FastQCFastQC Report
Thu 26 May 2016
SRR1548028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548028_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2312156
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163370.7065699719223097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC302.1664582E-645.00000422
CGTTTTT85700.042.29581
CGCGTAA501.0822987E-940.531
CACGACC7700.040.32467327
ACACGAC7650.040.29411726
GACACGA7700.040.03246725
TACGCCC451.9297659E-840.00000442
ACTCGCG403.4602454E-739.3751
TACGGGA13650.039.2307664
TTAAGCG1100.038.8636321
GCGAGAC8050.038.8509321
ATTCGCG700.038.5714261
CTACGCA356.2510335E-638.57142623
CCGACGG356.2510335E-638.5714261
GGGCGAT40600.038.4605947
AGGGCGA19850.038.4256946
CGTAAGG3850.037.987012
AGGGATT57650.037.7797056
TGCGAAG2150.037.674421
TAGGGAT54750.037.6438375
Produced by FastQC (version 0.11.5)