461620330247	29.101368312803398	31.033576878342757	14.729593188523596
5	23.083082228998173	33.205184050870514	29.494483189107385	14.217250531023923
6	21.580722372161322	42.24094340385211	26.268911412186828	9.909422811799733
7	90.98579595209333	2.681300323778645	5.322364078084093	1.010539646043928
8	91.46293161130397	2.1794209706174246	5.172195452728767	1.1854519653498472
9	86.28530242542088	5.500419879380106	6.325763529321951	1.888514165877054
10	36.55200441883754	40.52635360579838	12.723827144416052	10.19781483094803
11	31.281732328017853	25.218404637983145	26.565656112051663	16.934206921947343
12	29.699213680433623	23.193777701935037	28.519195448238076	18.587813169393264
13	24.46262175379351	27.64360099334031	29.48186435426144	18.411912898604744
14	20.006915660378205	30.185779785614532	30.068797348697473	19.73850720530979
15	19.68830130723944	27.36962408446087	34.77027882685252	18.17179578144717
16	23.055778842569932	26.433135892726433	32.97588949313599	17.535195771567654
17	23.054521449773894	25.792449356259795	28.9602259714482	22.19280322251811
18	23.32966593767822	26.543247576150854	30.537355792763705	19.58973069340722
19	25.307904060929665	28.02140262166398	27.246444497334778	19.424248820071583
20	27.005653776893613	27.428407197675618	27.78514749666566	17.780791528765107
21	25.87341647094749	27.08329778204892	28.924884252502437	18.118401494501153
22	25.37620743388584	24.571565858193036	28.935751718811048	21.116474989110078
23	22.264384798121096	27.780791528765103	29.214937826416925	20.739885846696872
24	22.07258748983982	26.79284004616428	32.01353493531163	19.121037528684273
25	21.510577816896664	28.51129183637727	29.74192012861332	20.236210218112742
26	21.439086055064823	30.82413116403138	27.540404970294098	20.1963778106097
27	20.538658092445313	30.086984637354448	29.322265282935835	20.052091987264404
28	19.539704422879158	29.116232491927985	31.233008357171403	20.111054728021447
29	20.319198142651214	27.081905668596168	31.472047709075234	21.12684847967739
30	21.499081654190036	28.230623801547488	29.911398714764935	20.358895829497538
31	23.528693254536716	28.056025830440586	28.053241603535067	20.36203931148763
32	24.445377509733568	27.523789422632174	28.14642339109856	19.884409676535704
33	23.66803932046901	27.665111391529663	28.10780346950598	20.55904581849535
34	21.15136763920012	28.53545174081542	29.14793675314236	21.165243866842104
35	21.62630286101768	28.166406955178434	29.394161206737827	20.813128977066054
36	23.72816964025094	28.599668587184475	27.346946107246623	20.325215665317963
37	21.501776067324403	30.294858610670772	28.18603126417373	20.01733405783109
38	21.854384932841754	29.759748162185705	27.292069893076704	21.093797011895834
39	21.461045522109906	29.54810201048127	27.678942712286076	21.311909755122755
40	23.24640857182632	27.95000067360328	27.504389648064738	21.29920110650566
41	20.174014181594462	27.701710503271464	28.779610477674545	23.34466483745953
42	21.52360081371277	28.343160456792837	27.30998774042024	22.823250989074154
43	21.701791335665497	27.484450990870428	28.261923900791707	22.551833772672364
44	20.78950795525478	28.677492219882073	28.336783679041506	22.19621614582164
45	20.475653731986725	30.141007620698478	26.87551362250374	22.507825024811055
46	21.531234984260138	29.80977443271377	27.328175029077208	21.330815553948888
47	20.960468468630296	28.593651064517722	28.697296156419664	21.748584310432317
48	21.638742068321335	27.377303161893813	29.42254235841982	21.561412411365037
49	21.227844065330537	27.55482008056295	29.10720620792786	22.11012964617865
50	20.599866177480994	29.51159271251061	28.55058536125344	21.337955748754954
51	20.168176286470004	29.07720840836526	28.053870299933088	22.700745005231653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1422.0
1	1788.5
2	2155.0
3	10302.5
4	18450.0
5	14482.5
6	10515.0
7	9949.5
8	9384.0
9	9774.5
10	10165.0
11	10096.0
12	10027.0
13	9957.0
14	9887.0
15	9762.5
16	9638.0
17	9463.0
18	9288.0
19	9680.5
20	10073.0
21	10622.5
22	11172.0
23	12963.0
24	14754.0
25	17619.0
26	25290.0
27	30096.0
28	34840.0
29	39584.0
30	45085.5
31	50587.0
32	58514.5
33	66442.0
34	74812.0
35	83182.0
36	88547.0
37	93912.0
38	100069.0
39	106226.0
40	120086.0
41	133946.0
42	145499.5
43	157053.0
44	158521.0
45	159989.0
46	162775.5
47	165562.0
48	162108.0
49	158654.0
50	155684.5
51	152715.0
52	144059.5
53	135404.0
54	124359.0
55	113314.0
56	105122.0
57	96930.0
58	91675.5
59	86421.0
60	80285.5
61	74150.0
62	65726.0
63	57302.0
64	49171.5
65	41041.0
66	34475.5
67	27910.0
68	23121.0
69	18332.0
70	15155.5
71	11979.0
72	10039.0
73	8099.0
74	6483.5
75	3813.0
76	2758.0
77	2057.0
78	1356.0
79	1073.5
80	791.0
81	603.0
82	415.0
83	309.0
84	203.0
85	150.0
86	97.0
87	77.5
88	58.0
89	40.5
90	23.0
91	17.5
92	12.0
93	7.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2226830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.91807206183039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.04603476658328	17.199104327351844
2	6.36212802265861	2.91615416979761
3	2.4060056934985496	1.6542303559432185
4	1.4684315694736918	1.3461448210825908
5	1.054179978412865	1.207988635570242
6	0.8157864081708254	1.1217751013712454
7	0.6958470116241183	1.1163230369487611
8	0.618092646769474	1.1332393455640224
9	0.5537666738274569	1.1422138082596003
>10	10.076875530857558	57.142552135222616
>50	0.8613684725087757	11.909796399021548
>100	0.04007362509356242	1.4148057367017863
>500	8.054860121453463E-4	0.13545161823201493
>1k	4.0274300607267313E-4	0.10490000962227256
>5k	2.0137150303633656E-4	0.45532049931075275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9866	0.4430513330608983	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018007661114678712	0.0
2	0.0	0.0	0.0	0.05730118599084798	0.0
3	0.0	0.0	0.0	0.09443918035952453	0.0
4	0.0	0.0	0.0	0.15371626931557417	0.0
5	0.0	0.0	0.0	0.2798597108894707	0.0
6	0.0	0.0	0.0	0.49415536884270467	0.0
7	0.0	0.0	0.0	0.6097905991925742	0.0
8	4.490688557276487E-5	0.0	0.0	0.8507609471760305	0.0
9	4.490688557276487E-5	0.0	0.0	0.9640610194761162	0.0
10	4.490688557276487E-5	0.0	0.0	1.136368739418815	0.0
11	4.490688557276487E-5	0.0	0.0	1.3361145664464733	0.0
12	4.490688557276487E-5	0.0	0.0	1.4853850540903437	0.0
13	4.490688557276487E-5	0.0	0.0	1.5792404449374222	0.0
14	4.490688557276487E-5	0.0	0.0	1.6368110722417069	0.0
15	4.490688557276487E-5	0.0	0.0	1.700174687784878	0.0
16	4.490688557276487E-5	0.0	0.0	1.8079512131595137	0.0
17	4.490688557276487E-5	0.0	0.0	1.9327923550518	0.0
18	4.490688557276487E-5	0.0	0.0	2.072991651809972	0.0
19	4.490688557276487E-5	0.0	0.0	2.1703048728461534	0.0
20	4.490688557276487E-5	0.0	0.0	2.2758809608277235	0.0
21	4.490688557276487E-5	0.0	0.0	2.4078622975260795	0.0
22	4.490688557276487E-5	0.0	0.0	2.5588841537072877	0.0
23	4.490688557276487E-5	0.0	0.0	2.724141492615063	0.0
24	4.490688557276487E-5	0.0	0.0	2.856437177512428	0.0
25	4.490688557276487E-5	0.0	0.0	2.9793024164395128	0.0
26	4.490688557276487E-5	0.0	0.0	3.1011347969984238	0.0
27	4.490688557276487E-5	0.0	0.0	3.2243143841245177	0.0
28	4.490688557276487E-5	0.0	0.0	3.358765599529376	0.0
29	4.490688557276487E-5	0.0	0.0	3.5083953422578285	0.0
30	4.490688557276487E-5	0.0	0.0	3.6852386576433767	0.0
31	4.490688557276487E-5	0.0	0.0	3.848250652272513	0.0
32	4.490688557276487E-5	0.0	0.0	4.009690905906603	0.0
33	4.490688557276487E-5	0.0	0.0	4.166595564097843	0.0
34	4.490688557276487E-5	0.0	0.0	4.335310733194721	0.0
35	4.490688557276487E-5	0.0	0.0	4.519384057157484	0.0
36	4.490688557276487E-5	0.0	0.0	4.700807874871454	0.0
37	4.490688557276487E-5	0.0	0.0	4.888922818535766	0.0
38	4.490688557276487E-5	0.0	0.0	5.081663171414073	0.0
39	4.490688557276487E-5	0.0	0.0	5.328830669606571	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7725	0.0	41.067963	1
TAAGGGA	3860	0.0	38.646374	4
GGCGATA	765	0.0	38.529415	8
AGGGCGA	1735	0.0	38.515846	6
CGTAAGG	345	0.0	38.47826	2
TTAGGGA	3450	0.0	38.217392	4
GCGAGAC	460	0.0	38.152172	21
TACGGGA	1240	0.0	38.10484	4
GGGCGAT	3575	0.0	37.762238	7
ACGGGAT	1385	0.0	37.689533	5
AAGGGAT	4490	0.0	37.433186	5
CACAACG	285	0.0	37.105263	12
TATGGGA	3505	0.0	36.911552	4
TAGGGAT	4380	0.0	36.88356	5
AGGGATC	2905	0.0	36.712566	6
TAGGGAG	3245	0.0	36.540833	5
CACGACC	450	0.0	36.5	27
ATGGGAC	2330	0.0	36.40558	5
GACCGAT	1085	0.0	36.29032	9
ATTGGGA	2660	0.0	36.287594	4
>>END_MODULE