FastQCFastQC Report
Thu 26 May 2016
SRR1548026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548026_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1290437
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.3369401218346963No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25220.19543766956465133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAG950.045.0000041
GGACGTA351.2120836E-745.0000048
TTACGTC302.1657088E-645.00000434
TAATCGT1300.045.00000421
AGCCGAC253.8910737E-545.012
TCGATGC253.8910737E-545.035
ATATCGT207.033609E-445.022
ATATCCG207.033609E-445.01
CCCGTAC253.8910737E-545.010
TCGTGCG502.1827873E-1145.01
CCGAGTA253.8910737E-545.023
AGTTATC253.8910737E-545.026
TTACGCC207.033609E-445.012
GAACCGT253.8910737E-545.016
TTGCACG207.033609E-445.01
TACGTAG453.8562575E-1045.01
CGACGGT2200.043.9772728
TACGGGA4750.043.1052674
GACGGTC2250.043.029
CGCATCG850.042.3529421
Produced by FastQC (version 0.11.5)