FastQCFastQC Report
Thu 26 May 2016
SRR1548027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548027_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277345
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33340.26101014213074775No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29950.2344707185607647No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG253.8910504E-545.030
CTTCGAA253.8910504E-545.028
ACCGGCA207.0335815E-445.043
TCCGCGA207.0335815E-445.031
CGTATCG207.0335815E-445.041
GGTCGTA207.0335815E-445.025
TCCGATC351.2120654E-745.014
AACCCGC207.0335815E-445.018
CGGCACT207.0335815E-445.023
TCTAGCG253.8910504E-545.01
GCCGATC253.8910504E-545.09
GCCGAAT207.0335815E-445.09
GTATCGC207.0335815E-445.09
ACGTCGT207.0335815E-445.015
TATAGCG207.0335815E-445.01
TTCGGCC253.8910504E-545.015
CCCGGTA253.8910504E-545.045
TCGACGA207.0335815E-445.032
ATCGCTA207.0335815E-445.014
TACACCG207.0335815E-445.041