##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548027_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1277345
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.910411047915794	31.0	31.0	33.0	30.0	33.0
2	31.259811562263916	31.0	31.0	33.0	30.0	34.0
3	31.46297515549832	31.0	31.0	34.0	30.0	34.0
4	35.3250899326337	37.0	35.0	37.0	33.0	37.0
5	35.13606425828574	37.0	35.0	37.0	32.0	37.0
6	35.16715139605979	37.0	35.0	37.0	32.0	37.0
7	35.50737036587609	35.0	35.0	37.0	35.0	37.0
8	35.59356321119196	37.0	35.0	37.0	35.0	37.0
9	37.44084722608223	39.0	37.0	39.0	35.0	39.0
10	36.63834437837859	39.0	37.0	39.0	32.0	39.0
11	36.24989333343772	38.0	35.0	39.0	32.0	39.0
12	35.18393856006013	37.0	35.0	39.0	30.0	39.0
13	34.5634844149388	37.0	33.0	39.0	26.0	39.0
14	35.53896402303215	38.0	33.0	40.0	27.0	41.0
15	35.97240604535188	38.0	34.0	40.0	30.0	41.0
16	36.233186805444106	38.0	34.0	40.0	31.0	41.0
17	36.20713432940983	37.0	34.0	40.0	31.0	41.0
18	36.13414465160157	37.0	35.0	40.0	30.0	41.0
19	36.04336494838904	37.0	34.0	40.0	30.0	41.0
20	35.89637255400851	37.0	34.0	40.0	30.0	41.0
21	35.73007057607772	37.0	34.0	40.0	30.0	41.0
22	35.56629884643538	36.0	34.0	40.0	29.0	41.0
23	35.5784725348281	36.0	34.0	40.0	29.0	41.0
24	35.43708708297289	36.0	34.0	40.0	29.0	41.0
25	35.29003753880119	36.0	34.0	40.0	29.0	41.0
26	35.04223056417804	35.0	34.0	40.0	28.0	41.0
27	35.07928554932301	36.0	34.0	40.0	28.0	41.0
28	35.18623864343619	36.0	34.0	40.0	29.0	41.0
29	35.24249282691834	36.0	34.0	40.0	29.0	41.0
30	35.22482492983493	36.0	34.0	40.0	29.0	41.0
31	35.07310789175986	36.0	34.0	40.0	29.0	41.0
32	34.860213959423646	35.0	34.0	40.0	27.0	41.0
33	34.724482422524844	35.0	33.0	40.0	27.0	41.0
34	34.63162810360553	35.0	33.0	40.0	26.0	41.0
35	34.49105449193444	35.0	33.0	40.0	25.0	41.0
36	34.334323929713584	35.0	33.0	40.0	24.0	41.0
37	34.22865161722166	35.0	33.0	40.0	24.0	41.0
38	34.23687179266369	35.0	33.0	40.0	24.0	41.0
39	34.26191044706011	35.0	33.0	40.0	24.0	41.0
40	34.014004830331665	35.0	33.0	40.0	23.0	41.0
41	34.02462999424588	35.0	33.0	40.0	23.0	41.0
42	33.96240796339282	35.0	33.0	40.0	23.0	41.0
43	33.9041143935272	35.0	33.0	40.0	23.0	41.0
44	33.704128485256525	35.0	33.0	40.0	23.0	41.0
45	33.56139335888112	35.0	32.0	39.0	23.0	41.0
46	33.53745385937237	35.0	32.0	39.0	23.0	41.0
47	33.50878032168286	35.0	32.0	39.0	23.0	41.0
48	33.489762750079265	35.0	32.0	39.0	23.0	41.0
49	33.41099154887677	35.0	32.0	39.0	23.0	41.0
50	33.26870187772293	35.0	32.0	39.0	23.0	41.0
51	32.89790776963154	35.0	31.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	2.0
14	17.0
15	60.0
16	138.0
17	347.0
18	837.0
19	1633.0
20	2671.0
21	4374.0
22	6423.0
23	9386.0
24	13412.0
25	18066.0
26	22978.0
27	26820.0
28	31630.0
29	37545.0
30	44471.0
31	53769.0
32	66177.0
33	85981.0
34	141653.0
35	169267.0
36	93803.0
37	113247.0
38	151343.0
39	181265.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.216468534342717	24.974223878435346	30.715898993615664	13.09340859360627
2	28.306448140478885	28.386066411188832	30.042001182139515	13.265484266192768
3	27.8005550575608	27.08727869134807	30.90331899369395	14.208847257397178
4	24.784142107261548	30.018045242279882	30.66501219326024	14.53280045719833
5	23.289635924515302	33.697317482747415	28.890706895944323	14.122339696792958
6	21.15223373481714	43.54305219028532	25.049536342961375	10.255177731936165
7	91.08948639560964	3.8546359832308417	4.039472499598777	1.0164051215607373
8	91.98877358896775	3.005061279450736	3.465391104204424	1.5407740273770987
9	87.5353173966313	5.977789868829486	4.880122441470394	1.6067702930688261
10	48.77131863357198	33.17623664710787	9.230395860163073	8.822048859157078
11	47.12783155686209	20.56179027592389	18.95525484501055	13.355123322203477
12	42.278867494686246	21.599176416708094	20.69174733529313	15.430208753312534
13	22.463155999358044	41.330102673905635	21.225510727328952	14.981230599407363
14	16.196642254050396	42.78851837209211	24.603454822307206	16.411384551550288
15	14.785120699576074	24.797998974435252	44.91065452168365	15.506225804305023
16	17.77264560475048	21.253067886906045	42.71391049403254	18.260376014310932
17	17.504354735799645	21.287357761607083	25.66855469743883	35.539732805154436
18	22.521088664378066	24.580203468914036	32.40808082389644	20.490627042811457
19	28.59947782314097	26.20826793074698	23.83843049450227	21.353823751609784
20	31.352453722369443	25.260364271203162	23.36487010165617	20.022311904771225
21	23.249631070697426	29.284570730695307	26.396314229906565	21.069483968700702
22	23.49310483855184	24.85788882408433	24.53965060340002	27.10935573396381
23	20.19861509615648	31.06185094864739	24.233390352645525	24.506143602550605
24	20.76471117826429	24.61496306792605	37.107281118257006	17.51304463555265
25	18.536495621777984	26.510222375317554	33.97993494318293	20.97334705972153
26	18.407556298415855	35.892339187924954	24.616920252555104	21.083184261104087
27	18.364341661806325	35.89241747531012	26.469591222418376	19.273649640465184
28	15.285846815073453	31.005405743945445	36.45577349893725	17.252973942043848
29	15.85296063318837	25.459762241211266	36.33043539529258	22.356841730307785
30	18.61877566358345	31.866332118574075	30.3199213994653	19.19497081837718
31	29.794613045026992	28.144001816267334	23.003495531747493	19.057889606958184
32	30.079579127017368	27.144037045590657	24.92999150581871	17.846392321573262
33	28.075422066865258	28.528627739569185	22.985097996234376	20.410852197331184
34	20.070850083571784	28.817038466506702	26.937358348762473	24.174753101159045
35	19.645201570444947	27.884557421839833	29.69283944431614	22.77740156339908
36	30.16859188394678	26.00511216625109	25.233120261166718	18.593175688635412
37	20.402475447118828	33.12057431625755	27.723598557946367	18.75335167867726
38	20.02677428572547	33.56007969655809	22.92098062778654	23.492165389929895
39	20.38008525496244	32.29080632092348	26.242870954988668	21.086237469125415
40	24.62279180644227	26.92123114741906	24.187905381866294	24.268071664272377
41	17.821810082632336	24.58881508128188	27.04907444738892	30.540300388696867
42	21.576786224551707	25.570460603830604	23.921649984929676	28.931103186688013
43	22.220699967510736	26.9956824507083	24.646904321072224	26.13671326070874
44	19.70634401825662	30.616239152304193	27.33333594291284	22.34408088652635
45	17.507016506895162	36.82372420919955	22.302901721931036	23.36635756197425
46	22.556631137241702	32.09821935342448	24.81561363609675	20.529535873237066
47	21.549698789285586	27.515354113414936	26.537466385353998	24.39748071194548
48	22.836743401352024	24.51679068693266	30.42106870109485	22.225397210620468
49	20.800175363742763	24.01841319299015	31.08118793278245	24.10022351048464
50	19.741886491120255	32.53404522662241	26.691379384582863	21.032688897674472
51	18.55418857082464	33.11360673897811	24.004086601505467	24.328118088691777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1605.0
1	1565.0
2	1525.0
3	3644.0
4	5763.0
5	4672.0
6	3581.0
7	3740.0
8	3899.0
9	4366.5
10	4834.0
11	5067.5
12	5301.0
13	5416.5
14	5532.0
15	5421.5
16	5311.0
17	5282.5
18	5254.0
19	5256.5
20	5259.0
21	5339.0
22	5419.0
23	5706.0
24	5993.0
25	6282.5
26	7374.5
27	8177.0
28	10571.5
29	12966.0
30	17151.5
31	21337.0
32	21746.5
33	22156.0
34	26727.0
35	31298.0
36	34051.5
37	36805.0
38	44283.5
39	51762.0
40	70265.5
41	88769.0
42	107703.5
43	126638.0
44	128227.5
45	129817.0
46	123076.0
47	116335.0
48	109760.0
49	103185.0
50	97285.0
51	91385.0
52	86147.5
53	80910.0
54	72297.5
55	63685.0
56	58618.5
57	53552.0
58	49182.5
59	44813.0
60	42496.0
61	40179.0
62	34578.5
63	28978.0
64	24934.5
65	20891.0
66	17578.0
67	14265.0
68	11475.0
69	8685.0
70	7716.0
71	6747.0
72	5072.0
73	3397.0
74	2832.5
75	1808.5
76	1349.0
77	896.5
78	444.0
79	424.5
80	405.0
81	274.0
82	143.0
83	112.5
84	82.0
85	71.0
86	60.0
87	35.0
88	10.0
89	6.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1277345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.363321041529435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70189942013884	15.003182087547573
2	6.596941681050356	2.422835159627491
3	1.9280685618294209	1.062172259728608
4	0.9232046921276185	0.678124165943432
5	0.5660167771119492	0.5196973896499266
6	0.389827080872982	0.42951119020516965
7	0.2896932318773929	0.3723810874365956
8	0.22859101807319346	0.3358152201670487
9	0.20829551240591943	0.34424976292377985
>10	3.5168936573433904	17.8397657388886
>50	2.606370350645518	34.62883158300761
>100	1.0385537029205154	25.283179434958637
>500	0.00434177969448376	0.4762242701790155
>1k	0.001302533908345128	0.6040306497365178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3334	0.26101014213074775	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2995	0.2344707185607647	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017458086891168793	0.0
2	0.0	0.0	0.0	0.09629348374949602	0.0
3	0.0	0.0	0.0	0.1525038262959498	0.0
4	0.0	0.0	0.0	0.31996054315787825	0.0
5	0.0	0.0	0.0	0.5264043778305783	0.0
6	0.0	0.0	0.0	0.8154414038493907	0.0
7	0.0	0.0	0.0	0.922851696291918	0.0
8	0.0	0.0	0.0	1.2128281709326767	0.0
9	0.0	0.0	0.0	1.2890017966954894	0.0
10	0.0	0.0	0.0	1.4456548544050354	0.0
11	0.0	0.0	0.0	1.7007151552634565	0.0
12	0.0	0.0	0.0	1.8847687977797698	0.0
13	0.0	0.0	0.0	1.9613338604683934	0.0
14	0.0	0.0	0.0	1.999538104427543	0.0
15	0.0	0.0	0.0	2.0605239774688906	0.0
16	0.0	0.0	0.0	2.196509165495618	0.0
17	0.0	0.0	0.0	2.3460380711554043	0.0
18	0.0	0.0	0.0	2.534710669396287	0.0
19	0.0	0.0	0.0	2.6311607279161073	0.0
20	0.0	0.0	0.0	2.7295679710649825	0.0
21	0.0	0.0	0.0	2.86555315909171	0.0
22	0.0	0.0	0.0	3.008819073938521	0.0
23	0.0	0.0	0.0	3.161870911930606	0.0
24	0.0	0.0	0.0	3.291123384833385	0.0
25	0.0	0.0	0.0	3.3874168685828807	0.0
26	0.0	0.0	0.0	3.4851195252652962	0.0
27	0.0	0.0	0.0	3.5739757074243843	0.0
28	0.0	0.0	0.0	3.677941354919775	0.0
29	0.0	0.0	0.0	3.796077019129523	0.0
30	0.0	0.0	0.0	3.95492212362361	0.0
31	0.0	0.0	0.0	4.0819042623566855	0.0
32	0.0	0.0	0.0	4.206772641690381	0.0
33	0.0	0.0	0.0	4.325456317596264	0.0
34	0.0	0.0	0.0	4.461206643467505	0.0
35	0.0	0.0	0.0	4.637274972697274	0.0
36	0.0	0.0	0.0	4.77427789673111	0.0
37	0.0	0.0	0.0	4.913786017090136	0.0
38	0.0	0.0	0.0	5.055173034693055	0.0
39	0.0	0.0	0.0	5.225134947880173	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	25	3.8910504E-5	45.0	30
CTTCGAA	25	3.8910504E-5	45.0	28
ACCGGCA	20	7.0335815E-4	45.0	43
TCCGCGA	20	7.0335815E-4	45.0	31
CGTATCG	20	7.0335815E-4	45.0	41
GGTCGTA	20	7.0335815E-4	45.0	25
TCCGATC	35	1.2120654E-7	45.0	14
AACCCGC	20	7.0335815E-4	45.0	18
CGGCACT	20	7.0335815E-4	45.0	23
TCTAGCG	25	3.8910504E-5	45.0	1
GCCGATC	25	3.8910504E-5	45.0	9
GCCGAAT	20	7.0335815E-4	45.0	9
GTATCGC	20	7.0335815E-4	45.0	9
ACGTCGT	20	7.0335815E-4	45.0	15
TATAGCG	20	7.0335815E-4	45.0	1
TTCGGCC	25	3.8910504E-5	45.0	15
CCCGGTA	25	3.8910504E-5	45.0	45
TCGACGA	20	7.0335815E-4	45.0	32
ATCGCTA	20	7.0335815E-4	45.0	14
TACACCG	20	7.0335815E-4	45.0	41
>>END_MODULE