##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548028_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2312156
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95667939360493	31.0	31.0	33.0	30.0	33.0
2	31.29811829305635	31.0	31.0	33.0	30.0	34.0
3	31.400907637719946	31.0	31.0	34.0	30.0	34.0
4	35.31890971024446	37.0	35.0	37.0	33.0	37.0
5	35.19071853283256	37.0	35.0	37.0	32.0	37.0
6	35.2423642695389	37.0	35.0	37.0	32.0	37.0
7	35.62886673736547	36.0	35.0	37.0	35.0	37.0
8	35.73850726335074	37.0	35.0	37.0	35.0	37.0
9	37.57763230508668	39.0	37.0	39.0	35.0	39.0
10	36.74017410589943	39.0	37.0	39.0	32.0	39.0
11	36.34682607920919	38.0	35.0	39.0	32.0	39.0
12	35.99001364959804	37.0	35.0	39.0	31.0	39.0
13	35.8030894109221	38.0	35.0	39.0	30.0	39.0
14	36.83954629358919	39.0	35.0	40.0	31.0	41.0
15	37.0254273500577	39.0	35.0	41.0	31.0	41.0
16	37.122463190199966	39.0	35.0	41.0	32.0	41.0
17	37.04783025020803	39.0	35.0	41.0	32.0	41.0
18	36.94489472163643	38.0	35.0	40.0	31.0	41.0
19	36.87276118047398	38.0	35.0	40.0	31.0	41.0
20	36.74375647663912	38.0	35.0	40.0	31.0	41.0
21	36.62477229045099	38.0	35.0	40.0	31.0	41.0
22	36.51806928252246	38.0	35.0	40.0	31.0	41.0
23	36.447461157465156	38.0	35.0	40.0	30.0	41.0
24	36.355583273792945	38.0	35.0	40.0	30.0	41.0
25	36.199183359600305	38.0	35.0	40.0	30.0	41.0
26	35.99440435679946	38.0	34.0	40.0	30.0	41.0
27	36.06786047308226	38.0	34.0	40.0	30.0	41.0
28	36.107129017246244	38.0	35.0	40.0	30.0	41.0
29	36.10706111525347	38.0	35.0	40.0	30.0	41.0
30	36.03943721790398	38.0	35.0	40.0	30.0	41.0
31	35.92941090480054	38.0	34.0	40.0	30.0	41.0
32	35.78499244860641	38.0	34.0	40.0	29.0	41.0
33	35.62170113089255	38.0	34.0	40.0	29.0	41.0
34	35.45805430083437	38.0	34.0	40.0	27.0	41.0
35	35.2733569880233	38.0	34.0	40.0	27.0	41.0
36	35.16066389984067	38.0	34.0	40.0	25.0	41.0
37	35.11940024808015	38.0	34.0	40.0	26.0	41.0
38	35.03636692333908	38.0	34.0	40.0	25.0	41.0
39	35.019541501524984	38.0	34.0	40.0	25.0	41.0
40	34.84747958182752	38.0	34.0	40.0	24.0	41.0
41	34.77175545248677	38.0	34.0	40.0	24.0	41.0
42	34.7253195718628	38.0	33.0	40.0	24.0	41.0
43	34.696545994301424	37.0	33.0	40.0	24.0	41.0
44	34.564819155800905	37.0	33.0	40.0	24.0	41.0
45	34.45049944726913	37.0	33.0	40.0	23.0	41.0
46	34.394060348869196	37.0	33.0	40.0	23.0	41.0
47	34.338327085196674	37.0	33.0	40.0	23.0	41.0
48	34.24494411276748	37.0	33.0	40.0	23.0	41.0
49	34.11120659678672	37.0	33.0	40.0	23.0	41.0
50	33.989524928248784	36.0	33.0	40.0	23.0	41.0
51	33.68476434981031	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	22.0
15	51.0
16	172.0
17	446.0
18	968.0
19	1957.0
20	3437.0
21	5630.0
22	8649.0
23	13011.0
24	19628.0
25	28593.0
26	37341.0
27	43011.0
28	47811.0
29	55678.0
30	66707.0
31	81846.0
32	101872.0
33	129568.0
34	187931.0
35	227536.0
36	205757.0
37	255577.0
38	352238.0
39	436659.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.027015478194375	24.802867972576244	30.032705405690617	14.137411143538758
2	30.3661604147817	26.69525758642583	29.793664441326623	13.144917557465845
3	27.59022315103306	26.833829551293253	32.68745707469565	12.888490222978035
4	24.355104067372615	29.32124822027579	32.174818654104655	14.148829058246934
5	22.73030020465747	33.147590387499804	30.479993564448073	13.64211584339465
6	21.10912931480402	41.688320338247074	27.015045697608635	10.18750464934027
7	90.89075304607475	2.3857386785320713	5.73101468932027	0.992493586072912
8	91.82818979342224	1.7766102287215912	5.367501154766375	1.0276988230897914
9	87.01649023681793	5.1543234972034755	6.382354823809466	1.446831442169127
10	35.6835351939921	44.36482659474534	11.197125107475447	8.754513103787115
11	28.27188130904662	25.917758144346664	29.76473040746386	16.04563013914286
12	28.632453865569623	24.438446194806925	28.683531734017947	18.245568205605505
13	22.442473604722174	30.241168848468703	29.973453348303487	17.342904198505636
14	17.82055363046438	33.488311342314276	30.191431720005053	18.499703307216294
15	17.561012319237975	28.348043990111393	37.11682948728373	16.9741142033669
16	21.500279392912937	26.907267502711758	35.237285027480844	16.355168076894465
17	21.09918188911129	26.01450767162769	29.28003127816635	23.60627916109467
18	21.84519556638912	27.202057300631964	31.967176955188144	18.985570177790773
19	24.123848044855105	28.96551962756838	28.098925851023893	18.81170647655262
20	26.303415513486115	29.157288695053445	27.802579064734385	16.73671672672605
21	23.683523084082562	28.096374120085322	30.19900041346691	18.021102382365203
22	23.425106264456204	25.17083622385341	30.08694050055446	21.317117011135927
23	19.795160880148224	30.22417172543721	29.673170841413814	20.307496553000746
24	19.562780366030665	28.700312608664813	33.65815282359841	18.07875420170611
25	20.094967640591722	29.56037568399364	31.244734351834392	19.099922323580245
26	19.827554888164986	33.630170282627994	27.757210153640155	18.785064675566872
27	19.19918033212292	32.08191834806994	30.035213886952267	18.683687432854875
28	16.98933808964447	31.111611846259507	33.473822700544424	18.425227363551596
29	18.16832428261761	28.531292871242254	33.142789673361136	20.157593172779
30	20.119014460961974	29.37375332806264	31.69725572149976	18.809976489475623
31	23.451099320288076	29.03268637583277	28.59876236724512	18.917451936634034
32	23.30193118457405	29.021311710801523	29.27510081499691	18.401656289627518
33	22.588441264343757	29.30706232624442	28.527616648703635	19.576879760708188
34	19.722717671298994	29.964241167118484	29.635976119258387	20.677065042324134
35	19.706845039867552	29.63848460052003	30.405777118844924	20.24889324076749
36	23.214437088154952	29.397367651663643	28.708789545342096	18.67940571483931
37	20.26982608439915	31.221725523710337	29.415273017910554	19.093175373979957
38	19.72310691839132	32.0370684330988	27.750895700809114	20.48892894770076
39	20.388157200465713	30.634481410423863	28.433894598807345	20.54346679030308
40	22.830206958354022	29.0335946190482	27.907286532569604	20.228911890028183
41	18.993268620283406	28.878760775657007	29.26653737896578	22.86143322509381
42	20.856853949300998	29.275619811120013	27.909622015123546	21.957904224455444
43	20.838083589515584	29.483131761005744	28.089973167900435	21.588811481578233
44	19.778596253885983	30.99613520886999	28.719082968450227	20.5061855687938
45	19.22638437890869	31.949747335387407	27.055138148118036	21.768730137585873
46	20.90572608422615	31.101794169597554	27.5592131326779	20.433266613498397
47	20.104915066284455	29.98807173910411	28.830148138793405	21.076865055818033
48	20.80504083634495	29.278950036243227	29.568809370993996	20.34719975641782
49	20.78397824368252	28.67687128377151	29.288508214843635	21.250642257702335
50	19.476843257980864	31.238463148680278	28.88568072396499	20.399012869373866
51	19.24969595477122	31.317220810360546	27.826971882520034	21.606111352348197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1807.0
1	1951.5
2	2096.0
3	13659.0
4	25222.0
5	18737.5
6	12253.0
7	11476.5
8	10700.0
9	12072.0
10	13444.0
11	14585.5
12	15727.0
13	16340.5
14	16954.0
15	16672.5
16	16391.0
17	16057.5
18	15724.0
19	15899.5
20	16075.0
21	16339.0
22	16603.0
23	17563.5
24	18524.0
25	21419.0
26	29044.0
27	33774.0
28	38974.0
29	44174.0
30	51570.5
31	58967.0
32	64027.5
33	69088.0
34	77005.0
35	84922.0
36	93459.0
37	101996.0
38	109401.0
39	116806.0
40	132590.0
41	148374.0
42	164755.0
43	181136.0
44	185993.5
45	190851.0
46	188326.5
47	185802.0
48	180882.0
49	175962.0
50	169872.5
51	163783.0
52	149993.5
53	136204.0
54	123675.5
55	111147.0
56	99887.5
57	88628.0
58	78510.5
59	68393.0
60	61166.5
61	53940.0
62	44828.0
63	35716.0
64	29411.5
65	23107.0
66	18073.5
67	13040.0
68	9975.5
69	6911.0
70	5962.5
71	5014.0
72	4178.5
73	3343.0
74	2803.5
75	2075.0
76	1886.0
77	1231.5
78	577.0
79	405.0
80	233.0
81	205.0
82	177.0
83	116.0
84	55.0
85	41.5
86	28.0
87	21.0
88	14.0
89	10.5
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2312156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.53544730969376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57818963078144	16.80652862876825
2	7.771775811301672	3.5028088859668265
3	3.189530007296573	2.1563245626635728
4	1.840785147367836	1.6593166678789895
5	1.2487405306093673	1.4070463215513211
6	0.8590759520340613	1.1615796511253154
7	0.666997188798983	1.052175600272534
8	0.5642116787374678	1.0171810046161656
9	0.4687095760924679	0.9506321960022638
>10	6.951307836161321	38.62153867798091
>50	1.7064338988948906	25.664782290949105
>100	0.151086916062827	4.739608791750858
>500	0.002366869395902817	0.3310540452108954
>1k	5.917173489757042E-4	0.20326398345185617
>5k	0.0	0.0
>10k+	1.9723911632523475E-4	0.7261586918112779
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16337	0.7065699719223097	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031875011893661154	0.0
2	0.0	0.0	0.0	0.10003650272732463	0.0
3	0.0	0.0	0.0	0.1568665782066608	0.0
4	0.0	0.0	0.0	0.23778672373317372	0.0
5	0.0	0.0	0.0	0.44157920140336554	0.0
6	0.0	0.0	0.0	0.7771534446637683	0.0
7	0.0	0.0	0.0	0.9687927631180595	0.0
8	0.0	0.0	0.0	1.3039777592861381	0.0
9	0.0	0.0	0.0	1.468802278046983	0.0
10	0.0	0.0	0.0	1.6991933070259966	0.0
11	0.0	0.0	0.0	1.9916043727153359	0.0
12	0.0	0.0	0.0	2.20659851670908	0.0
13	0.0	0.0	0.0	2.34145100936096	0.0
14	0.0	0.0	0.0	2.423495646487521	0.0
15	0.0	0.0	0.0	2.5074432693987774	0.0
16	0.0	0.0	0.0	2.6446312446046027	0.0
17	0.0	0.0	0.0	2.818797693581229	0.0
18	0.0	0.0	0.0	3.010480261712445	0.0
19	0.0	0.0	0.0	3.149225225287567	0.0
20	0.0	0.0	0.0	3.2823477308624502	0.0
21	0.0	0.0	0.0	3.449810479915715	0.0
22	0.0	0.0	0.0	3.6541652033859306	0.0
23	0.0	0.0	0.0	3.8680348557796274	0.0
24	0.0	0.0	0.0	4.031734882940424	0.0
25	4.324967692491337E-5	0.0	0.0	4.177097047085058	0.0
26	4.324967692491337E-5	0.0	0.0	4.3221564634912175	0.0
27	4.324967692491337E-5	0.0	0.0	4.472838337897616	0.0
28	4.324967692491337E-5	0.0	0.0	4.636365366350713	0.0
29	4.324967692491337E-5	0.0	0.0	4.820003494573895	0.0
30	4.324967692491337E-5	0.0	0.0	5.039409105613981	0.0
31	8.649935384982675E-5	0.0	0.0	5.232389164052945	0.0
32	8.649935384982675E-5	0.0	0.0	5.424547478630335	0.0
33	8.649935384982675E-5	0.0	0.0	5.607104364930394	0.0
34	8.649935384982675E-5	0.0	0.0	5.80116566529248	0.0
35	8.649935384982675E-5	0.0	0.0	6.0153813151015765	0.0
36	8.649935384982675E-5	0.0	0.0	6.212513342525331	0.0
37	8.649935384982675E-5	0.0	0.0	6.415527326010874	0.0
38	8.649935384982675E-5	0.0	0.0	6.633375948681663	0.0
39	8.649935384982675E-5	0.0	0.0	6.920856551201562	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGC	30	2.1664582E-6	45.000004	22
CGTTTTT	8570	0.0	42.2958	1
CGCGTAA	50	1.0822987E-9	40.5	31
CACGACC	770	0.0	40.324673	27
ACACGAC	765	0.0	40.294117	26
GACACGA	770	0.0	40.032467	25
TACGCCC	45	1.9297659E-8	40.000004	42
ACTCGCG	40	3.4602454E-7	39.375	1
TACGGGA	1365	0.0	39.230766	4
TTAAGCG	110	0.0	38.863632	1
GCGAGAC	805	0.0	38.85093	21
ATTCGCG	70	0.0	38.571426	1
CTACGCA	35	6.2510335E-6	38.571426	23
CCGACGG	35	6.2510335E-6	38.571426	1
GGGCGAT	4060	0.0	38.460594	7
AGGGCGA	1985	0.0	38.425694	6
CGTAAGG	385	0.0	37.98701	2
AGGGATT	5765	0.0	37.779705	6
TGCGAAG	215	0.0	37.67442	1
TAGGGAT	5475	0.0	37.643837	5
>>END_MODULE